公开(公告)号：WO9813478A3

公开(公告)日：1998-08-20

申请号：PCT/EP9704923

申请日：1997-09-04

申请人： MOGEN INT ,  STUIVER MAARTEN HENDRIK ,  CUSTERS JEROME HUBERTUS HENRIC ,  SELA BUURLAGE MARIANNE BEATRIX ,  MELCHERS LEO SJOERD ,  DEVENTER TROOST JOHANNA PIETER ,  LAGEWEG WESSEL ,  PONSTEIN ANNE SILENE

发明人： STUIVER MAARTEN HENDRIK ,  CUSTERS JEROME HUBERTUS HENRIC ,  SELA-BUURLAGE MARIANNE BEATRIX ,  MELCHERS LEO SJOERD ,  VAN DEVENTER-TROOST JOHANNA PI ,  LAGEWEG WESSEL ,  PONSTEIN ANNE SILENE

IPC分类号： A01H5/00 ,  C07K14/415 ,  C07K16/40 ,  C12N5/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N15/09 ,  C12N15/62 ,  C12N15/82 ,  C12Q1/68

CPC分类号： C12N9/0004 ,  A01N65/12 ,  C07K14/415 ,  C12N9/0006 ,  C12N15/8282

摘要： The present invention provides an isolated protein obtainable from a plant source which has antifungal activity, specifically anti-Phytophthora activity and/or anti-Pythium activity and a molecular weight of about 55-65 kDa as judged by SDS PAGE-electrophoresis, an isolated DNA sequence comprising an open reading frame capable of encoding a protein according to the invention, preferably characterised in that it comprises an open reading frame which is capable of encoding a protein depicted in SEQ ID NO. 16, SEQ ID NO. 57, SEQ ID NO. 70, SEQ ID NO. 72 or SEQ ID NO. 74 or muteins thereof, and DNA capable of hybridising therewith under stringent conditions. The invention further comprises plants incorporating chimeric DNA capable of encoding a protein according to the invention, and wherein the protein is expressed. Also shown is the carbohydrate and preferably hexose oxidating activity of said protein. Also methods are provided for combating fungi, especially Phytophthora and Pythium species, using a protein or a host cell capable of producing the protein.

公开(公告)号：WO9118984A3

公开(公告)日：1992-05-14

申请号：PCT/NL9100089

申请日：1991-05-31

申请人： MOGEN INT

发明人： WOLOSHUK CHARLES PETER ,  MELCHERS LEO SJOERD ,  CORNELISSEN BERNARDUS JOHANNES ,  MEULENHOFF ELISABETH JOSINE SO ,  SELA-BUURLAGE MARIANNE BEATRIX ,  VAN DEN ELZEN PETRUS JOSEPHUS

IPC分类号： A01H5/00 ,  A01N63/00 ,  A01N63/02 ,  A01N65/00 ,  A01N65/08 ,  A01N65/10 ,  A01N65/20 ,  A01N65/38 ,  A61K38/00 ,  A61P31/04 ,  C07K1/14 ,  C07K1/20 ,  C07K14/415 ,  C12N5/10 ,  C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  C12P21/00 ,  C12R1/91 ,  A61K37/02

CPC分类号： A01N65/20 ,  A01N65/00 ,  A01N65/08 ,  A01N65/10 ,  A01N65/38 ,  A61K38/00 ,  C07K14/415 ,  C12N15/8282 ,  A01N2300/00

摘要： New methods are provided for the identification and purification of proteins from plants with an inhibitory effect on pathogens and/or pests, such as insects, nematodes, fungi, and bacteria, using a combination of non-denaturing protein fractionation techniques on leaf-extracts of plants which were treated to develop induced resistance and an antipathogenic assay. Also provided are antifungal proteins obtained by the said method, recombinant nucleotide sequences encoding the said proteins, as well as plants with reduced susceptibility to fungi due to production and desired targeting of the said proteins in the plant.

公开(公告)号：WO9505467A2

公开(公告)日：1995-02-23

申请号：PCT/EP9402761

申请日：1994-08-17

申请人： MOGEN INT ,  UNIV LEIDEN ,  GROOT MARION DE ,  BOL JOHN FERDINAND ,  CORNELISSEN BERNARDUS JOHANNES ,  LINTHORST HUBERTUS J M ,  MELCHERS LEO SJOERD ,  PONSTEIN ANNE SILENE ,  SELA BUURLAGE MARIANNE BEATRIX

发明人： APOTHEKER-DE GROOT MARION ,  BOL JOHN FERDINAND ,  CORNELISSEN BERNARDUS JOHANNES ,  LINTHORST HUBERTUS JOSEPHUS MA ,  MELCHERS LEO SJOERD ,  PONSTEIN ANNE SILENE ,  SELA-BUURLAGE MARIANNE BEATRIX

IPC分类号： A01H5/00 ,  A01N65/00 ,  A01N65/36 ,  A01N65/38 ,  A01P3/00 ,  A61P31/10 ,  C07K14/415 ,  C12N1/21 ,  C12N5/10 ,  C12N9/24 ,  C12N15/09 ,  C12N15/56 ,  C12N15/82 ,  C12R1/01

CPC分类号： A01N65/385 ,  A01N65/00 ,  A01N65/38 ,  C12N9/2442 ,  C12N15/8282 ,  C12Y302/01014 ,  A01N2300/00

摘要： The present invention provides a novel class of plant chitinases, which have endo-chitinase activity, antifungal activity and a molecular weight of about 40 to 43 kDa as judged by Sodium Dodecyl Sulphate Polyacrylamide electrophoreses. The invention further provides antifungal compositions comprising as active ingredient a chitinase according to the invention. According to a different aspect of the invention an isolated DNA sequence is provided which comprises an open reading frame encoding a novel chitinase. The invention also encompasses a plant expressible recombinant DNA sequence which comprises a DNA sequence encoding a chitinase according to the invention, as well as plant cells and plants which have such recombinant DNA incorporated into their genome.

摘要翻译： 本发明提供了一类新颖的植物几丁质酶，其具有内切几丁质酶活性，抗真菌活性和约40至43kDa的分子量，由十二烷基硫酸钠聚丙烯酰胺电泳鉴定。 本发明进一步提供了包含根据本发明的几丁质酶作为活性成分的抗真菌组合物。 根据本发明的不同方面，提供了分离的DNA序列，其包含编码新几丁质酶的开放阅读框。 本发明还包括植物可表达的重组DNA序列，其包含编码根据本发明的几丁质酶的DNA序列，以及将这种重组DNA并入其基因组中的植物细胞和植物。