公开(公告)号：WO2020154301A1

公开(公告)日：2020-07-30

申请号：PCT/US2020/014437

申请日：2020-01-21

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： BOWEN, David J. ,  CHAY, Catherine A. ,  CHEN, Danqi ,  CICHE, Todd A. ,  HOWE, Arlene R. ,  LUTKE, Jennifer L. ,  WIGGINS, Barbara E. ,  ZHANG, Yuanji

IPC: C07K14/325 ,  C12N15/82 ,  A01N63/02

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC7941, TIC7941PL_1, TIC7941PL_2, and TIC7941PL_3. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC7941, TIC7941PL_1, TIC7941PL_2, and TIC7941PL_3 pesticidal proteins are also provided. Also disclosed are methods and compositions to improve the insecticidal activity of a pesticidal protein against an insect pest species. Further disclosed are method and compositions to reduce expression of a pesticidal protein in the reproductive tissues of a transgenic plant.

公开(公告)号：WO2018118776A1

公开(公告)日：2018-06-28

申请号：PCT/US2017/067019

申请日：2017-12-18

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： BOWEN, David J. ,  CHAY, Catherine A. ,  CICHE, Todd A. ,  FLASINSKI, Stanislaw ,  HOWE, Arlene R. ,  MILLIGAN, Jason S. ,  ROBERTS, James K. ,  YIN, Yong

IPC: A01N37/18 ,  A01N37/46 ,  A01N37/00

Abstract: Pesticidal proteins exhibiting toxic activity against Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran pest species are disclosed, and include, but are not limited to, TIC6280, TIC6281, TIC6282, TIC6283, TIC8808, TIC9480, TIC9257, TIC7106, TIC7017, TIC7107, TIC7108, TIC7109, TIC7110, TIC7111, TIC7589, TIC9258, and TIC9259. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the pesticidal proteins provided. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran, Coleopteran, Hemipteran and Thysanopteran infestation are provided which contain recombinant nucleic acid sequences encoding the disclosed pesticidal proteins. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran species pests using the disclosed pesticidal proteins are also provided.

公开(公告)号：WO2017035364A1

公开(公告)日：2017-03-02

申请号：PCT/US2016/048714

申请日：2016-08-25

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： BOWEN, David J. ,  CHAY, Catherine A. ,  CICHE, Todd A. ,  KESANAPALLI, Uma R. ,  LUTKE, Jennifer L.

IPC: A01N63/02 ,  A61K35/742 ,  C07K14/325 ,  C12N15/32 ,  C12N15/82

CPC classification number: C12N15/8286 ,  A01N37/46 ,  A01N63/02 ,  C07K14/32 ,  C12Q1/6895 ,  C12Q2600/13 ,  G01N33/5308 ,  G01N33/68 ,  G01N2333/415 ,  Y02A40/162

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC6757, TIC6757PL, TIC7472, TIC7472PL, TIC7473, and TIC7473PL. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC6757, TIC6757PL, TIC7472, TIC7472PL, TIC7473, and TIC7473PL pesticidal proteins are also provided.

Abstract translation: 公开了对鳞翅目害虫物质具有毒性活性的杀虫蛋白，包括但不限于TIC6757，TIC6757PL，TIC7472，TIC7472PL，TIC7473和TIC7473PL。 提供了包含编码一种或多种所公开的杀虫蛋白的重组核酸序列的DNA构建体。 提供了耐鳞翅目感染的转基因植物，植物细胞，种子和植物部分，其含有编码本发明的杀虫蛋白的重组核酸序列。 使用TIC6757，TIC6757PL，TIC7472，TIC7472PL，TIC7473和TIC7473PL杀虫蛋白中的任何一种检测生物样品中本发明的重组核酸序列或蛋白质存在的方法以及控制鳞翅目昆虫害虫的方法也是 提供。