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    PESTICIDAL GENES AND METHODS OF USE
    4.
    发明申请
    PESTICIDAL GENES AND METHODS OF USE 审中-公开

    公开(公告)号：WO2019126479A1

    公开(公告)日：2019-06-27

    申请号：PCT/US2018/066766

    申请日：2018-12-20

    申请人： AGBIOME, INC.

    发明人： ROBERTS, Kira Bulazel ,  THAYER, Rebecca E. ,  PARKS, Jessica

    IPC分类号： C12N15/82 ,  C12N15/31 ,  C12N15/32 ,  A01N63/02 ,  C07K14/32 ,  C07K14/325

    CPC分类号： C07K14/325 ,  A01N63/02 ,  C07K14/32 ,  C12N15/8285 ,  C12N15/8286

    摘要： Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptide sequences having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Methods are provided for producing the polypeptides disclosed herein, and for using those polypeptides for controlling a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

    NOVEL INSECT INHIBITORY PROTEINS
    5.
    发明申请
    NOVEL INSECT INHIBITORY PROTEINS 审中-公开
    新颖的保守性蛋白质

    公开(公告)号：WO2017030808A1

    公开(公告)日：2017-02-23

    申请号：PCT/US2016/045810

    申请日：2016-08-05

    申请人： MONSANTO TECHNOLOGY LLC

    发明人： BOWEN, David, J. ,  CHAY, Catherine, A. ,  PANOSIAN, Timothy, D. ,  YIN, Yong

    IPC分类号： A01N63/00 ,  A01P7/04 ,  C07K14/325 ,  C12N15/32

    CPC分类号： C12N15/8286 ,  A01N37/46 ,  A01N63/02 ,  C07K14/195 ,  C07K14/325 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/158 ,  G01N33/68 ,  G01N2333/325

    摘要： Pesticidal proteins exhibiting toxic activity against Coleopteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC2421 TIC3008, and TIC7055. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Coleopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using any of the TIC2421, TIC3008, and TIC7055 pesticidal proteins are also provided.

    摘要翻译： 公开了对鞘翅目和鳞翅目害虫物质具有毒性活性的杀虫蛋白，包括但不限于TIC2421 TIC3008和TIC7055。 提供了包含编码一种或多种所公开的杀虫蛋白的重组核酸序列的DNA构建体。 提供了转基因植物，植物细胞，种子和对鳞翅目和鞘翅目昆虫侵染的植物部分，其含有编码本发明的杀虫蛋白的重组核酸序列。 还提供了用于检测生物样品中本发明的重组核酸序列或蛋白质的存在的方法，以及使用任何TIC2421，TIC3008和TIC7055杀虫蛋白质来控制鞘翅目和鳞翅目昆虫害虫的方法。

    ENGINEERING OF SYSTEMS, METHODS AND OPTIMIZED GUIDE COMPOSITIONS WITH NEW ARCHITECTURES FOR SEQUENCE MANIPULATION
    8.
    发明申请
    ENGINEERING OF SYSTEMS, METHODS AND OPTIMIZED GUIDE COMPOSITIONS WITH NEW ARCHITECTURES FOR SEQUENCE MANIPULATION 审中-公开
    系统工程，方法和优化指南组合与新建立的序列操作

    公开(公告)号：WO2015089473A1

    公开(公告)日：2015-06-18

    申请号：PCT/US2014070152

    申请日：2014-12-12

    申请人： BROAD INST INC ,  MASSACHUSETTS INST TECHNOLOGY ,  HARVARD COLLEGE

    发明人： RAN FEI ,  ZHANG FENG

    IPC分类号： C12N9/22 ,  A61K48/00 ,  C12N15/113 ,  C12N15/32 ,  C12N15/62 ,  C12N15/63 ,  C12N15/79

    CPC分类号： C12N9/22 ,  C12N15/113 ,  C12N15/115 ,  C12N15/63 ,  C12N15/907 ,  C12N2310/10 ,  C12N2310/16 ,  C12N2310/20 ,  C12N2310/3519 ,  C12Y301/00

    摘要： The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRJSPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems. In particular the present invention comprehends engineered new guide architectures to be used in optimized CRISPR-Cas enzyme systems.

    摘要翻译： 本发明提供用于改变靶基因序列和相关基因产物的表达的系统，方法和组合物。 提供了关于CRISPR-Cas系统的Cas蛋白的结构信息，使用该信息来生成CRISPR复合物的修饰组分，编码CRISPR复合物的一种或多种组分或修饰组分的载体和载体系统，以及方法 用于设计和使用这些矢量和组件。 还提供了在真核细胞中引导CRJSPR复合物形成的方法和利用CRISPR-Cas系统的方法。 特别地，本发明包括优化的功能性CRISPR-Cas酶系统。 特别地，本发明理解用于优化的CRISPR-Cas酶系统的工程新指南体系结构。

    MOLÉCULAS VARIANTES SINTÉTICAS DE TOXINAS CRY1IA12 COM PROPRIEDADES DE CONTROLAR INSETOS-PRAGA, COMPOSIÇÕES CONTENDO TAIS MUTANTES E MÉTODO DE UTILIZAÇÃO DOS MESMOS
    9.
    发明申请
    MOLÉCULAS VARIANTES SINTÉTICAS DE TOXINAS CRY1IA12 COM PROPRIEDADES DE CONTROLAR INSETOS-PRAGA, COMPOSIÇÕES CONTENDO TAIS MUTANTES E MÉTODO DE UTILIZAÇÃO DOS MESMOS 审中-公开
    的合成分子CRY1IA12毒素含有法SAME和突变此类使用控制昆虫 - 布拉格性能，组成变异

    公开(公告)号：WO2014100880A2

    公开(公告)日：2014-07-03

    申请号：PCT/BR2013/000609

    申请日：2013-12-27

    申请人： EMBRAPA - EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA ,  FUNDAÇÃO UNIVERSIDADE DE BRASÍLIA - FUB - UNB

    发明人： GROSSI DE SÁ, Maria Fátima ,  MATTAR DA SILVA, Maria Cristina ,  GOMES JÚNIOR, José Edilson ,  TRISTAN LOURENÇO, Isabela ,  LIMA PEPINO DE MACEDO, Leonardo ,  LUCENA, Wagner Alexandre ,  CAMPOS DE ASSIS FONSECA, Fernando

    IPC分类号： C12N15/32

    CPC分类号： C07K14/325

    摘要： A presente invenção refere-se ao campo de controle de insetos-praga, utilizando métodos e composições que compreendem análogos mutantes de δ-endotoxinas. Mais especificamente, a presente invenção tem como objetivo disponibilizar moléculas novas a partir do gene Cry1la12 com toxicidade melhorada para tornar as plantas de cana-de-açúcar resistentes à broca gigante da cana-de-açúcar, gerando plantas transgênicas, capazes de expressar toxinas com elevada atividade entomotóxica; disponibilizar δ- endotoxinas melhoradas por processos biotecnológicos; auxiliar para a redução das doses de aplicação de defensivos químicos com redução de custos sociais e ambientais e aumento da produtividade da cultura. São também aspectos da invenção, construções gênicas contendo as moléculas de ácido nucleico, codificantes para Cry1la12 modificado, métodos para a expressão heteróloga das novas moléculas na forma ativa, bem como o uso das mesmas no controle de insetos-praga. A invenção também fornece genes análogos sintéticos os quais são otimizados para transformação e expressão dos mesmos em plantas.

    摘要翻译：

    本发明MAKE-手;所述指虫害控制领域中，使用M＆eacute;并且所有Make-＆otilde; S包含A突变体徽标δ内毒素。 更具体地，本发明MAKE-HANDS，目的是提供摩尔＆eacute;从Cry1la12基因具有改进的毒性以使植物甘蔗至＆ccedil新的细胞;＆uacute;耐＆车差; 巨型甘蔗螟虫，产生能够表达具有高昆虫活动性的毒素的转基因植物; 通过生物技术工艺提供改进的δ-内毒素; 有助于降低化学农药的施用率，同时降低社会和环境成本，提高作物生产力。 S＆手也＆eacute的;本发明MAKE-HANDS的米方面;所述，建筑＆ccedil;＆otilde; S G＆ecirc;含niques摩尔＆eacute;细胞中的核酸，编码修饰的Cry1la12，M＆eacute，所有用于表达＆HAND的异源oacute;原木 活性形式的新分子，以及它们在防治害虫中的用途。 本发明还提供了合成的类似物基因，这些基因被优化用于在植物中转化和表达。