公开(公告)号：WO0119963A3

公开(公告)日：2001-08-09

申请号：PCT/US0040886

申请日：2000-09-13

Applicant: MOTOROLA INC ,  NAIR VIJAY K ,  GORONKIN HERBERT

Inventor： NAIR VIJAY K ,  GORONKIN HERBERT

IPC: G01N33/48 ,  C12M1/00 ,  C12M1/33 ,  C12N1/06 ,  C12N5/06 ,  C12N13/00 ,  H05B6/68 ,  H05B6/80 ,  C12N15/10

CPC classification number: H05B6/806 ,  C12N1/066 ,  C12N13/00

Abstract: Efficient cell lysis in small samples, i.e., samples less than one milliliter, is achieved by exposing the sample to microwave radiation in the frequency range of 18 to 26 GHz. The sample containing cells is supported in a wave-guide cavity, and a microwave source provides microwave radiation to the input port of the wave-guide cavity. A computer controls the frequency and source power level of the microwave radiation produced by the microwave source. The computer also monitors the input power level of the microwave radiation at the input port by means of an input power measuring instrument, the output power level at the output port by means of an output power measuring instrument, and the temperature of the sample by means of a thermocouple. In this way, the computer can control the operating parameters to achieve efficient cell lysis.

Abstract translation: 通过将样品暴露于18-26GHz的频率范围内的微波辐射，实现小样品（即小于1毫升的样品）中的有效细胞裂解。 包含细胞的样品被支撑在波导腔中，并且微波源向波导腔的输入端提供微波辐射。 计算机控制由微波源产生的微波辐射的频率和源功率水平。 计算机还通过输入功率测量仪器监视输入端口处的微波辐射的输入功率电平，借助输出功率测量仪器检测输出端口处的输出功率电平以及通过装置的样本温度 的热电偶。 这样，计算机可以控制操作参数以实现高效的细胞裂解。