公开(公告)号：WO2018236294A1

公开(公告)日：2018-12-27

申请号：PCT/TH2018/000030

申请日：2018-06-05

Applicant: NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY

Inventor： TANAPONGPIPAT, Sutipa ,  PROMDONKOY, Peerada ,  ROONGSAWANG, Niran ,  HARNPICHARNCHAI, Piyanun ,  WONGWISANSRI, Sriwan ,  EURWILAICHITR, Lily

IPC: C12N15/81 ,  C12P21/02

CPC classification number: C12N15/81 ,  C12N2800/102 ,  C12N2820/55 ,  C12N2830/001 ,  C12N2830/36 ,  C12P21/02

Abstract: This invention is a gene expression product for producing target proteins or bio- products from heat-tolerant yeast by methanol induction and non-induction including its process of product usage. The product in this invention consists of Ogataea thermomethanolica yeast cells heat-tolerant species and proper plasmid vectors. For plasmid vector using methanol induction, the important part for gene expression is methanol inducible promoter from O. thermomethanolica , which is AOX promoter. While, the important part for gene expression in non-induction plasmid vector is GAP promoter which is a non-inducible promoter isolated from O. thermomethanolica . Both promoters can work under high temperature which is appropriate to apply with protein production in larger scale. Moreover, using O. thermomethanolica heat- tolerant species as host cell yeast can reduce the cost of production because it does not need an extra cooling system. In addition, there are other advantages, for example, the recombinant proteins can be produced extracellularly as the main proteins without or small amounts of host cell proteins. This makes it easier for purification step. In the case of the target protein is an enzyme, gene expression product from this invention also produce high level of enzyme activity.