公开(公告)号：WO1995007988A1

公开(公告)日：1995-03-23

申请号：PCT/US1994010264

申请日：1994-09-13

Applicant: NOVO NORDISK A/S ,  NOVO NORDISK BIOTECH, INC.

Inventor： NOVO NORDISK A/S ,  NOVO NORDISK BIOTECH, INC. ,  WAHLEITHNER, Jill, Angela ,  CHRISTENSEN, Bj rn, Eggert ,  SCHNEIDER, Palle

IPC: C12N15/53

CPC classification number: C08H6/00 ,  C11D3/38636 ,  C12N9/0061 ,  C12N15/80 ,  C12Y110/03002 ,  D06P1/67391 ,  D06P5/132 ,  D06P5/137

Abstract: The present invention relates to isolated nucleic acid fragments containing a sequence encoding a Rhizoctonia solani laccase having optimum activity at a neutral or basic pH, and the laccase proteins encoded thereby.

Abstract translation: 本发明涉及含有编码在中性或碱性pH下具有最佳活性的丝核菌属漆酶的序列的分离的核酸片段和由其编码的漆酶蛋白质。

公开(公告)号：WO1997009431A1

公开(公告)日：1997-03-13

申请号：PCT/US1996014087

申请日：1996-09-03

Applicant: NOVO NORDISK BIOTECH, INC.

Inventor： NOVO NORDISK BIOTECH, INC. ,  XU, Feng ,  BERKA, Randy, M. ,  WAHLEITHNER, Jill, Angela

IPC: C12N15/53

CPC classification number: C12N9/0057 ,  C12N9/0061

Abstract: The present invention relates to mutants of a blue multi-copper oxidase, comprising (a) a substitution of one or more amino acid residues with other amino acid residues, (b) and insertion of one or more amino acid residues and/or (c) a deletion of one or more amino acid residues, wherein the substitution, insertion or deletion is carried out at a position which is located no greater than 15 ANGSTROM from a Type I (T1) copper site. The present invention also relates to nucleic acid constructs comprising a nucleic acid sequence encoding the mutants of the present invention, host cells comprising the construct of the present invention, and methods for producing mutants of the present invention.

Abstract translation: 本发明涉及蓝色多铜氧化酶的突变体，其包含（a）一个或多个氨基酸残基与其它氨基酸残基的取代，（b）和一个或多个氨基酸残基的插入和/或（c ）一个或多个氨基酸残基的缺失，其中取代，插入或缺失在位于I型（T1）铜位点不超过15 ANGSTROM的位置进行。 本发明还涉及包含编码本发明突变体的核酸序列，包含本发明构建体的宿主细胞和本发明的突变体的制备方法的核酸构建体。