公开(公告)号：WO2022074037A2

公开(公告)日：2022-04-14

申请号：PCT/EP2021/077513

申请日：2021-10-06

Applicant: NOVOZYMES A/S

Inventor： ANDERSEN, Carsten

IPC: C12N9/26 ,  C12N9/2414 ,  C12Y302/01001

Abstract: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：WO2020074517A1

公开(公告)日：2020-04-16

申请号：PCT/EP2019/077229

申请日：2019-10-08

Applicant: NOVOZYMES A/S

Inventor： PEDERSEN, Poul, Erik, ,  FRIIS, Esben, Peter ,  ANDERSEN, Carsten ,  OESTERGAARD, Peter, Rahbek ,  QUIST, Johan

IPC: C07K14/81 ,  C11D3/386

Abstract: Disclosed are protease inhibitors capable of inhibiting the protease activity of a S1 or a S8 protease at ambient pH, but where the inhibitor further has a pH dependent binding to the S1 or the S8 inhibitor meaning that a complex of a S1 and a S8 protease and the inhibitor dissociates when pH is lowered to a pH below 6.0, e.g. to a pH value in the range of 4.0 to 6.0; e.g. in the range of 4.5 to 6.0; e.g. in the range of 4.5 to 5.5; whereby the protease activity is released.

公开(公告)号：WO2019113413A1

公开(公告)日：2019-06-13

申请号：PCT/US2018/064413

申请日：2018-12-07

Applicant: NOVOZYMES A/S ,  STALLINGS, Kendra

Inventor： STALLINGS, Kendra ,  ANDERSEN, Carsten ,  RAO, Vasudeva Prahlada ,  SAIKIA, Rakhi ,  SRIVASTAVA, Vivek

IPC: C12N9/28

CPC classification number: C12Y302/01001 ,  C12N9/2417

Abstract: The present invention relates to alpha-amylase variants comprising a substitution at a position corresponding to position 188 and at least one further substitution at a position corresponding to position 242 or 279 or 275 of SEQ ID NO: 1, in particular one or more combinations of substitutions selected from the group consisting of E188P+S242Y, E188P+S242F, E188P+S242H, E188P+S242W, E188P+S242P, E188P+S242I, E188P+S242T, E188P+S242L, E188P+K279W, E188P+K279Y, E188P+K279F, E188P+K279H, E188P+K279I, E188P+K279L, E188P+K279D, E188P+K279M, E188P+K279S, E188P+K279T E188P+K279N, E188P+K279Q, E188P+K279V, E188P+K279A, E188P+N275F, E188P+N275Y, E188P+N275W, and E188P+N275H, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to a parent alpha amylase selected from the group consisting of: SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, and SEQ ID NO: 27. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：WO2017114891A1

公开(公告)日：2017-07-06

申请号：PCT/EP2016/082828

申请日：2016-12-29

Applicant: NOVOZYMES A/S

Inventor： REGUEIRA, Torsten, Bak ,  PLESNER, Bitten ,  BLICHER, Thomas, Holberg ,  HOUG, Anne, Dorte ,  ARNEHED, Sofia ,  CHRISTENSEN, Lars, Lehmann, Hylling ,  ANDERSEN, Carsten

IPC: C12N9/26

CPC classification number: C12N9/2414

Abstract: The present invention relates to polypeptide having alpha-amylase activity. The present invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.

Abstract translation: 本发明涉及具有α-淀粉酶活性的多肽。 本发明还涉及编码多肽的多核苷酸; 包含所述多核苷酸的核酸构建体，载体和宿主细胞; 和使用这些多肽的方法。

公开(公告)号：WO2016180749A1

公开(公告)日：2016-11-17

申请号：PCT/EP2016/060267

申请日：2016-05-09

Applicant: NOVOZYMES A/S

Inventor： ANDERSEN, Carsten ,  SRIVASTAVA, Vivek ,  BALUMURI, Padmavathi ,  CHAKRABARTI, Partha, Pratim ,  DALAL, Sohel

IPC: C12N9/28

CPC classification number: C12N9/2417 ,  C11D3/386 ,  C11D3/38681 ,  C12Y302/01001

Abstract: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract translation: 本发明涉及α-淀粉酶变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。

公开(公告)号：WO2015189372A1

公开(公告)日：2015-12-17

申请号：PCT/EP2015/063135

申请日：2015-06-12

Applicant: NOVOZYMES A/S

Inventor： ANDERSEN, Carsten ,  LARSEN, Signe Eskildsen ,  FALLAH-ARAGHI, Ali

IPC: C11D3/386 ,  C12N9/28

CPC classification number: C12N9/2417 ,  C11D3/386 ,  C11D3/38681 ,  C12Y302/01001

Abstract: The present invention relates toalpha-amylasevariants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract translation: 本发明涉及α-淀粉酶变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。

公开(公告)号：WO2015150457A1

公开(公告)日：2015-10-08

申请号：PCT/EP2015/057183

申请日：2015-04-01

Applicant: NOVOZYMES A/S

Inventor： ANDERSEN, Carsten ,  DAMAGER, Iben ,  MUNCH, Astrid

IPC: C11D3/386 ,  C12N9/24

CPC classification number: C11D3/386 ,  C12N9/2414 ,  C12N9/2417 ,  C12Y302/01001

Abstract: The present invention relates to polypeptides having alpha-amylase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract translation: 本发明涉及具有α-淀粉酶活性的多肽和编码该多肽的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

公开(公告)号：WO2014183921A1

公开(公告)日：2014-11-20

申请号：PCT/EP2014/056528

申请日：2014-04-01

Applicant: NOVOZYMES A/S

Inventor： ANDERSEN, Carsten ,  DAMAGER, Iben ,  MUNCH, Astrid

IPC: C12N9/28 ,  C11D3/386

CPC classification number: C12N9/2414 ,  C11D3/386 ,  C11D3/38609 ,  C11D3/38618 ,  C12N9/2417 ,  C12Y302/01001

Abstract: The present invention relates to polypeptides having alpha-amylase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract translation: 本发明涉及具有α-淀粉酶活性的多肽和编码该多肽的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

公开(公告)号：WO2007144424A2

公开(公告)日：2007-12-21

申请号：PCT/EP2007/055961

申请日：2007-06-15

Applicant: NOVOZYMES A/S ,  NOVOZYMES NORTH AMERICA, INC.

Inventor： VIKSOE-NIELSEN, Anders ,  ANDERSEN, Carsten ,  LIU, Jim

CPC classification number: C12P7/06 ,  C12P19/14 ,  C12P19/20 ,  Y02E50/17

Abstract: The present invention relates to processes for producing a starch hydrolysate and optionally a fermentation product, such as ethanol.

Abstract translation: 本发明涉及淀粉水解产物和任选的发酵产物如乙醇的生产方法。

公开(公告)号：WO2005035747A1

公开(公告)日：2005-04-21

申请号：PCT/DK2004/000688

申请日：2004-10-08

Applicant: NOVOZYMES A/S ,  DE MARIA, Leonardo ,  ANDERSEN, Carsten ,  CHRISTENSEN, Lars, Lehmann, Hylling ,  LASSEN, Søren, Flensted ,  ØSTERGAARD, Peter Rahbek

Inventor： DE MARIA, Leonardo ,  ANDERSEN, Carsten ,  CHRISTENSEN, Lars, Lehmann, Hylling ,  LASSEN, Søren, Flensted ,  ØSTERGAARD, Peter Rahbek

IPC: C12N9/52

CPC classification number: C12N9/58 ,  A01K2217/05 ,  A23K20/189 ,  C12N9/48 ,  C12N9/52 ,  C12N2310/111

Abstract: The invention relates to a novel 3D structure encoding a Nocardiopsis protease, as well as to variants of parent protease homologous to Nocardiopsis proteases, preferably of improved thermostability and/or with an amended temperature activity profile. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed and detergents. The invention furthermore relates to methods of generating and preparing protease variants of amended properties.

Abstract translation: 本发明涉及编码诺卡氏菌蛋白酶的新型3D结构，以及与诺卡氏菌蛋白酶同源的亲本蛋白酶的变体，优选具有改善的热稳定性和/或具有修改的温度活性分布。 本发明还涉及编码这些变体的DNA序列，其在重组宿主细胞中的产生，以及使用该变体，特别是在动物饲料和洗涤剂领域中的方法。 本发明还涉及生成和制备修饰性质的蛋白酶变体的方法。