摘要:
The present invention relates to isolated polypeptides having phospholipase C activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
摘要:
Provided is a subject matter relates to a process for producing fermentation products from starch-containing material comprising the steps of: i) saccharifying using a carbohydrate-source generating enzyme; ii) fermenting using a fermenting organism; wherein at least one polypeptide having cellobiohydrolase activity, endoglucanase activity, or beta-glucosidase activity are present or added during fermentation or simultaneous saccharification and fermentation. The subject matter also relates to an enzyme blend or composition comprising the polypeptides.
摘要:
The present invention relates to a parent lipase and variants of a parent lipase. The invention also relates to polynucleotides encoding the lipases or lipase variants of the invention, compositions comprising lipase or lipase variants as well as methods of producing said lipases or lipase variants of the invention and the use of lipases, lipase variants or compositions thereof.
摘要:
The present invention relates to a method for modifying one or more characteristics of a lipase, comprising the step of associating a peptide to the lipase, wherein the peptide has at least 50% sequence identity with the amino acid sequence of at least one major lipase contact zone of the propeptide of said lipase.
摘要:
Provided are isolated polypeptides with lipase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors, and host cells comprising the polynucleotides, and methods of using the polypeptides.
摘要:
Isolated polypeptides with lipase activity; polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.
摘要:
The present invention relates to isolated polypeptides with lipase activity, selected from the group consisting of:(a)a polypeptide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 4;(b)a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) SEQ ID NO: 3or the full-length complement of (i);(c)a polypeptide encoded by a polynucleotide having at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to SEQ ID NO: 3;(d)a polypeptide which is a variant of SEQ ID NO: 4 comprising a substitution, deletion, and/or insertion at one or more (e.g. several) positions; and(e)a polypeptide which is a fragment of any of the polypeptides of (a), (b), (c) or (d).The invention also relates to polynucleotides encoding the polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the polypeptides.
摘要翻译:本发明涉及具有脂肪酶活性的分离的多肽,其选自:(a)多肽,其具有至少75%,至少80%,至少85%,至少90%,至少91%,在 与SEQ ID NO:4的至少92%,至少93%,至少94%,至少95%,至少96%,至少97%,至少98%,至少99%或100%的序列同一性 ;(b)由多核苷酸编码的多肽,其在低严格条件,中等严格条件,中等严格条件,高严格条件或非常高严格条件下与(i)SEQ ID NO:3或全长补体 (c)由多核苷酸编码的多肽,所述多核苷酸具有至少75%,至少80%,至少85%,至少90%,至少91%,至少92%,至少93% 与SEQ ID NO:3的至少94%,至少95%,至少96%,至少97%,至少98%,至少99%或100%的序列同一性;(d) SEQ ID NO:4的变体包含取代, 和/或插入在一个或多个(例如, 几个)职位; (e)多肽,其是(a),(b),(c)或(d)的多肽的片段。本发明还涉及编码多肽的多核苷酸; 核酸构建体,载体和包含多核苷酸的宿主细胞; 和使用多肽的方法。
摘要:
The present invention relates to the making of baked products, more particularly making of baked products using polypeptides having lipolytic activity and at least one emulsifier. The invention also relates to compositions comprising polypeptides having lipolytic activity and at least one emulsifier.
摘要:
The present invention is generally directed to methods of making diester-based lubricant compositions, wherein formation of diester species proceeds via esterification of epoxide intermediates, and wherein the epoxide intermediates are generated via an enzymatically-driven mechanism. In some embodiments, the methods for making such diester-based lubricants utilize a biomass precursor and/or low value (e.g., Fischer-Tropsch (FT) olefins and/or alcohols) so as to produce high value diester-based lubricants. In some embodiments, such diester-based lubricants are derived from FT olefins and fatty acids. The fatty acids can be from a bio-based source (i.e., biomass, renewable source) or can be derived from FT alcohols via oxidation.
摘要:
Molecular dynamics (MD) simulation on the three-dimensional structure of Candida anrtarctica lipase B revealed two hitherto unknown lids with a marked mobility, and this discovery was used to design lipolytic enzyme variants with increased lipolytic enzyme activity.