公开(公告)号：WO2018100370A1

公开(公告)日：2018-06-07

申请号：PCT/GB2017/053603

申请日：2017-11-29

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： CLARKE, James ,  WHITE, James ,  MUSCAT, Richard ,  KNOT, Jessica Mary May ,  NATHANI, Ramiz Iqbal ,  HERON, Andrew John ,  BRUCE, Mark John ,  JAYASINGHE, Lakmal Nishantha ,  CAPROTTI, Domenico ,  STODDART, David Jackson ,  BOWEN, Rebecca Victoria ,  WRIGHT, Christopher James ,  MOODY, Paul Richard

IPC: C12Q1/6869

Abstract: Methods of characterizing an analyte using a nanopore. One aspect features methods for characterizing a double-stranded polynucleotide using a nanopore, e.g ., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g ., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

公开(公告)号：WO2019234432A1

公开(公告)日：2019-12-12

申请号：PCT/GB2019/051571

申请日：2019-06-06

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： CLARKE, James Anthony ,  WHITE, James ,  MUSCAT, Richard ,  JOHNSON, Jessica Mary May ,  NATHANI, Ramiz Iqbal ,  HERON, Andrew John ,  BRUCE, Mark John ,  JAYASINGHE, Lakmal Nishantha ,  CAPROTTI, Domenico ,  STODDART, David Jackson ,  BOWEN, Rebecca Victoria ,  WRIGHT, Christopher James ,  MOODY, Paul Richard

IPC: C12Q1/6869

Abstract: Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

公开(公告)号：WO2021111139A1

公开(公告)日：2021-06-10

申请号：PCT/GB2020/053104

申请日：2020-12-03

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： BROWN, Clive Gavin ,  HERON, Andrew John ,  CLARKE, James Anthony ,  MOODY, Paul Richard ,  ACTON, Aaron Luke ,  HYDE, Jason Robert

IPC: G01N33/487 ,  G01N33/543 ,  B81B1/00 ,  C12Q1/68

Abstract: Provided herein is a method of concentrating a tethering complex in a region of an amphiphilic layer, such as a lipid membrane. Also provided herein are methods of assembling a tethering complex; methods of concentrating an analyte in the region of a detector; amphiphilic layers; and arrays and devices for use in the disclosed methods.