公开(公告)号：WO2017149296A1

公开(公告)日：2017-09-08

申请号：PCT/GB2017/050541

申请日：2017-02-28

Applicant: OXFORD UNIVERSITY INNOVATION LIMITED

Inventor： BAYLEY, Hagan ,  OLOF, Sam ,  GRAHAM, Alexander D ,  BURKE, Madeline ,  ARMSTRONG, James ,  PERRIMAN, Adam ,  NICHOLSON, James ,  SZELE, Francis

IPC: C12N5/077 ,  C12N5/00 ,  C12M3/02 ,  C12M1/26

CPC classification number: C12N5/0062 ,  A61L27/38 ,  A61L27/52 ,  B33Y30/00 ,  C12M3/02 ,  C12M25/14 ,  C12M33/00 ,  C12N5/16 ,  C12N2531/00 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/76 ,  C12N2535/10

Abstract: The present invention relates to a process for producing a droplet assembly, which droplet assembly comprises a plurality of droplets, wherein each of said droplets comprises: an aqueous medium comprising a hydrogel compound; and one or more biological cells disposed in the aqueous medium, which process comprises: generating, in a bulk hydrophobic medium, a plurality of droplets, wherein each of said droplets comprises: an aqueous medium comprising a hydrogel compound; and one or more biological cells disposed in the aqueous medium. The invention also relates to a droplet assembly comprising a plurality of droplets, wherein each of said droplets comprises: (i) an aqueous medium comprising a hydrogel compound; (ii) one or more biological cells disposed in the aqueous medium; and (iii) an outer layer of amphipathic molecules around the surface of the aqueous medium, wherein at least one droplet in the droplet assembly contacts at least one other droplet in the droplet assembly forming a layer of amphipathic molecules as an interface between contacting droplets.

Abstract translation: 本发明涉及一种用于产生液滴组件的方法，该液滴组件包括多个液滴，其中每个所述液滴包括：包含水凝胶化合物的水性介质; 以及置于水性介质中的一种或多种生物细胞，该方法包括：在本体疏水性介质中产生多个液滴，其中每个所述液滴包含：包含水凝胶化合物的水性介质; 和置于水性介质中的一种或多种生物细胞。 本发明还涉及包含多个液滴的液滴组件，其中每个所述液滴包含：（i）包含水凝胶化合物的水性介质; （ii）置于含水介质中的一种或多种生物细胞; 和（iii）水性介质表面周围的两亲分子外层，其中液滴组件中的至少一个液滴接触液滴组件中的至少一个其他液滴，形成两亲分子层作为接触液滴之间的界面。