公开(公告)号：WO1996013716A1

公开(公告)日：1996-05-09

申请号：PCT/US1995012147

申请日：1995-09-21

Applicant: PERKIN-ELMER CORPORATION/APPLIED BIOSYSTEMS DIVISION

Inventor： PERKIN-ELMER CORPORATION/APPLIED BIOSYSTEMS DIVISION ,  SMITH, Douglas, H. ,  CONNELL, Charles, R.

IPC: G01N27/447

CPC classification number: G01N27/44721 ,  G01N27/44782

Abstract: A rotary scanning apparatus for scanning a plurality of separation lanes where the separation lanes are disposed in a nonplanar array. The scanning apparatus includes an optical detection system for detecting radiation emanating from the separation lanes, the optical detection system including collection optics for collecting and focusing the radiation, a detector for measuring the intensity of the radiation, and a rotary scanner for providing relative movement between the collection optics and the separation lanes.

Abstract translation: 一种用于扫描多个分离通道的旋转扫描装置，其中分离通道设置在非平面阵列中。 扫描装置包括用于检测从分离通道发出的辐射的光学检测系统，光学检测系统包括用于收集和聚焦辐射的收集光学器件，用于测量辐射强度的检测器和用于提供辐射强度之间的相对运动的旋转扫描仪 收集光学和分离通道。

公开(公告)号：WO1995030139A1

公开(公告)日：1995-11-09

申请号：PCT/US1995004818

申请日：1995-04-19

Applicant: PERKIN-ELMER CORPORATION

Inventor： PERKIN-ELMER CORPORATION ,  WOUDENBERG, Timothy, M. ,  BODNER, Kevin, S. ,  CONNELL, Charles, R. ,  GANZ, Alan, M. ,  MCBRIDE, Lincoln, J. ,  SAVIANO, Paul, G. ,  SHIGEURA, John ,  TRACY, David, H. ,  YOUNG, Eugene, F. ,  LEE, Linda, G.

IPC: G01N21/64

CPC classification number: G01N21/6428 ,  C12Q1/6818 ,  C12Q1/6851 ,  C12Q1/686 ,  G01N21/0303 ,  G01N21/645 ,  G01N2021/6484 ,  C12Q2561/101 ,  C12Q2545/101 ,  C12Q2537/143

Abstract: A system is provided for carrying out real time fluorescence-based measurements of nucleic acid amplification products. In a preferred embodiment of the invention, an excitation beam is focused into a reaction mixture through a surface, the reaction mixture containing: (i) a first fluorescent indicator capable of generating a first fluorescent signal whose intensity is proportional to the amount of an amplification product in the volume of the reaction mixture illuminated by the excitation beam and (ii) a second fluorescent indicator homogeneously distributed throughout the reaction mixture capable of generating a second fluorescent signal proportional to the volume of reaction mixture illuminated by the excitation beam. Preferably, the excitation beam is focused into the reaction mixture by a lens through a portion of a wall of a closed reaction chamber containing the reaction mixture. The same lens is used to collect the first and second fluorescent signals generated by the first and second fluorescent indicators, respectively, in response to the excitation beam. The ratio of the fluorescent intensities of the first and second fluorescent signals provides a stable quantitative indicator of the amount of amplification product synthesized in the course of the amplification reaction.

Abstract translation: 提供了一种用于进行核酸扩增产物的实时基于荧光的测量的系统。 在本发明的优选实施方案中，激发光束通过表面聚焦到反应混合物中，反应混合物包含：（i）第一荧光指示器，其能够产生第一荧光信号，其强度与放大量成比例 由激发束照射的反应混合物的体积的产物和（ii）均匀分布在整个反应混合物中的第二荧光指示剂，其能够产生与由激发束照射的反应混合物的体积成比例的第二荧光信号。 优选地，激发光束通过透镜通过包含反应混合物的封闭反应室的壁的一部分聚焦到反应混合物中。 相同的透镜被用于响应于激发光束分别收集由第一和第二荧光指示器产生的第一和第二荧光信号。 第一和第二荧光信号的荧光强度比提供了在扩增反应过程中合成的扩增产物的量的稳定的定量指标。