公开(公告)号：WO2019175636A1

公开(公告)日：2019-09-19

申请号：PCT/IB2018/051738

申请日：2018-03-15

Applicant: PETIVA PRIVATE LIMITED

Inventor： IYAPPAN, Saravanakumar ,  VENKATA NARAYANAN, Karthikeyan ,  PANDEY, Banibrata

IPC: C12N9/90

Abstract: The present invention provides for a nucleic acid encoding an isomerase and uses of the isomerase for bioconversion of sugar substrates. The invention represents an advancement in the field of enzyme engineering and discloses a modified nucleic acid for achieving optimum expression of a protein having isomerase activity in a heterologous host. The invention also discloses vectors carrying the modified nucleic acid and recombinant host cells carrying the vectors. The invention also discloses the process for producing a recombinant host cell, process for production of the recombinant enzyme and the process for bioconversion of sugars into their respective isomers using the recombinant protein.

公开(公告)号：WO2019175635A1

公开(公告)日：2019-09-19

申请号：PCT/IB2018/051736

申请日：2018-03-15

Applicant: PETIVA PRIVATE LIMITED

Inventor： IYAPPAN, Saravanakumar ,  VENKATA NARAYANAN, Karthikeyan ,  THUMALA, Murali ,  PANDEY, Banibrata

IPC: C12N15/00

Abstract: The present invention provides for a novel whole cell biocatalyst for the production of trehalulose. The invention represents an advancement in the field of enzyme engineering and discloses a novel double deletion mutant whose invertase and permease encoding genes have been made inoperative. Further, the mutant host cell comprises an expression construct encoding sucrose isomerase enzyme fused to a cell surface anchor protein. The recombinant cell can be used as a biocatalyst for effective conversion of sucrose to trehalulose.

公开(公告)号：WO2019175634A1

公开(公告)日：2019-09-19

申请号：PCT/IB2018/051735

申请日：2018-03-15

Applicant: PETIVA PRIVATE LIMITED

Inventor： IYAPPAN, Saravanakumar ,  DASARI, Siva Rama Prasad ,  PANDEY, Banibrata

IPC: C12N15/00 ,  C12P19/12 ,  C12R1/865

Abstract: The present invention provides for a novel whole-cell biocatalyst for the production of isomaltulose. The invention represents an advancement in the field of enzyme engineering and discloses a novel double deletion mutant whose invertase and permease encoding genes have been made inoperative. Further, the mutant host cell comprises an expression construct encoding isomaltulose synthase enzyme fused to a cell surface anchor protein. The recombinant cell can be used as a biocatalyst for effective conversion of sucrose to isomaltulose.

公开(公告)号：WO2017081666A1

公开(公告)日：2017-05-18

申请号：PCT/IB2016/056827

申请日：2016-11-12

Applicant: PETIVA PRIVATE LIMITED

Inventor： BANIBRATA, Pandey ,  KANUMURU, Rahul Raju ,  IYAPPAN, Saravanakumar ,  VENKATA NARAYANAN, Karthikeyan ,  SHEIKH, Humaira Parveen

IPC: C12P19/24

CPC classification number: A23L33/125 ,  A21D2/181 ,  A21D13/062 ,  A23C19/0765 ,  A23G1/40 ,  A23G3/42 ,  A23G9/34 ,  A23L2/60 ,  A23L7/126 ,  A23L27/33 ,  A23L33/20 ,  A23V2002/00 ,  A23V2200/3322 ,  A23V2250/28 ,  A23V2250/60 ,  A23V2250/62 ,  A23V2250/636

Abstract: The present invention provides a process for preparing non-cariogenic, sustained energy release juice. The process comprises contacting juice with an enzyme immobilized on Duolite at 30-50 ºC for 1-5 h; wherein the enzyme is capable of converting cariogenic sugar to non-cariogenic sugar; and separating juice from the enzyme complex.

Abstract translation: 本发明提供了一种制备非龋齿持续释放能量的果汁的方法。 该方法包括在30-50℃下将汁液与固定在Duolite上的酶接触1-5小时; 其中所述酶能够将致龋性糖转化为非致龋性糖; 并从酶复合物中分离果汁。