公开(公告)号：WO2012038839A3

公开(公告)日：2012-03-29

申请号：PCT/IB2011/003160

申请日：2011-09-20

Applicant: POPULATION GENETICS TECHNOLOGIES LTD. ,  CASBON, James ,  BRENNER, Sydney ,  OSBORNE, Robert ,  LICHTENSTEIN, Conrad ,  CLASS, Andreas

Inventor： CASBON, James ,  BRENNER, Sydney ,  OSBORNE, Robert ,  LICHTENSTEIN, Conrad ,  CLASS, Andreas

IPC: C12Q1/68

Abstract: Aspects of the present invention include methods and compositions for determining the number of individual polynucleotide molecules originating from the same genomic region of the same original sample that have been sequenced in a particular sequence analysis configuration or process. In these aspects of the invention, a degenerate base region (DBR) is attached to the starting polynucleotide molecules that are subsequently sequenced (e.g., after certain process steps are performed, e.g., amplification and/or enrichment). The number of different DBR sequences present in a sequencing run can be used to determine/estimate the number of different starting polynucleotides that have been sequenced. DBRs can be used to enhance numerous different nucleic acid sequence analysis applications, including allowing higher confidence allele call determinations in genotyping applications.

公开(公告)号：WO2013128281A1

公开(公告)日：2013-09-06

申请号：PCT/IB2013/000692

申请日：2013-02-26

Applicant: POPULATION GENETICS TECHNOLOGIES LTD ,  MIKAWA, Gi ,  MUSGRAVE-BROWN, Esther

Inventor： MIKAWA, Gi ,  MUSGRAVE-BROWN, Esther ,  OSBORNE, Robert ,  CLAAS, Andreas ,  CASBON, James

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6853 ,  C12Q2521/301 ,  C12Q2525/161 ,  C12Q2525/179

Abstract: Described herein is a method for adding a counter sequence to the individual polynucleotide molecules of an initial nucleic acid sample. After addition of the counter sequence, the sample may be amplified and the number of initial target molecules in the sample can be estimated by counting the number of counter sequences associated with the amplified target molecules.

Abstract translation: 本文描述了将逆序列添加到初始核酸样品的单个多核苷酸分子的方法。 加入反应序列后，样品可以被扩增，并且可以通过计数与扩增的靶分子相关的计数序列的数量来估计样品中初始靶分子的数目。

公开(公告)号：WO2013124743A1

公开(公告)日：2013-08-29

申请号：PCT/IB2013/000824

申请日：2013-02-20

Applicant: POPULATION GENETICS TECHNOLOGIES LTD.

Inventor： OSBORNE, Robert ,  SLATTER, Andrew ,  CASBON, James

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6853 ,  C12Q2525/185 ,  C12Q2525/301 ,  C12Q2535/122 ,  C12Q2525/155 ,  C12Q2525/191

Abstract: Aspects of the present invention are drawn to processes for moving a region of interest in a polynucleotide from a first position to a second position with regard to a domain within the polynucleotide. In certain embodiments, the method results in moving a region of interest into functional proximity to specific domain elements present in the polynucleotide (e.g., primer sites and/or MID). Compositions, kits and systems that find use in carrying out the reflex processes described herein are also provided.

Abstract translation: 本发明的方面涉及将多核苷酸中的感兴趣区域从关于多核苷酸内的结构域的第一位置移至第二位置的方法。 在某些实施方案中，该方法导致将感兴趣的区域移动到存在于多核苷酸（例如，引物位点和/或MID）中的特定结构域元件的功能接近。 还提供了用于实施本文所述的反射过程的组合物，试剂盒和系统。