公开(公告)号：WO2011161549A2

公开(公告)日：2011-12-29

申请号：PCT/IB2011/002202

申请日：2011-06-20

Applicant: POPULATION GENETICS TECHNOLOGIES LTD. ,  OSBORNE, Robert ,  SLATTER, Andrew

Inventor： OSBORNE, Robert ,  SLATTER, Andrew

IPC: C12N15/09

CPC classification number: C40B50/06 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q2521/101 ,  C12Q2525/191 ,  C12Q2563/179 ,  C12Q2525/151 ,  C12Q2525/185 ,  C12Q2525/204 ,  C12Q2531/131 ,  C12Q2535/138

Abstract: Aspects of the present invention are drawn to methods and compositions for the genetic analysis of regions of interest (ROI) from one or more starting polynucleotide sample(s). In certain aspects, adapter tagged polynucleotide fragments from a plurality of initial polynucleotide samples are pooled, circularized and amplified to produce an immortalized library. Multiple ROI's from this immortalized library are amplified (e.g., in independent iPCR reactions) to generate amplicons, and, in some embodiments, pooled to form a pooled ROI amplicon sample. In certain embodiments, the amplicons for each ROI amplicon in the pooled ROI amplicon sample are present at known molar or mass ratios. The pooled ROI amplicon sample can be analyzed/processed as desired, e.g., sequenced using next generation sequencing technology.

Abstract translation: 本发明的方面涉及用于从一个或多个起始多核苷酸样品进行的感兴趣区域（ROI）的遗传分析的方法和组合物。 在某些方面，来自多个初始多核苷酸样品的衔接子标记的多核苷酸片段被合并，环化并扩增以产生永生化文库。 来自该永生化文库的多个ROI被扩增（例如，在独立的iPCR反应中）以产生扩增子，并且在一些实施方案中合并形成合并的ROI扩增子样品。 在某些实施方案中，合并的ROI扩增子样品中的每个ROI扩增子的扩增子以已知的质量比与质量比存在。 可以根据需要对合并的ROI扩增子样品进行分析/处理，例如使用下一代测序技术测序。

公开(公告)号：WO2013124743A1

公开(公告)日：2013-08-29

申请号：PCT/IB2013/000824

申请日：2013-02-20

Applicant: POPULATION GENETICS TECHNOLOGIES LTD.

Inventor： OSBORNE, Robert ,  SLATTER, Andrew ,  CASBON, James

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6853 ,  C12Q2525/185 ,  C12Q2525/301 ,  C12Q2535/122 ,  C12Q2525/155 ,  C12Q2525/191

Abstract: Aspects of the present invention are drawn to processes for moving a region of interest in a polynucleotide from a first position to a second position with regard to a domain within the polynucleotide. In certain embodiments, the method results in moving a region of interest into functional proximity to specific domain elements present in the polynucleotide (e.g., primer sites and/or MID). Compositions, kits and systems that find use in carrying out the reflex processes described herein are also provided.

Abstract translation: 本发明的方面涉及将多核苷酸中的感兴趣区域从关于多核苷酸内的结构域的第一位置移至第二位置的方法。 在某些实施方案中，该方法导致将感兴趣的区域移动到存在于多核苷酸（例如，引物位点和/或MID）中的特定结构域元件的功能接近。 还提供了用于实施本文所述的反射过程的组合物，试剂盒和系统。

公开(公告)号：WO2011101744A3

公开(公告)日：2011-08-25

申请号：PCT/IB2011/000573

申请日：2011-02-14

Applicant: POPULATION GENETICS TECHNOLOGIES LTD. ,  OSBORNE, Robert ,  SLATTER, Andrew

Inventor： OSBORNE, Robert ,  SLATTER, Andrew

IPC: C12Q1/68

Abstract: Aspects of the present invention are drawn to methods and compositions for the genetic analysis of regions of interest from one or more starting polynucleotide sample, e.g., a multiplexed polynucleotide sample. In certain aspects, a polynucleotide sample comprising one or more region of interest (ROI) is subjected to independent amplification reactions for specific sub-regions within the ROI(s). The amount/concentration of the product from each sub-region amplification reaction is determined followed by producing a normalized sample based on the determined amount/concentration that is suitable for further analyses (e.g., sequencing).

公开(公告)号：WO2011021102A2

公开(公告)日：2011-02-24

申请号：PCT/IB2010/002243

申请日：2010-08-13

Applicant: POPULATION GENETICS TECHNOLOGIES LTD ,  BRENNER, Sydney ,  MIKAWA, Gi ,  OSBORNE, Robert ,  SLATTER, Andrew

Inventor： BRENNER, Sydney ,  MIKAWA, Gi ,  OSBORNE, Robert ,  SLATTER, Andrew

IPC: C12N15/10

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q2563/179 ,  C12Q2537/143 ,  C12Q2535/122 ,  C12Q2525/161 ,  C12Q2525/155 ,  C12Q2525/143

Abstract: Aspects of the present invention are drawn to processes for moving a region of interest in a polynucleotide from a first position to a second position with regard to a domain within the polynucleotide, also referred to as a "reflex method". In certain embodiments, the reflex method results in moving a region of interest into functional proximity to specific domain elements present in the polynucleotide (e.g., primer sites and/or MID). Compositions, kits and systems that find use in carrying out the reflex processes described herein are also provided.

Abstract translation: 本发明的方面涉及将多核苷酸中的感兴趣区域从关于多核苷酸内的结构域的第一位置移动至第二位置的方法，也被称为“反射方法”。 在某些实施方案中，反射方法导致将感兴趣区域移动到存在于多核苷酸（例如，引物位点和/或MID）中的特定结构域元件的功能接近。 还提供了用于实施本文所述的反射过程的组合物，试剂盒和系统。

公开(公告)号：WO2010133972A1

公开(公告)日：2010-11-25

申请号：PCT/IB2010/001392

申请日：2010-05-21

Applicant: POPULATION GENETICS TECHNOLOGIES LTD ,  MUSGRAVE-BROWN, Esther ,  MIKAWA, Gi ,  OSBORNE, Robert ,  SLATTER, Andrew

Inventor： MUSGRAVE-BROWN, Esther ,  MIKAWA, Gi ,  OSBORNE, Robert ,  SLATTER, Andrew

IPC: C12Q1/68

CPC classification number: C12Q1/6809 ,  C12Q2531/107 ,  C12Q2525/191 ,  C12Q2525/155

Abstract: The present invention provides methods and compositions for amplifying and sorting adapter tagged nucleic acid fragments using selective primer extension. Immortalized pooled polynucleotide samples and method of producing the same are also provided.

Abstract translation: 本发明提供了使用选择性引物延伸来扩增和分选衔接子标记的核酸片段的方法和组合物。 还提供了永生化合并的多核苷酸样品及其制备方法。