公开(公告)号：WO2022226316A1

公开(公告)日：2022-10-27

申请号：PCT/US2022/025965

申请日：2022-04-22

Applicant: PRECISION BIOSCIENCES, INC. ,  NORTH CAROLINA STATE UNIVERSITY

Inventor： DEWEY, Ralph ,  JANTZ, Derek ,  SMITH, James Jefferson ,  TOMBERLIN, Ginger H. ,  MORRIS, John ,  MATTHIADIS, Anna ,  SHOOP, Wendy

IPC: C12N9/22 ,  C12N15/82

Abstract: Disclosed herein are recombinant meganucleases engineered to bind and cleave a recognition sequence in the mitochondrial DNA (mtDNA) of a eukaryotic cell, such as a plant cell. The disclosure further relates to the use of such recombinant meganucleases in methods for producing genetically-modified eukaryotic cells, and to a population of genetically-modified eukaryotic cells wherein the mtDNA has been having modified or edited.

公开(公告)号：WO2016046288A1

公开(公告)日：2016-03-31

申请号：PCT/EP2015/071919

申请日：2015-09-23

Applicant: PHILIP MORRIS PRODUCTS S.A ,  NORTH CAROLINA STATE UNIVERSITY

Inventor： LU, Jianli ,  LEWIS, Ramsey, S. ,  DEWEY, Ralph ,  BOVET, Lucien

IPC: C12N15/82 ,  A24B15/24 ,  A01H5/12

CPC classification number: A24B15/245 ,  A01H5/12 ,  A24B15/24 ,  C12N9/0044 ,  C12N15/00 ,  C12N15/8243 ,  C12Y107/00 ,  C12Y107/01001 ,  C12Y107/01002 ,  C12Y107/01003

Abstract: Provided are modified tobacco plants having reduced nitrate levels and tobacco products generated from the modified tobacco plants having reduced tobacco specific nitrosamines (TSNAs). Also provided are methods of reducing TSNAs in tobacco products by altering the gene expression of the nitrate assimilation pathway

Abstract translation: 提供具有降低的硝酸盐水平的改性烟草植物和由具有降低的烟草特异性亚硝胺（TSNA））的改性烟草植物产生的烟草产品。 还提供了通过改变硝酸盐同化途径的基因表达来减少烟草制品中的TSNA的方法