公开(公告)号：WO2022212643A2

公开(公告)日：2022-10-06

申请号：PCT/US2022/022742

申请日：2022-03-31

申请人： QCDX LLC

发明人： TAFAS, Triantafyllos, P. ,  KEILICH, Spencer, Ryan

IPC分类号： G01N1/00 ,  G01N21/00 ,  G01N33/48 ,  G01N33/483 ,  G01N1/30 ,  C12Q1/68 ,  G01N33/533

摘要： The present invention provides methods and systems for analyzing a biological sample. The methods of the present invention can utilize a labeling moiety and a cleavage moiety. The labeling moiety can comprise a binding moiety that is coupled to a detectable tag via a polynucleotide linker. The cleavage moiety can form a complex with the polynucleotide linker. In some cases, after formation of the complex, the cleavage moiety can effect cleavage of the polynucleotide linker, to release the detectable tag from the binding moiety. The release of the detectable tag can allow one or more additional imaging of the biological sample with a different labeling moiety that comprises a different binding moiety but the same labeling moiety.