公开(公告)号：WO2022155549A1

公开(公告)日：2022-07-21

申请号：PCT/US2022/012639

申请日：2022-01-15

Applicant: PURDUE RESEARCH FOUNDATION ,  RAYTHEON BBN TECHNOLOGIES, CORP.

Inventor： HORVATH, Dylan ,  MAN, Charlie ,  LOWE, Andrew ,  DEMPSTER, David ,  ADLER, Aaron ,  BARTLEY, Bryan ,  GAVIN, Mike ,  SEVILLE, Jordan ,  MCCHESNEY, Darby ,  LADUCA, Frank M. ,  WANG, Jiangshan ,  DEXTRE, Andres ,  VERMA, Mohit

IPC: C12Q1/6848

Abstract: The present disclosure is drawn to loop-mediated isothermal amplification (LAMP) reaction assemblies including a substantially hygroscopic agent free LAMP reagent mixture in combination with a solid-phase reaction medium. The present disclosure also includes systems for a chromatic LAMP analysis including a substantially non-reactive solid phase reaction medium, and a non-interfering reagent mixture. The present disclosure also includes solid phase LAMP reaction mediums comprising a substrate, an adhesive layer disposed on the substrate, a reaction layer disposed on the adhesive layer, and a spreading layer disposed on the reaction layer. The present disclosure also includes methods of testing for a presence of a target nucleotide sequence including providing a biological sample, and dispensing the sample into a test environment having a solid phase reaction medium in combination with a LAMP reagent mixture and a pH sensitive dye.

公开(公告)号：WO2022155552A2

公开(公告)日：2022-07-21

申请号：PCT/US2022/012642

申请日：2022-01-15

Applicant: RAYTHEON BBN TECHNOLOGIES, CORP. ,  PURDUE RESEARCH FOUNDATION

Inventor： HORVATH, Dylan ,  MAN, Charlie ,  LOWE, Andrew ,  DEMPSTER, David ,  JAIN, Parth ,  ADLER, Aaron ,  BARTLEY, Bryan ,  DRYER, Paul ,  QUINN, Timothy ,  BAR-COHEN, Avram ,  GAVIN, Mike ,  SEVILLE, Jordan ,  MCCHESNEY, Darby ,  LADUCA, Frank M. ,  VERMA, Mohit

IPC: B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/043 ,  B01L2300/0825 ,  B01L2300/1805 ,  B01L3/5023 ,  B01L7/52 ,  B01L9/527

Abstract: A liquid biological sample test cartridge is disclosed. The cartridge can include a tray. The cartridge can also include a chemical reaction pad supported by the tray. The cartridge can further include a chemical reaction pad cover disposed over the chemical reaction pad and coupled to the tray. The chemical reaction pad cover can have a sample opening to facilitate depositing a liquid biological sample at a predetermined location on the chemical reaction pad. In addition, the cartridge can include an outer cover operable to at least partially form an enclosure about the chemical reaction pad.