公开(公告)号：WO2011070402A1

公开(公告)日：2011-06-16

申请号：PCT/IB2009/055707

申请日：2009-12-11

申请人： SAY, Ridvan

发明人： SAY, Ridvan ,  ERSOZ, Arzu ,  HUR, Deniz ,  YILMAZ, Filiz ,  DENIZLI, Adil ,  OZCAN, Ayca Atilir ,  DILTEMIZ, Sibel Emir ,  YAZAR, Suzan ,  BICEN, Ozlem ,  BUYUKTIRYAKI, Sibel ,  KECILI, Rustem ,  SAKA, Guner ,  FINDIK, Tugba

IPC分类号： C07D209/20 ,  C07D213/06

CPC分类号： G01N33/582 ,  A61K47/643 ,  A61K47/6835 ,  A61K47/6873 ,  A61K49/0065 ,  A61K49/0067 ,  A61K49/1833 ,  A61K49/1848 ,  A61K49/1866 ,  G01N2458/40

摘要： This invention is related to preparation of photosensitive ruthenium based aminoacid monomers and oligomers, aminoacid monomer-protein cross- linking using photo sensitat ion and conjugation on micro and nano-structures by ruthenium-chelate based monomers. Its vast range biotechnolgy applications of multifunctional, biocompatible, stabilE and specific micro and nanobio-conjugates, which will stand-alone or simultaneously enable (i) both purification and determination, (ii) both targeting and imaging and theranostics and (iii) catalysis and determination. The construction and method of preparation is applicable to silica materials, superparamagnetic particles, QDs, CNTs, Ag/ Au nanoparticles and Au surfaces and polymeric materials. The photosensitive aminoacid monomer linkers can react via chemically and biocompatible to a lot of different micro and nano-surface and then to the protein when they act as a single-step cross-linking reaction using irradiation. The photosensitive conjugation based on click biochemistry can be carried out at mild conditions, independent of pH and temperature, without affecting conformation and function of protein.

摘要翻译： 本发明涉及基于钌螯合物的单体制备感光钌基氨基酸单体和低聚物，使用光敏化剂的氨基酸单体 - 蛋白质交联和在微纳米结构上的缀合。 其广泛的多功能，生物相容性，稳定性和特异性微纳米偶联物的生物技术应用，其将独立或同时使得（i）纯化和测定，（ii）靶向和成像和分析和（iii）催化和 决心。 其制备方法适用于二氧化硅材料，超顺磁性颗粒，量子点，碳纳米管，Ag / Au纳米颗粒和Au表面和聚合材料。 光敏氨基酸单体接头可以通过化学和生物相容性反应到大量不同的微观和纳米表面，然后当它们作为使用照射的单步交联反应时，与蛋白反应。 基于点击生物化学的感光共轭可以在温和条件下进行，与pH和温度无关，不影响蛋白质的构象和功能。