公开(公告)号：WO2011163490A3

公开(公告)日：2012-11-08

申请号：PCT/US2011041652

申请日：2011-06-23

Applicant: SMITH CORP A O ,  YAN SONG ,  ZHANG JUNJIE

Inventor： YAN SONG ,  ZHANG JUNJIE

IPC: F24H4/04 ,  F24D19/00

CPC classification number: F24H4/04 ,  F24D19/0092 ,  F24D2200/123 ,  F24H9/1809 ,  F28D7/024 ,  F28D7/026 ,  F28F1/003

Abstract: A heat pump water heater includes a compressor, a water storage tank, a heat exchanger, an expansion device, and an evaporator. The water storage tank has an exterior surface and an interior volume. The heat exchanger includes an external section and an internal section. The heat exchanger is secured to the water storage tank such that the exterior section is in contact with the exterior surface in a heat exchange relationship and the internal section is positioned within the interior volume and is configured to exchange heat with water in the water storage tank. The compressor, the heat exchanger, the expansion device, and the evaporator are connected in series. The external section is configured to receive refrigerant from the compressor, the internal section is configured to supply refrigerant to the expansion device, and the external section is connected in series with the internal section.

Abstract translation: 热泵热水器包括压缩机，储水箱，热交换器，膨胀装置和蒸发器。 储水箱具有外表面和内部容积。 热交换器包括外部部分和内部部分。 热交换器被固定到储水箱，使得外部部分以热交换关系与外部表面接触，并且内部部分位于内部容积内并且构造成与储水箱中的水进行热交换 。 压缩机，热交换器，膨胀装置和蒸发器串联连接。 外部部分被配置为从压缩机接收制冷剂，内部部分被配置为向膨胀装置供应制冷剂，并且外部部分与内部部分串联。

公开(公告)号：WO2007137144A3

公开(公告)日：2008-01-17

申请号：PCT/US2007069194

申请日：2007-05-17

Applicant: UNIV NEW JERSEY MED ,  INOUYE MASAYORI ,  ZHANG JUNJIE ,  SUZUKI MOTOO

Inventor： INOUYE MASAYORI ,  ZHANG JUNJIE ,  SUZUKI MOTOO

IPC: C12P1/00 ,  C12N1/00

CPC classification number: C12N15/70

Abstract: The present invention describes a single-protein production (SPP) system in living E. coli cells that exploits the unique properties of an rnRNA interferase, for example, MazF, a bacterial toxin that is a single stranded RNA- and ACA-specific endoribonuclease, which efficiently and selectively degrades all cellular mRNAs in vivo, resulting in a precipitous drop in total protein synthesis. Concomitant expression of MazF and a target gene engineered to encode an ACA-less mRNA results in sustained and high-level (up to 90%) target expression in the virtual absence of background cellular protein synthesis. Remarkably, target synthesis continues for at least 4 days, indicating that cells retain transcriptional and translational competence despite their growth arrest. SPP technology works well for yeast and human proteins, even a bacterial integral membrane protein. This novel system enables unparalleled signal to noise ratios that should dramatically simplify structural and functional studies of previously intractable but biologically important proteins. The present invention also provides an optimized condensed single protein production system.

Abstract translation: 本发明描述了利用rnRNA干扰酶的独特性质的活的大肠杆菌细胞中的单蛋白质生产（SPP）系统，例如MazF，细菌毒素，其是单链RNA-和ACA特异性内切核糖核酸酶， 其有效和选择性地降解体内所有细胞mRNA，导致总蛋白质合成的急剧下降。 MazF和旨在编码无ACA mRNA的靶基因的伴随表达导致在虚拟无背景细胞蛋白质合成中的持续和高水平（高达90％）的靶表达。 值得注意的是，目标合成持续至少4天，表明细胞保留转录和翻译能力，尽管其生长停滞。 SPP技术适用于酵母和人类蛋白质，甚至细菌整合膜蛋白。 这种新颖的系统能够实现无与伦比的信噪比，这将显着简化以前难以处理但生物重要的蛋白质的结构和功能研究。 本发明还提供优化的冷凝单蛋白生产系统。

公开(公告)号：WO2006055292A3

公开(公告)日：2006-08-03

申请号：PCT/US2005040107

申请日：2005-11-04

Applicant: UNIV NEW JERSEY MED ,  MASAYORI INOUYE ,  ZHANG JUNJIE ,  SUZUKI MOTOO

Inventor： MASAYORI INOUYE ,  ZHANG JUNJIE ,  SUZUKI MOTOO

IPC: C12N5/10 ,  C12P21/00

CPC classification number: C12P21/02 ,  C12N9/22

Abstract: The present invention describes a single-protein production (SPP) system in living E. coli cells that exploits the unique properties of an mRNA interferase, for example, MazF, a bacterial toxin that is a single stranded RNA- and ACA-specific endoribonuclease, which efficiently and selectively degrades all cellular mRNAs in vivo, resulting in a precipitous drop in total protein synthesis. Concomitant expression of MazF and a target gene engineered to encode an ACA-less mRNA results in sustained and high-level (up to 90%) target expression in the virtual absence of background cellular protein synthesis. Remarkably, target synthesis continues for at least 4 days, indicating that cells retain transcriptional and translational competence despite their growth arrest. SPP technology works well for yeast and human proteins, even a bacterial integral membrane protein. This novel system enables unparalleled signal to noise ratios that should dramatically simplify structural and functional studies of previously intractable but biologically important proteins.

Abstract translation: 本发明描述了利用mRNA干扰酶的独特性质的活的大肠杆菌细胞中的单蛋白质生产（SPP）系统，例如MazF，单链RNA-和ACA特异性内切核糖核酸酶的细菌毒素， 其有效和选择性地降解体内所有细胞mRNA，导致总蛋白质合成的急剧下降。 MazF和旨在编码无ACA mRNA的靶基因的伴随表达导致在虚拟无背景细胞蛋白质合成中的持续和高水平（高达90％）的靶表达。 值得注意的是，目标合成持续至少4天，表明细胞保留转录和翻译能力，尽管其生长停滞。 SPP技术适用于酵母和人类蛋白质，甚至细菌整合膜蛋白。 这种新颖的系统能够实现无与伦比的信噪比，这将显着简化以前难以处理但生物重要的蛋白质的结构和功能研究。