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公开(公告)号：WO2017135895A1
公开(公告)日：2017-08-10
申请号：PCT/SG2017/050046
申请日：2017-02-02
发明人： JI, Lianghui , LIU, Yanbin , KOH, Chong Mei John , YAP, Sihui Amy
IPC分类号： C12N15/80 , C12N15/113
摘要： The present invention relates to the field of molecular biology and more particularly to promoters useful for metabolic engineering in yeast or fungi for the production of biobased chemicals with broad applications. Intron-containing promoters with strong activity during oil- accumulation stages are particularly useful for genetic engineering in yeast and fungi, particularly Rhodosporidium or Rhodotorula genera. Such promoter are capable of driving strong expression of RNA or proteins in species of the Rhodosporidium or Rhodotorula genera.
摘要翻译： 本发明涉及分子生物学领域，更具体地涉及用于在酵母或真菌中进行代谢工程的促进剂，用于生产具有广泛应用的生物基化学品。 在油积累阶段具有强活性的含内含子的启动子特别适用于酵母和真菌中的基因工程，特别是红酵母属或红酵母属。 这种启动子能够驱动红色孢子菌属或红酵母属的RNA或蛋白质的强表达。
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2.发明申请METHODS FOR EFFICIENT PRODUCTION OF POLYUNSATURATED FATTY ACIDS (PUFA) IN RHODOSPORIDIUM AND RHODOTORULA SPECIES 审中-公开高效液相色谱法制备多糖脂肪酸（PUFA）在RHODOSPORIDIUM和RHODOTORULA物种中的应用
公开(公告)号：WO2016039685A1
公开(公告)日：2016-03-17
申请号：PCT/SG2015/050273
申请日：2015-08-21
发明人： LIU, Yanbin , KOH, Chong Mei , JI, Lianghui
CPC分类号： C12P7/6427 , C12N9/0006 , C12N9/0008 , C12N9/0071 , C12N9/1025 , C12N9/1029 , C12N9/88 , C12N9/90 , C12N9/93 , C12N15/52 , C12N15/81 , C12P7/6463 , C12Y101/0104 , C12Y102/01003 , C12Y102/01012 , C12Y114/19 , C12Y114/19001 , C12Y114/19003 , C12Y114/19006 , C12Y203/0102 , C12Y203/03008 , C12Y401/01001 , C12Y402/01011 , C12Y502/01008 , C12Y602/01003 , C12Y604/01002
摘要： The present invention relates to the field of fungal biotechnology, more particularly to genetic engineering methods for the production of polyunsaturated fatty acids (PUFA) in fungal hosts selected from Rhodosporidium and Rhodotorula genera. The present invention further relates to a modified fungal host cell having reduced native aldehyde dehydrogenase (ALD l) enzyme activity, and methods for producing omega-3 and omega-6 fatty acids and triacylglycerides, by growing said fungal host cell under suitable conditions.
摘要翻译： 本发明涉及真菌生物技术领域，更具体地涉及在选自Rhodosporidium和Rhodotorula属的真菌宿主中生产多不饱和脂肪酸（PUFA）的基因工程方法。 本发明还涉及具有降低的天然醛脱氢酶（ALD1）酶活性的修饰的真菌宿主细胞，以及通过在合适的条件下生长所述真菌宿主细胞来生产ω-3和ω-6脂肪酸和三酰基甘油酯的方法。
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公开(公告)号：WO2018147799A1
公开(公告)日：2018-08-16
申请号：PCT/SG2018/050045
申请日：2018-01-29
发明人： JI, Lianghui , LIU, Yanbin , KOH, Chong Mei John , YAP, Sihui Amy , NGOH, Si Te
摘要： The present invention relates to the field of fungal production fatty alcohols. More specifically, the present invention relates to genetically modified host cells, nucleic acid constructs and culture medium for the production of fatty alcohols in Rhodosporidium .
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4.发明申请D-AMINO ACID-INDUCIBLE GENE EXPRESSION SYSTEM FOR RHODOSPORIDIUM AND RHODOTORULA 审中-公开D-氨基酸诱导的基因表达系统和RHODOTORULA
公开(公告)号：WO2016159887A1
公开(公告)日：2016-10-06
申请号：PCT/SG2016/050161
申请日：2016-03-31
发明人： JI, Lianghui , LIU, Yanbin , KOH, Chong Mei John
IPC分类号： C12N15/81
CPC分类号： C12N15/815 , C12N1/16 , C12N15/80 , C12N15/81 , C12N2830/36 , C12N2840/002
摘要： The present invention relates to the field of fungal biotechnology, more particularly to a strong inducible gene expression system in fungal species, such as a species of the Rhodosporidium genus or the Rhodotorula genus.
摘要翻译： 本发明涉及真菌生物技术领域，更具体地涉及真菌物种中的强诱导型基因表达系统，例如Rhodosporidium属或Rhodotorula属的物种。
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5.发明申请METHODS FOR TUNING CAROTENOID PRODUCTION LEVELS AND COMPOSITIONS IN RHODOSPORIDIUM AND RHODOTORULA GENERA 审中-公开用于调节牙龈卟啉单胞菌和罗丹霉属发生中的碳酸盐生产水平和组成的方法
公开(公告)号：WO2016099401A1
公开(公告)日：2016-06-23
申请号：PCT/SG2015/050491
申请日：2015-12-14
发明人： LIU, Yanbin , KOH, Chong Mei , JI, Lianghui
CPC分类号： C07K14/37 , C12N9/0008 , C12N15/113 , C12N15/80 , C12P21/02 , C12P23/00
摘要： The present invention relates to the field of fungal biotechnology, more particularly to genetic engineering methods for the production of carotenoids in fungal hosts selected from Rhodospordium and Rhodotorula genera.
摘要翻译： 本发明涉及真菌生物技术领域，更具体地涉及用于在选自Rhodosporium和Rhodotorula属的真菌宿主中生产类胡萝卜素的基因工程方法。
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