公开(公告)号：WO2017181044A1

公开(公告)日：2017-10-19

申请号：PCT/US2017/027679

申请日：2017-04-14

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： WALLER, Laura ,  PHILLIPS, Zachary ,  CHEN, Michael

IPC分类号： G02B21/36 ,  G02B21/06 ,  G02B5/20 ,  G06T7/60

CPC分类号： G02B5/201 ,  G02B21/14 ,  G02B21/365 ,  H04N5/23229 ,  H04N5/23264 ,  H04N9/00 ,  H04N9/07 ,  H04N2209/043 ,  H04N2209/044

摘要： Systems and methods are disclosed for recovering both phase and amplitude of an arbitrary sample in an optical microscope from a single image, using patterned partially coherent illumination. This is realized through the use of a encoded light source which embeds several different illumination patterns into color channels. The sample is modulated by each illumination wavelength separately and independently of each other, but all of the channels are sensed by the imaging device in a single step. This color image contains information about the phase and amplitude of a sample encoded in each channel, and can be used to recover both amplitude and phase from this single image, at the incoherent resolution limit. Further, extensions of this method are shown which allow the same recovery of a sample whilst it is moving during a single exposure using a motion deblurring algorithm.

摘要翻译： 公开了用于使用图案化的部分相干照明从单个图像恢复光学显微镜中的任意样本的相位和振幅的系统和方法。 这是通过使用将几种不同照明图案嵌入彩色通道的编码光源来实现的。 每个照明波长分别独立地调制样品，但所有通道均由成像设备一步感应。 此彩色图像包含有关每个通道中编码的样本的相位和幅度的信息，并且可用于在非相干分辨率极限下从该单个图像中恢复幅度和相位。 此外，还展示了这种方法的扩展，它允许在使用运动去模糊算法在单次曝光期间移动的同时恢复样品。