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    COMPOSITIONS AND METHODS FOR THE PRODUCTION OF HYDROCARBONS, HYDROGEN AND CARBON MONOXIDE USING ENGINEERED AZOTOBACTER STRAINS
    1.
    发明申请
    COMPOSITIONS AND METHODS FOR THE PRODUCTION OF HYDROCARBONS, HYDROGEN AND CARBON MONOXIDE USING ENGINEERED AZOTOBACTER STRAINS 审中-公开
    使用工程化的缩氮杆菌菌株生产烃，氢和一氧化碳的组合物和方法

    公开(公告)号：WO2017091510A1

    公开(公告)日：2017-06-01

    申请号：PCT/US2016/063123

    申请日：2016-11-21

    申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    发明人： RIBBE, Markus W. ,  HU, Yilin ,  REBELEIN, Johannes ,  LEE, Chi

    IPC分类号： C12N9/02 ,  C12P5/02

    CPC分类号： C12P5/02 ,  C12R1/065 ,  Y02E50/343

    摘要： In alternative embodiments, provided are genetically or recombinantly engineered nitrogen-fixing, nitrogenase-expressing bacteria capable of enzymatically synthesizing hydrocarbons and generating hydrogen and carbon monoxide, and for carbon dioxide and/or carbon monoxide recycling, and compositions (e.g., bioreactors and devices) for using them, and methods for making and using them. In alternative embodiments, the genetically or recombinantly engineered nitrogen-fixing, nitrogenase expressing bacteria used to practice embodiments provided herein include nitrogen-fixing diazotrophs such as nitrogen-fixing bacteria of the family Pseudomonadaceae , or the genus Azotobacter , including Azotobacter vinelandii , for the whole cell synthesis of hydrocarbons and generating hydrogen and carbon monoxide, and for the recycling of carbon dioxide and/or carbon monoxide.

    摘要翻译： 在备选实施方案中，提供了能够酶促合成烃并产生氢和一氧化碳并且用于二氧化碳和/或一氧化碳再循环的遗传或重组工程化的固氮，表达固氮酶的细菌，以及 用于使用它们的组合物（例如生物反应器和装置），以及制造和使用它们的方法。 在替代实施方案中，用于实施本文提供的实施方案的遗传或重组工程化固氮，表达固氮的细菌包括固氮固氮菌，如假单胞菌科的固氮菌或固氮菌属，包括Azotobacter vinelandii，用于全部细胞合成碳氢化合物并产生氢和一氧化碳，以及用于二氧化碳和/或一氧化碳的再循环。

    RECOMBINANTLY ENGINEERED DIAZOTROPHS FOR WHOLE CELL HYDROCARBON PRODUCTION AND METHODS FOR MAKING AND USING THEM
    2.
    发明申请
    RECOMBINANTLY ENGINEERED DIAZOTROPHS FOR WHOLE CELL HYDROCARBON PRODUCTION AND METHODS FOR MAKING AND USING THEM 审中-公开
    用于全细胞碳氢化合物生产的重组工程DIAZOTROPHS及其制备和使用方法

    公开(公告)号：WO2016100727A1

    公开(公告)日：2016-06-23

    申请号：PCT/US2015/066481

    申请日：2015-12-17

    申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    发明人： RIBBE, Markus Walter ,  HU, Yilin ,  REBELEIN, Johannes

    IPC分类号： C12Q1/04

    CPC分类号： C12N1/20 ,  C07K14/21 ,  C12F3/04 ,  C12M23/08 ,  C12P3/00 ,  C12P5/02 ,  C12P5/023 ,  C12P5/026 ,  C12Q1/04 ,  Y02E50/343

    摘要： In alternative embodiments, provided are genetically or recombinantly engineered nitrogen-fixing, nitrogenase expressing bacteria capable of enzymatically synthesizing hydrocarbons, and methods for making and using them. In alternative embodiments, provided are genetically or recombinantly engineered nitrogen-fixing, nitrogenase expressing bacteria including nitrogen-fixing diazotrophs such as nitrogen-fixing bacteria of the family Pseudomonadaceae , or the genus Azotobacter , for the whole cell synthesis of hydrocarbons and carbon-carbon bonds. In alternative embodiments, nitrogen-fixing, nitrogenase-expressing bacteria used to practice the invention are genetically or recombinantly engineered to express an exogenous nitrogenase express more endogenous nitrogenase or have increased nitrogenase, activity. In alternative embodiments, nitrogen-fixing, nitrogenase-expressing bacteria used to practice the invention are genetically or recombinantly engineered to lack or have decreased molybdenum transporter activity. In alternative embodiments, provided are culture systems, fermenters and bioreactors using nitrogen-fixing, nitrogenase-expressing bacteria for enzymatically synthesizing hydrocarbons.

    摘要翻译： 在替代实施方案中，提供了能够酶促合成烃的遗传或重组工程化的氮固定，表达氮的酶的细菌，以及制备和使用它们的方法。 在替代实施方案中，提供了遗传或重组工程化的氮固定，含氮酶表达细菌，包括固氮重氮营养物，例如假单胞菌科的固氮菌或固氮菌属，用于碳氢化合物和碳 - 碳键的全部细胞合成 。 在替代实施方案中，用于实施本发明的固氮，含氮酶的细菌进行遗传或重组工程改造，以表达外源氮酶表达更多的内源性氮酶或具有增加的氮酶活性。 在替代实施方案中，用于实施本发明的固氮，含氮酶的细菌被遗传或重组工程化以缺乏或具有降低的钼转运蛋白活性。 在替代实施方案中，提供了使用氮固定，氮酶表达细菌用于酶合成烃的培养系统，发酵罐和生物反应器。