公开(公告)号：WO1990000191A1

公开(公告)日：1990-01-11

申请号：PCT/US1989002809

申请日：1989-06-27

Applicant: THE SALK INSTITUTE BIOTECHNOLOGY/INDUSTRIAL ...

Inventor： THE SALK INSTITUTE BIOTECHNOLOGY/INDUSTRIAL ... ,  TSCHOPP, Juerg, Friedrich ,  DIXON, Douglas, Brian

IPC: C12N01/00

CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N15/815 ,  C12N2760/20122

Abstract: The present invention provides a method of producing a rabies glycoprotein G by culturing cells of a methylotrophic species of yeast, which have a gene capable of expressing the pre-form of the rabies glycoprotein G in such cells, under conditions such that the gene is transcribed. The preferred species of yeast for carrying out the invention is Pichia pastoris. Mature rabies glycoprotein G is expressed at a high level in methylotrophic yeast cells as an extractable, membrane-associated glycoprotein. Also provided by the invention are DNAs capable of transforming methylotrophic yeast to express rabies pre-glycoprotein Gs, cultures of cells of such a yeast transformed with such DNAs and the novel glycosylated rabies glycoprotein Gs made by the method of the invention. The rabies glycoprotein G provided by the invention is useful as an immunogen in vaccines to protect mammals against infection by rabies virus.

Abstract translation: 本发明提供了通过培养酵母的甲基营养物质的细胞来生产狂犬病糖蛋白G的方法，所述酵母的细胞在具有能够在该细胞中表达狂犬病糖蛋白G的前体形式的基因的条件下， 。 用于实施本发明的优选酵母菌种是巴斯德毕赤酵母。 成熟的狂犬病糖蛋白G在甲基营养酵母细胞中以高水平表达，作为可提取的膜相关糖蛋白。 本发明还提供能够转化甲基营养酵母以表达狂犬病前糖蛋白G的DNA，用这种DNA转化的酵母的细胞培养物和通过本发明的方法制备的新的糖基化狂犬病糖蛋白Gs。 本发明提供的狂犬病糖蛋白G可用作疫苗中的免疫原，以保护哺乳动物免受狂犬病毒感染。