公开(公告)号：WO2006073449A2

公开(公告)日：2006-07-13

申请号：PCT/US2005/014499

申请日：2005-04-27

Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK ,  LIPKIN, W., Ian ,  BRIESE, Thomas ,  PALACIOS, Gustavo ,  JABADO, Omar

Inventor： LIPKIN, W., Ian ,  BRIESE, Thomas ,  PALACIOS, Gustavo ,  JABADO, Omar

IPC: C12Q1/68 ,  C12M1/34

CPC classification number: C12Q1/6837 ,  C12Q2565/549 ,  C12Q2565/125 ,  C12Q2531/113 ,  C12Q2563/149

Abstract: The present invention provides systems and methods for determining the presence or absence of one or more target nucleic acid sequences in a sample. Also provided are kits comprising these systems, and uses of these systems in such applications as determining the presence or absence of at least one target nucleic acid sequence in a sample, detecting microorganism transcripts and host transcripts, differentiating microorganism transcripts from host transcripts, screening blood products, assaying a food product for contamination, assaying a sample for environmental contamination, detecting genetically-modified organisms, biodefense, forensics, and genetic-comparability studies. The present invention further provides a complex that includes a target nucleic acid sequence, a capture nucleic acid, and a reporter nucleic acid.

Abstract translation: 本发明提供用于确定样品中一种或多种靶核酸序列的存在或不存在的系统和方法。 还提供了包括这些系统的试剂盒，以及这些系统在确定样品中是否存在至少一种靶核酸序列的应用中的用途，检测微生物转录物和宿主转录物，将微生物转录物与宿主转录物区分开来，筛选血液 产品，测定食品污染，测定样品的环境污染，检测转基因生物，生物防治，取证和遗传比较研究。 本发明还提供了包含靶核酸序列，捕获核酸和报道核酸的复合物。

公开(公告)号：WO2017049285A1

公开(公告)日：2017-03-23

申请号：PCT/US2016/052481

申请日：2016-09-19

Applicant: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK

Inventor： LIPKIN, Walter, Ian ,  JABADO, Omar ,  BRIESE, Thomas ,  KAPOOR, Amit

IPC: C12Q1/70 ,  C12Q1/68 ,  C12R1/92 ,  C40B40/06 ,  C12P19/34 ,  C12M1/34 ,  G06F19/20 ,  G06F19/10

CPC classification number: C12N15/1006 ,  C12Q1/70 ,  C40B40/06 ,  G06F19/10 ,  G06F19/20 ,  C12Q2535/122

Abstract: The present invention provides novel methods, systems, tools, and kits for the simultaneous detection, identification and/or characterization of all viruses known or suspected to infect vertebrates. The methods, systems, tools, and kits described herein are based upon the virome capture sequencing platform ("VirCapSeq-VERT"), a novel platform developed by the inventors. The invention also provides methods and kits for designing and constructing of the virome capture sequencing platform.

Abstract translation: 本发明提供用于同时检测，鉴定和/或表征已知或怀疑感染脊椎动物的所有病毒的新颖方法，系统，工具和试剂盒。 本文描述的方法，系统，工具和试剂盒基于由发明人开发的新颖平台的病毒捕获测序平台（“VirCapSeq-VERT”）。 本发明还提供了用于设计和构建病毒捕获测序平台的方法和试剂盒。