公开(公告)号：WO2020061251A8

公开(公告)日：2020-03-26

申请号：PCT/US2019/051827

申请日：2019-09-19

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： BRANGWYNNE, Cliff ,  BRACHA, Dan ,  JUMPER, Chanelle ,  ACKERMAN, Paul

IPC: C07K1/36 ,  C07K14/00 ,  C07K14/435 ,  C07K14/47 ,  C12N9/88 ,  C12N11/02

Abstract: Disclosed is a high-throughput method and system for mapping or screening biomolecular interactions, where the method includes providing a plurality of cells, or in some examples purified proteins themselves, expressing a phase separation or aggregation system, including those capable of being controlled by at least one wavelength of light, with the phase separation or aggregation system comprising a target protein possibly fused to a fluorescent protein. The cells are placed in a well, and chemical and/or biological agent are then introduced to the well. The well may then be the irradiated with the wavelength that controls the phase separation or aggregation system, after which the well is irradiated so as to cause the fluorescent proteins or fluorophores to fluoresce, after which the phase separation or aggregation can be quantified based on an amount of fluorescence within a first region and a second region of the sample, the first region containing a condensate and the second region not containing a condensate. Also disclosed is a method utilizing a non-optically controlled phase separation or aggregation system.

公开(公告)号：WO2022159642A2

公开(公告)日：2022-07-28

申请号：PCT/US2022/013213

申请日：2022-01-21

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： BRANGWYNNE, Clifford, P. ,  JUMPER, Chanelle ,  ACKERMAN, Paul ,  BRACHA, Dan

IPC: G01N33/50 ,  G01N33/68 ,  C12N15/85

Abstract: Provided herein are methods of identifying an inhibitory activity of an exogeneous agent that disrupts binding of a target peptide to a biomolecule using phase separation as in cellulo read-out. Provided herein also are kits for the methods, pharmaceutical compositions comprising the agents screened by the methods, methods of preparing the pharmaceutical composition, and methods of treating a disease or condition in a subject in need thereof by administering the pharmaceutical composition.