公开(公告)号：WO2020061251A8

公开(公告)日：2020-03-26

申请号：PCT/US2019/051827

申请日：2019-09-19

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： BRANGWYNNE, Cliff ,  BRACHA, Dan ,  JUMPER, Chanelle ,  ACKERMAN, Paul

IPC: C07K1/36 ,  C07K14/00 ,  C07K14/435 ,  C07K14/47 ,  C12N9/88 ,  C12N11/02

Abstract: Disclosed is a high-throughput method and system for mapping or screening biomolecular interactions, where the method includes providing a plurality of cells, or in some examples purified proteins themselves, expressing a phase separation or aggregation system, including those capable of being controlled by at least one wavelength of light, with the phase separation or aggregation system comprising a target protein possibly fused to a fluorescent protein. The cells are placed in a well, and chemical and/or biological agent are then introduced to the well. The well may then be the irradiated with the wavelength that controls the phase separation or aggregation system, after which the well is irradiated so as to cause the fluorescent proteins or fluorophores to fluoresce, after which the phase separation or aggregation can be quantified based on an amount of fluorescence within a first region and a second region of the sample, the first region containing a condensate and the second region not containing a condensate. Also disclosed is a method utilizing a non-optically controlled phase separation or aggregation system.

公开(公告)号：WO2020014588A1

公开(公告)日：2020-01-16

申请号：PCT/US2019/041564

申请日：2019-07-12

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： BRANGWYNNE, Cliff ,  DRAKE, Victoria ,  SANDERS, David

IPC: A61K38/00 ,  A61K47/48 ,  C07K14/47 ,  C07K19/00 ,  C12N15/09 ,  C12N5/10 ,  C12N15/62

Abstract: A system and method for modulating stress granule assembly, utilizing a protein construct that has a cell penetrating protein fused to one or more proteins that can bind with an NTF2- like domain of a G3BP protein. By configuring the protein construct with an appropriate number of proteins that being with NTF2-like domains, stress granule assembly can be upregulated or downregulated as needed to treat patients.

公开(公告)号：WO2019147611A3

公开(公告)日：2019-08-01

申请号：PCT/US2019/014666

申请日：2019-01-23

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： BRANGWYNNE, Cliff ,  SHIN, Yongdae ,  BRACHA, Dan

IPC: C07K14/00 ,  C07K19/00 ,  C12N11/02 ,  C12Q1/68 ,  G01N21/64 ,  G01N33/536

Abstract: Disclosed is a system and method for the controlled clustering of gene regulatory proteins at the specific target genomic loci. The technology enables formation of liquid-like droplets closely interfaced with genome organization. This technology can be utilized for perturbing endogenous nuclear structures as well as nucleating synthetic membrane-less assemblies. The technology can also be utilized for inducing or detecting mechanical stresses within the genome.

公开(公告)号：WO2019147611A2

公开(公告)日：2019-08-01

申请号：PCT/US2019/014666

申请日：2019-01-23

Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY

Inventor： BRANGWYNNE, Cliff ,  SHIN, Yongdae ,  BRACHA, Dan

IPC: C12N15/85

Abstract: Disclosed is a system and method for the controlled clustering of gene regulatory proteins at the specific target genomic loci. The technology enables formation of liquid-like droplets closely interfaced with genome organization. This technology can be utilized for perturbing endogenous nuclear structures as well as nucleating synthetic membrane-less assemblies. The technology can also be utilized for inducing or detecting mechanical stresses within the genome.