公开(公告)号：WO1997016221A2

公开(公告)日：1997-05-09

申请号：PCT/IB1996001277

申请日：1996-10-30

Applicant: THE UAB RESEARCH FOUNDATION

Inventor： THE UAB RESEARCH FOUNDATION ,  BUCHSBAUM, Donald, J. ,  RABEN, David ,  KHAZAELI, Mohammad, B. ,  STACKHOUSE, Murray ,  CURIEL, David

IPC: A61N00/00

CPC classification number: A61K51/1036 ,  A61K38/00 ,  A61K38/105 ,  A61K38/1796 ,  A61K48/00 ,  A61K51/08 ,  A61K51/088 ,  A61K51/10 ,  A61K2123/00 ,  C07K14/70503 ,  C07K14/82 ,  C07K16/3007 ,  C07K2317/77

Abstract: The present invention provides a method to achieve radioisotopic localization at tumor sites, i.e., a method of enhancing radiolabeled ligand localization to a tumor in an individual in need of such treatment, comprising the steps of: transducing said tumor with a gene encoding a membrane expressed protein unique to said tumor; and administering to said individual a radiolabeled ligand which specifically binds to said protein. The use of gene therapy technology to induce expression of high affinity membrane molecules/receptors can enhance the specificity of radioisotope localization while the use of radioactive isotopes with the ability to deliver radiation damage across several cell diameters will compensate for less than perfect transduction efficiency.

Abstract translation: 本发明提供了一种在肿瘤部位实现放射性同位素定位的方法，即在需要这种治疗的个体中增强放射性标记的配体定位于肿瘤的方法，包括以下步骤：用编码膜表达的基因转导所述肿瘤 所述肿瘤独特的蛋白质; 并向所述个体施用特异性结合所述蛋白质的放射性标记的配体。 使用基因治疗技术诱导高亲和力膜分子/受体的表达可以增强放射性同位素定位的特异性，而使用具有在几个细胞直径上传递辐射损伤的能力的放射性同位素将补偿不到完美的转导效率。

公开(公告)号：WO1998018489A1

公开(公告)日：1998-05-07

申请号：PCT/US1997019911

申请日：1997-10-30

Applicant: THE UAB RESEARCH FOUNDATION

Inventor： THE UAB RESEARCH FOUNDATION ,  BUCHSBAUM, Donald, J. ,  CURIEL, David, T. ,  STACKHOUSE, Murray

IPC: A61K39/395

CPC classification number: C07K16/18 ,  A61K2039/51

Abstract: The present invention provides a method of enhancing the chemosensitivity and radiosensitivity of a neoplastic cell expressing an oncoprotein that stimulates proliferation of the cell, comprising introducing into the cell a nucleic acid molecule encoding an antibody homologue, wherein the antibody homologue is expressed intracellularly and binds to the oncoprotein intracellularly in the endoplasmic reticulum of the cell. The present invention is also directed to a method for enhancing the inhibition of proliferation of a neoplastic cell expressing an oncoprotein that stimulates proliferation of the cell, comprising the steps of: introducing into the cell a nucleic acid molecule encoding an antibody homologue, wherein the antibody homologue is expressed intracellularly and binds to the protein intracellularly; and contacting said cell with an anti-neoplastic agent.

Abstract translation: 本发明提供增强表达刺激细胞增殖的癌蛋白的肿瘤细胞的化学敏感性和放射敏感性的方法，包括将编码抗体同系物的核酸分子导入细胞，其中抗体同系物在细胞内表达并结合 胞内胞内蛋白质细胞内质网。 本发明还涉及一种用于增强表达癌细胞增殖的肿瘤细胞增殖抑制的方法，所述方法包括以下步骤：将编码抗体同系物的核酸分子导入细胞，其中抗体 同源物在细胞内表达并在细胞内结合蛋白; 并使所述细胞与抗肿瘤剂接触。