公开(公告)号：WO2016123692A1

公开(公告)日：2016-08-11

申请号：PCT/CA2016/000031

申请日：2016-02-04

Applicant: THE UNIVERSITY OF BRITISH COLUMBIA

Inventor： HANSEN, Carl Lars Genghis ,  ZAHN, Hans ,  HUFT, Jens ,  VAN LOENHOUT, Marinus Theodorus Johannes ,  LEUNG, Kaston ,  LIN, Bill Kengli ,  KLAUS, Anders ,  APARICIO, Samuel Alves Jana Rodrigues ,  SHAH, Sohrab Prakash

IPC: C40B50/06 ,  B81B1/00 ,  B81B7/04 ,  C12M1/34 ,  C12N15/00 ,  C12P19/34 ,  C12Q1/00 ,  C12Q1/68 ,  C40B20/00 ,  C40B30/00 ,  C40B40/06 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G06F19/10

CPC classification number: C12Q1/6869 ,  B01L3/502761 ,  B01L3/505 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/14 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q2531/119 ,  C12Q2563/149 ,  C12Q2565/629 ,  C12Q2525/191 ,  C12Q2531/113

Abstract: Methods, devices and systems for analyzing precious samples of cells, including single cells are provided. The methods, devices, and systems in various embodiments of the invention are used to assess genomic heterogeneity, which has been recognized as a central feature of many cancers and plays a critical role in disease initiation, progression, and response to treatment. The methods devices and systems are also used to analyze embryonic biopsies for reimplantation genetic diagnosis (PGD). In one embodiment, the devices, systems and methods provided herein allow for the construction of genomic and RNA-seq libraries without a pre- amplification step.

Abstract translation: 提供了分析包括单细胞在内的珍贵细胞样品的方法，装置和系统。 本发明的各种实施方案中的方法，装置和系统用于评估基因组异质性，其被认为是许多癌症的中心特征，并且在疾病起始，进展和对治疗的反应中起关键作用。 方法设备和系统也用于分析胚胎活组织检查用于再植入遗传诊断（PGD）。 在一个实施方案中，本文提供的装置，系统和方法允许在没有预扩增步骤的情况下构建基因组和RNA-seq文库。