公开(公告)号：WO2017173229A1

公开(公告)日：2017-10-05

申请号：PCT/US2017/025305

申请日：2017-03-31

申请人： TUFTS UNIVERSITY

发明人： CAMILLI, Andrew ,  CAIRNS, Lynne ,  YEN, Minmin

IPC分类号： C12R1/91 ,  A01N63/02 ,  C12N7/00

摘要： Compositions and methods are provided for preventing, ameliorating, or treating a disease caused by a species of bacterial genus Vibrio, for example, cholera caused by V. cholerae, the compositions containing two or more strains of lytic bacteriophage that infect and kill Vibrio cells. The bacteriophage are virulent, which replicate intracellularly and lyse and kill the bacteria. Use of two or more strains in a single treatment, as a result of a rate of mutation of the bacteria to simultaneous resistance to all of the bacteriophage to be so low as to be negligible, reduces appearance of phage-resistant bacteria to statistical negligibility. Normal human microbial flora species were not affected. In alternative embodiments of the method and the composition, antibiotic agents or other treatment agents can be administered with a cocktail of the plurality of bacteriophage strains.

摘要翻译： 提供组合物和方法用于预防，改善或治疗由弧菌属细菌种类引起的疾病，例如由霍乱弧菌引起的霍乱，含有两种或更多种溶解菌株的组合物 噬菌体感染和杀死弧菌细胞。 噬菌体是强毒的，它在细胞内复制并裂解并杀死细菌。 由于细菌突变率与同时对所有噬菌体的抗性低至可忽略不计的程度，使得在单一处理中使用两种或更多种菌株，从而减少了抗噬菌体细菌出现统计学上的可忽略性。 正常的人类微生物菌群不受影响。 在该方法和组合物的可选实施方案中，抗生素剂或其他治疗剂可以与多种噬菌体菌株的混合物一起施用。

公开(公告)号：WO2004020609A2

公开(公告)日：2004-03-11

申请号：PCT/US2003/027401

申请日：2003-09-02

申请人： TUFTS UNIVERSITY ,  CAMILLI, Andrew ,  HAVA, David, L.

发明人： CAMILLI, Andrew ,  HAVA, David, L.

IPC分类号： C12N

CPC分类号： C07K14/315 ,  A61K39/00 ,  A61K2039/505

摘要： The present invention relates to recombinant antigenic S. pneumoniae polypeptides and fragments thereof. The invention also relates to methods for using these polypeptides to produce immunological responses and to confer immunological protection to disease caused by members of the genus Streptococcus, at least isolates of the S. pneumoniae genus. The invention further relates to nucleic acid sequences which encode antigenic S. pneumoniae polypeptides and to methods for detecting S. pneumoniae nucleic acids and polypeptides in biological samples. The invention also relates to S. pneumoniae -specific antibodies and methods for detecting such antibodies produced in a host animal.

摘要翻译： 本发明涉及重组抗原性S。 肺炎多肽及其片段。 本发明还涉及使用这些多肽产生免疫应答并赋予由链球菌属成员引起的疾病的免疫保护的方法，所述疾病至少是S的分离物。 肺炎杆菌属。 本发明进一步涉及编码抗原性S的核酸序列。 肺炎多肽和用于检测S的方法。 肺炎球菌核酸和多肽在生物样品中的应用。 本发明还涉及S。 肺炎球菌特异性抗体和检测宿主动物产生的这种抗体的方法。

公开(公告)号：WO2015168600A2

公开(公告)日：2015-11-05

申请号：PCT/US2015/028851

申请日：2015-05-01

申请人： TUFTS UNIVERSITY

发明人： DALIA, Ankur B. ,  CAMILLI, Andrew

IPC分类号： C12N15/85

CPC分类号： C12N15/902

摘要： The present invention includes compositions and methods of co-transformation of naturally competent cells. In one aspect of the invention, a method is included for introducing nucleic acid sequences into one or more naturally competent cells in parallel. In other aspects, a heterogenic pool of co-transformed naturally competent cells and an apparatus for introducing two or more populations of nucleic acid sequences into a population of naturally competent cells in parallel are also included.

摘要翻译： 本发明包括天然感受态细胞共转化的组合物和方法。 在本发明的一个方面，包括用于将核酸序列并入一个或多个天然感受态细胞的方法。 在其他方面，还包括共转化天然感受态细胞的异源池和用于将两个或更多个核酸序列群并入天然感受态细胞群的装置。

公开(公告)号：WO2014031954A1

公开(公告)日：2014-02-27

申请号：PCT/US2013/056383

申请日：2013-08-23

申请人： TUFTS UNIVERSITY

发明人： LAZINSKI, David, W. ,  CAMILLI, Andrew

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12P19/34 ,  C12Q1/6855 ,  C12Q2525/173

摘要： According to some aspects of the invention, provided herein are methods of amplifying nucleic acids using homopolymer-mediated ligation. The methods, in some embodiments, comprise adding a first homopolymer of at least 12 nucleotides to each 3' end of blunt-ended double-stranded nucleic acid containing a target nucleic acid, thereby producing a partially double-stranded nucleic acid.

摘要翻译： 根据本发明的一些方面，本文提供了使用均​​聚物介导的连接扩增核酸的方法。 在一些实施方案中，所述方法包括向含有靶核酸的平端双链核酸的每个3'末端加入至少12个核苷酸的第一均聚物，从而产生部分双链核酸。

公开(公告)号：WO2009049013A9

公开(公告)日：2009-04-16

申请号：PCT/US2008/079292

申请日：2008-10-09

申请人： TUFTS UNIVERSITY ,  CAMILLI, Andrew ,  SCHILD, Stefan ,  NELSON, Eric, Jorge

发明人： CAMILLI, Andrew ,  SCHILD, Stefan ,  NELSON, Eric, Jorge

IPC分类号： A61K39/02 ,  A61P31/04 ,  A61P1/12

摘要： The methods and compositions of the present invention are directed to a vaccine against Vibrio cholerae comprised of V. cholerae outer membrane vesicles (OMVs). Such vaccines are relatively stable, facilitating distribution. Inventive methods generally include administration of a vaccine against Vibrio cholerae by intranasal, intraperitoneal, oral or intragastric routes. Such vaccines confer immunity to the individual, and when administered to pregnant subjects, can be conferred to the offspring of individuals.