公开(公告)号：WO2018183380A1

公开(公告)日：2018-10-04

申请号：PCT/US2018/024636

申请日：2018-03-27

Applicant: TWO PORE GUYS, INC.

Inventor： HENDRICKS, Stephen, P. ,  MORIN, Trevor, J.

IPC: G01N33/50 ,  G01N33/483 ,  G01N27/00 ,  C12Q1/68

Abstract: Disclosed herein are methods and compositions for target polynucleotide identification by using modified nucleotides incorporated into the polynucleotide to facilitate detection via a nanopore device.

公开(公告)号：WO2016126748A1

公开(公告)日：2016-08-11

申请号：PCT/US2016/016235

申请日：2016-02-02

Applicant: TWO PORE GUYS, INC.

Inventor： MORIN, Trevor, J. ,  DUNBAR, William, B. ,  HELLER, Daniel, A. ,  SHROPSHIRE, Tyler

IPC: G01N33/543 ,  G01N33/569 ,  G01N33/574

CPC classification number: C12Q1/37 ,  C12Q1/14 ,  C12Q1/34 ,  C12Q1/527 ,  G01N33/48721

Abstract: Disclosed herein are methods and compositions for electronic detection and/or quantification of enzymes or enzymatic activity in a sample using a pore system.

Abstract translation: 本文公开了用于使用孔系统在样品中电子检测和/或定量酶或酶活性的方法和组合物。

公开(公告)号：WO2017173392A1

公开(公告)日：2017-10-05

申请号：PCT/US2017/025585

申请日：2017-03-31

Applicant: TWO PORE GUYS, INC.

Inventor： HELLER, Daniel, A. ,  DUNBAR, William, B. ,  MORIN, Trevor, J. ,  SHROPSHIRE, Tyler

IPC: G01N33/487 ,  G01N33/483 ,  G01N27/66 ,  G01N27/447 ,  C12Q1/68

Abstract: Disclosed herein are methods and compositions for detecting a target DNA sequence from a sample that does not require sample purification or amplification. The method uses fragmentation, sequence-specific binding or ligation of probes, and payload molecules for selective detection of the target-sequence using a nanopore sensor.

Abstract translation: 本文公开了用于从不需要样品纯化或扩增的样品中检测靶DNA序列的方法和组合物。 该方法使用探针的片段化，序列特异性结合或连接以及使用纳米孔传感器选择性检测靶序列的有效负载分子。

公开(公告)号：WO2017091724A1

公开(公告)日：2017-06-01

申请号：PCT/US2016/063597

申请日：2016-11-23

Applicant: TWO PORE GUYS, INC.

Inventor： MORIN, Trevor, J. ,  HELLER, Daniel, A. ,  DUNBAR, William, B. ,  SHROPSHIRE, Tyler

IPC: G01N27/447 ,  G01N33/487

CPC classification number: G01N33/5308 ,  B82Y15/00 ,  C12Q1/68 ,  G01N27/447 ,  G01N30/96 ,  G01N33/48721 ,  G01N33/9453 ,  G01N2030/8827 ,  C12Q2565/631

Abstract: The present invention is directed to a target molecule modified to facilitate detection in a nanopore deice. The present invention further relates to a method of detecting such a modified target molecule using a nanopore device. It also disclose a method of using such a modified target molecule for tracking and verification of pharmaceutical, chemical or biological products and for measuring various conditions of a sample comprising the modified target molecule.

Abstract translation: 本发明涉及经修饰以便于在纳米孔装置中检测的靶分子。 本发明进一步涉及使用纳米孔装置检测这种修饰的靶分子的方法。 它还公开了使用这种修饰的靶分子用于跟踪和验证药物，化学或生物产品并测量包含修饰的靶分子的样品的各种条件的方法。

公开(公告)号：WO2016049657A1

公开(公告)日：2016-03-31

申请号：PCT/US2015/052729

申请日：2015-09-28

Applicant: TWO PORE GUYS, INC.

Inventor： MORIN, Trevor, J. ,  HELLER, Daniel, A.

IPC: C12Q1/68

CPC classification number: C12Q1/6825 ,  C12Q1/6816 ,  C12Q2525/107 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: Disclosed herein are methods and compositions for detection of one or more specific sequences of polynucleotides in a solution using a nanopore. In some embodiments, methods and compositions for identifying a polynucleotide in a sample or for target sequence detection of a polynucleotide are disclosed herein.

Abstract translation: 本文公开了用于使用纳米孔在溶液中检测多核苷酸的一个或多个特定序列的方法和组合物。 在一些实施方案中，本文公开了用于鉴定样品中的多核苷酸或多核苷酸的靶序列检测的方法和组合物。

公开(公告)号：WO2016187159A2

公开(公告)日：2016-11-24

申请号：PCT/US2016/032784

申请日：2016-05-16

Applicant: TWO PORE GUYS, INC.

Inventor： MORIN, Trevor, J. ,  HELLER, Daniel, A. ,  DUNBAR, William, B.

IPC: C12Q1/68

CPC classification number: C12Q1/6816 ,  C12Q2563/155 ,  C12Q2565/531 ,  C12Q2565/631

Abstract: Provided are methods and compositions for detecting a target analyte suspected to be present in a sample with background molecules using a nanopore device. A plurality of probes for polymer scaffold identification or for target analyte binding and detection are provided.

Abstract translation: 提供了使用纳米孔装置用背景分子检测疑似存在于样品中的目标分析物的方法和组合物。 提供了用于聚合物支架识别或用于靶分析物结合和检测的多个探针。

公开(公告)号：WO2016126746A1

公开(公告)日：2016-08-11

申请号：PCT/US2016/016233

申请日：2016-02-02

Applicant: TWO PORE GUYS, INC.

Inventor： MORIN, Trevor, J. ,  SHROPSHIRE, Tyler ,  DUNBAR, William, B. ,  HELLER, Daniel, A. ,  WANG, Hongyun

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q2531/113 ,  C12Q2563/131 ,  C12Q2565/631

Abstract: Disclosed herein are methods and compositions for detection of target polynucleotides in a mixed sample by amplification of the target polynucleotide and detection in a nanopore device.

Abstract translation: 本文公开了通过扩增靶多核苷酸并在纳米孔装置中检测来检测混合样品中靶多核苷酸的方法和组合物。

公开(公告)号：WO2017173392A8

公开(公告)日：2017-10-05

申请号：PCT/US2017/025585

申请日：2017-03-31

Applicant: TWO PORE GUYS, INC.

Inventor： MORIN, Trevor, J. ,  DUNBAR, William, B. ,  HELLER, Daniel, A. ,  SHROPSHIRE, Tyler

IPC: G01N33/487 ,  G01N33/483 ,  G01N27/66 ,  G01N27/447 ,  C12Q1/68

Abstract: Disclosed herein are methods and compositions for detecting a target DNA sequence from a sample that does not require sample purification or amplification. The method uses fragmentation, sequence-specific binding or ligation of probes, and payload molecules for selective detection of the target-sequence using a nanopore sensor.

公开(公告)号：WO2016145415A1

公开(公告)日：2016-09-15

申请号：PCT/US2016/022210

申请日：2016-03-11

Applicant: TWO PORE GUYS, INC.

Inventor： MORIN, Trevor, J.

IPC: G01N33/00 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53

CPC classification number: G01N33/54366 ,  C12Q1/6816 ,  G01N33/48721 ,  G01N33/54306 ,  C12Q2563/119 ,  C12Q2563/155 ,  C12Q2563/167 ,  C12Q2565/631

Abstract: Disclosed herein are methods and compositions for detection of target small molecules in a mixed sample by performing a competition assay between the target and a surrogate and subsequently detecting the complex types in a nanopore device. Disclosed herein are competition assays for detection of small molecules using a nanopore. Target molecules of a sufficient size (> 20 kDa) when passed through a solid-state nanopore cause a change in the current impedance, translocation time, or other measurable parameter. In the event the target molecule is not sufficiently big, and thus does not cause a noticeable change, an additional molecule/reagent can be used to aid in detection. This detection reagent would bind to the small molecule or to the "capture ligand-molecule complex" (e.g. peptide detection is aided by a monoclonal antibody (mAb) that recognizes a peptide/aptamer complex).

Abstract translation: 本文公开了通过在靶和替代物之间进行竞争测定并随后检测纳米孔装置中的复合物类型来检测混合样品中目标小分子的方法和组合物。 本文公开了使用纳米孔检测小分子的竞争分析。 当通过固态纳米孔时，具有足够大小（> 20kDa）的靶分子导致电流阻抗，易位时间或其他可测量参数的变化。 在目标分子不足够大的情况下，不会引起显着变化，可以使用另外的分子/试剂来辅助检测。 该检测试剂将结合小分子或“捕获配体 - 分子复合物”（例如，肽检测由识别肽/适体复合物的单克隆抗体（mAb）辅助）。