公开(公告)号：WO2013068741A1

公开(公告)日：2013-05-16

申请号：PCT/GB2012/052768

申请日：2012-11-07

Applicant: UCL BUSINESS PLC

Inventor： BRACEWELL, Daniel Gilbert ,  STEVENS, Robert ,  HARDICK, Oliver

IPC: B01J20/285 ,  B01J20/28 ,  B01J20/30 ,  B01D15/36 ,  C07K1/18 ,  C07K1/22 ,  B01D15/18 ,  D01D5/00

CPC classification number: C07K1/16 ,  B01D15/1821 ,  B01D15/361 ,  B01D15/3804 ,  B01J20/24 ,  B01J20/28007 ,  B01J20/28038 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/285 ,  B01J20/3007 ,  B82Y30/00 ,  C07K1/18 ,  C07K1/22

Abstract: The present invention provides a chromatography medium comprising one or more electrospun polymer nanofibres which form a stationary phase comprising a plurality of pores through which a mobile phase can permeate and use of the same in chromatography, such as the isolation of recombinant proteins, monoclonal antibodies, viral vaccines and plasmid DNA. The invention further provides for the use of the chromatographic medium in a simulated moving bed system.

Abstract translation: 本发明提供了一种色谱介质，其包含一个或多个静电聚合物纳米纤维，其形成固定相，其包含多个孔，流动相可通过该孔渗透并在层析中使用它们，例如分离重组蛋白，单克隆抗体， 病毒疫苗和质粒DNA。 本发明还提供了在模拟移动床系统中使用色谱介质。

公开(公告)号：WO2015052465A1

公开(公告)日：2015-04-16

申请号：PCT/GB2014/000401

申请日：2014-10-09

Applicant: UCL BUSINESS PLC

Inventor： HARDICK, Oliver ,  BRACEWELL, Daniel Gilbert ,  DODS, Stewart

IPC: B01J20/24 ,  B01D15/36 ,  B01D15/38 ,  B01J20/28 ,  B01J20/285 ,  B01J20/30 ,  C07K1/18 ,  C07K1/22

CPC classification number: B01J20/265 ,  B01D15/327 ,  B01D15/361 ,  B01D15/362 ,  B01D15/363 ,  B01D15/38 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3819 ,  B01J20/24 ,  B01J20/28007 ,  B01J20/28033 ,  B01J20/28038 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/285 ,  B01J20/287 ,  B01J20/288 ,  B01J20/3007 ,  B01J20/3085 ,  B01J39/05 ,  B01J39/19 ,  B01J39/26 ,  B01J41/07 ,  B01J41/13 ,  B01J41/20 ,  B01J2220/54 ,  B29C65/02 ,  B29C66/45 ,  B29C66/712 ,  B29C66/7294 ,  B29K2601/12 ,  B29K2627/18 ,  B29K2713/00 ,  B29L2009/00 ,  B32B5/022 ,  B32B27/08 ,  B32B27/12 ,  B32B27/322 ,  B32B37/06 ,  B32B37/10 ,  B32B37/182 ,  B32B2262/04 ,  B32B2317/18 ,  B32B2327/18 ,  C07K1/16 ,  C07K1/165 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22

Abstract: The present invention provides a process for preparing a functionalised polymeric chromatography medium, which process comprises (I) providing two or more non- woven sheets stacked one on top of the other, each said sheet comprising one or more polymer nanofibres, (II) simultaneously heating and pressing the stack of sheets to fuse points of contact between the nanofibres of adjacent sheets, and (III) contacting the pressed and heated product with a reagent which functionalises the product of step (II) as a chromatography medium.

Abstract translation: 本发明提供了制备官能化聚合物色谱介质的方法，该方法包括（I）提供两个或更多个层叠在另一个顶部上的非织造片材，每个所述片材同时包含一种或多种聚合物纳米纤维（II） 加热和压制叠片以熔化相邻片材的纳米纤维之间的接触点，和（III）使被加压和加热的产物与将作为色谱介质的步骤（II）的产物起作用的试剂接触。