公开(公告)号：WO2010039921A3

公开(公告)日：2010-07-29

申请号：PCT/US2009059174

申请日：2009-10-01

Applicant: UNIV EAST CAROLINA ,  HU XIN-HUA ,  CHEN CHENG

Inventor： HU XIN-HUA ,  CHEN CHENG

IPC: G01N21/17 ,  G01B11/02 ,  G01N21/41 ,  G01N21/47 ,  G01N33/483

CPC classification number: G01N21/4738 ,  G01N21/4785 ,  G01N2021/4709 ,  G01N2021/4735 ,  G01N2201/0635

Abstract: Methods and systems for optically characterizing a turbid sample are provided. A structured light beam is impinged on the sample. The sample includes an embedded region. A reflected light image of the structured light beam is detected from the sample. A measured reflectance image of the structured light beam for the sample is determined based on the reflected light image and a reflectance standard. The following parameters are determined: absorption coefficients ÿa, scattering coefficient ÿs and anisotropy factor g of the sample from the reflectance image. A size parameter of the embedded region is estimated based on the absorption coefficients ÿa, scattering coefficient ÿs and/or anisotropy factor g of the sample from the measured reflectance image.

Abstract translation: 提供了用于光学表征浑浊样品的方法和系统。 结构化的光束照射在样品上。 该示例包含一个嵌入区域。 从样本中检测结构化光束的反射光图像。 基于反射光图像和反射率标准确定样本的结构化光束的测量反射图像。 确定以下参数：来自反射图像的样品的吸收系数ÿa，散射系数ÿs和各向异性因子g。 嵌入区域的尺寸参数根据来自测量的反射图像的样本的吸收系数ÿa，散射系数ÿs和/或各向异性因子g来估计。

公开(公告)号：WO2012096878A3

公开(公告)日：2012-09-07

申请号：PCT/US2012020626

申请日：2012-01-09

Applicant: UNIV EAST CAROLINA ,  FERGUSON THOMAS BRUCE JR ,  HU XIN-HUA ,  CHEN CHENG

Inventor： FERGUSON THOMAS BRUCE JR ,  HU XIN-HUA ,  CHEN CHENG

IPC: A61B5/00 ,  A61B5/02 ,  A61B5/026 ,  G02B27/48

CPC classification number: A61B5/0261 ,  A61B5/0059 ,  A61B5/02007 ,  G02B27/48 ,  G06T7/0012

Abstract: A non- invasive method for measuring blood flow in principal vessels of a heart of a subject is provided. The method includes illuminating a region of interest in the heart with a coherent light source, wherein the coherent light source has a wavelength of from 600 nm to 1100 nm; sequentially acquiring at least two speckle images of the region of interest in the heart during a fixed time period, wherein sequentially acquiring the at least two speckle images comprises acquiring the at least two speckle images in synchronization with motion of the heart of the subject; and electronically processing the at least two acquired speckle images based on the temporal variation of the pixel intensities in the at least two acquired speckle images to generate a laser speckle contrast imaging (LSCI) image and determine spatial distribution of blood flow rate in the principal vessels and quantify perfusion distribution in tissue in the region of interest in the heart from the LSCI image.

Abstract translation: 提供了一种测量受试者心脏主血管血流量的非侵入性方法。 该方法包括用相干光源照射心脏中的感兴趣区域，其中相干光源具有600nm至1100nm的波长; 在固定时间段内顺序获取心脏中​​的感兴趣区域的至少两个斑点图像，其中顺序获取所述至少两个散斑图像包括与所述对象的心脏的运动同步地获取所述至少两个斑点图像; 并且基于所述至少两个获取的斑点图像中的像素强度的时间变化来电子处理所述至少两个获取的散斑图像，以产生激光散斑对比成像（LSCI）图像，并确定主血管中的血流速率的空间分布 并从LSCI图像中量化心脏中感兴趣区域的组织中的灌注分布。

公开(公告)号：WO2009151610A3

公开(公告)日：2010-03-11

申请号：PCT/US2009003508

申请日：2009-06-11

Applicant: UNIV EAST CAROLINA ,  HU XIN-HUA ,  JACOBS KENNETH M ,  LU JUN O

Inventor： HU XIN-HUA ,  JACOBS KENNETH M ,  LU JUN O

IPC: G01N15/14 ,  G01N33/487

CPC classification number: G01N15/1475 ,  G01N15/0211 ,  G01N15/147 ,  G01N2015/0222 ,  G01N2015/025 ,  G01N2015/1497

Abstract: A flow cytometer assembly includes a fluid controller configured to form a hydrodynamically focused flow stream including an outer sheath fluid and an inner core fluid. A coherent light source is configured to illuminate a particle in the inner core fluid. A detector is configured to detect a spatially coherent distribution of elastically scattered light from the particle excited by the coherent light source. An analyzing module configured to extract a three-dimensional morphology parameter of the particle from a spatially coherent distribution of the elastically scattered light.

Abstract translation: 流式细胞仪组件包括流体控制器，该流体控制器被配置为形成包括外护套流体和内芯流体的流体动力学聚焦的流动流。 相干光源被配置为照亮内芯流体中的颗粒。 检测器被配置为检测来自由相干光源激发的粒子的弹性散射光的空间相干分布。 分析模块，被配置为从所述弹性散射光的空间相干分布中提取所述粒子的三维形态参数。