公开(公告)号：WO0212284A3

公开(公告)日：2003-08-21

申请号：PCT/US0124747

申请日：2001-08-06

Applicant: UNIV LOMA LINDA MED ,  US GOV HEALTH & HUMAN SERV

Inventor： KIRSCH WOLFF M ,  LENNART ANTON ,  KELLN WAYNE J ,  KANG DAE-KYUNG ,  LEVINE RODNEY L ,  ROUALT TRACEY A

IPC: C12N15/09 ,  C07K14/00 ,  C07K14/47 ,  C07K16/18 ,  C12P21/08 ,  C12Q1/68

CPC classification number: G01N33/6896 ,  C07K14/4702 ,  C07K16/18 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N2800/04 ,  G01N2800/2821

Abstract: The present invention relates to the discovery of markers for neurodegenerative disease. More particularly, it was discovered that forms of IRP-2 protein that are unable to undergo oxidation at critical cysteine residues are diagnostic for neurodegenerative disease including, but not limited to Alzheimer's Disease (AD). Embodiments include nucleic acids that encode mutant IRP-2 proteins and fragments thereof, mutant IRP-2 proteins and fragments thereof, antibodies directed to epitopes present on mutant IRP-2 proteins and fragments thereof, methods of making these nucleic acids and polypeptides, as well as, approaches to diagnose neurodegenerative disease in animals, such as humans at risk of contracting AD or mild cognitive impairment syndrome (MCI). The level or distribution of iron in a human brain, as determined by magnetic resonance imaging (MRI), can be used to diagnose AD and/or MCI.

Abstract translation: 本发明涉及神经变性疾病标记物的发现。 更具体地，已经发现不能在关键半胱氨酸残基处经历氧化的IRP-2蛋白的形式是对神经变性疾病的诊断，包括但不限于阿尔茨海默病（AD）。 实施方案包括编码突变体IRP-2蛋白及其片段，突变体IRP-2蛋白及其片段的核酸，针对存在于突变体IRP-2蛋白及其片段上的表位的抗体，以及制备这些核酸和多肽的方法 作为诊断动物中的神经变性疾病的方法，例如患有AD或轻度认知障碍综合征（MCI）的风险的人。 通过磁共振成像（MRI）测定的人脑中铁的水平或分布可用于诊断AD和/或MCI。