公开(公告)号：WO2016180421A1

公开(公告)日：2016-11-17

申请号：PCT/DK2016/050122

申请日：2016-05-11

申请人： UNIVERSITY OF COPENHAGEN

发明人： JAEL RUBNER FRIDRIKSDOTTIR, Agla ,  KIM, Jiyoung ,  VILLADSEN, René ,  HOPKINSSON, Branden ,  CHRISTINE KLITGAARD, Marie ,  WILLIAM PETERSEN, Ole ,  RØNNOV-JESSEN PETERSEN, Lone

IPC分类号： C12N5/09 ,  C12N5/00 ,  C12N5/071

CPC分类号： C12N5/0631 ,  C12N2500/25 ,  C12N2500/46 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2503/02 ,  C12N2510/00 ,  C12N2510/04 ,  G01N33/5091

摘要： The present invention describes methods for long-term culturing of ER pos cells, cell lines, and/or cell strains with extended lifespan and/or cell strain as well as culture medium compositions. The invention further describes methods for isolating cells which may be used as starting point for long-term culturing of ER pos cells, cell lines, and/or cell strains with extended lifespan and/or cell strain. The invention further discloses various methods for generating ER pos tumorigenic cells, cell lines, and/or cell strains with extended lifespan and/or cell strain as well as various assays for their use.

摘要翻译： 本发明描述了长期培养具有延长的寿命和/或细胞应变以及培养基组合物的ERpos细胞，细胞系和/或细胞株的方法。 本发明还描述了分离细胞的方法，其可以用作长期培养具有延长的寿命和/或细胞应变的ER多肽细胞，细胞系和/或细胞株的起点。 本发明进一步公开了用于产生具有延长的寿命和/或细胞应变以及其使用的各种测定法的ERpos致瘤细胞，细胞系和/或细胞株的各种方法。