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    METHODS AND COMPOSITIONS FOR PROMOTING OPC DIFFERENTIATION AND REMYELINATION USING RECEPTOR ASSOCIATED PROTEIN (RAP)
    3.
    发明申请
    METHODS AND COMPOSITIONS FOR PROMOTING OPC DIFFERENTIATION AND REMYELINATION USING RECEPTOR ASSOCIATED PROTEIN (RAP) 审中-公开
    使用受体相关蛋白（RAP）促进OPC分化和再生的方法和组合物

    公开(公告)号：WO2017165462A1

    公开(公告)日：2017-09-28

    申请号：PCT/US2017/023481

    申请日：2017-03-21

    申请人： NOVORON BIOSCIENCE, INC.

    发明人： STILES, Travis

    IPC分类号： C12N5/0793 ,  C12N15/113 ,  C12N15/86 ,  A61K38/17 ,  C07K16/28 ,  C07K14/705

    CPC分类号： A61K38/177 ,  A61K9/0019 ,  A61P25/00 ,  C07K14/47 ,  C07K14/70596 ,  C12N5/0622 ,  C12N15/86 ,  C12N2501/395 ,  C12N2501/998

    摘要： The present disclosure relates to methods and compositions using RAP, a derivative of RAP, a variant of RAP, or a fragment of RAP to inhibit LRP1, a myelin debris receptor. The methods and compositions involve increasing, promoting, restoring, and/or enhancing differentiation of oligodendrocyte progenitor cells, myelin protein expression, mature oligodendrocyte marker expression, and/or myelination. The methods and compositions disclosed herein inhibit or block pathological activation of RhoA in OPCs. The methods and compositions also involve alleviating one or more symptoms of MS and treating MS, including slowing or stopping MS progression.

    摘要翻译： 本公开涉及使用RAP，RAP的衍生物，RAP的变体或RAP的片段来抑制髓磷脂碎片受体LRP1的方法和组合物。 所述方法和组合物涉及增加，促进，恢复和/或增强少突神经胶质祖细胞的分化，髓磷脂蛋白质表达，成熟少突胶质细胞标志物表达和/或髓鞘形成。 本文公开的方法和组合物在OPC中抑制或阻断RhoA的病理活化。 所述方法和组合物还涉及减轻MS的一种或多种症状并治疗MS，包括减缓或停止MS进展。

    METHODS OF DIFFERENTIATING RETINAL CELLS
    4.
    发明申请
    METHODS OF DIFFERENTIATING RETINAL CELLS 审中-公开
    分化视网膜细胞的方法

    公开(公告)号：WO2017091844A1

    公开(公告)日：2017-06-08

    申请号：PCT/AU2016/000390

    申请日：2016-12-05

    申请人： BOND UNIVERSITY LTD

    发明人： IOANNiS, Jason Limnios

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0621 ,  C12N2500/98 ,  C12N2501/385 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2533/52 ,  C12N2533/90

    摘要： The invention provides a differentiation protocol to generate retinal pigmented epithelial cells (RPECs) for the in vitro differentiation of mammalian pluripotent stem cells to mature, functional retinal pigment epithelial cells (RPEC). A culture system is described that uses defined medium comprising xeno-free optimal combinations of small molecule agonists and antagonists where: (a) pluripotent stem cells are cultured in the absence of feeder cells, serum, or conditioned medium; (b) defined culture conditions is used to obtain an eye-field cell (EFC) population; (c) whereby EFC are subsequently differentiated to RPEC when exposed to culture medium containing small molecule agonists thus producing highly homogenous RPEC; and (d) cultured under optimal conditions until maturation, through stimulation via activin-like signalling through addition of either cyclic AMP or cyclic AMP inducers to produce complete monolayer formation within 21 +7 days. An alternate differentiation procedure also takes EFC intermediates prepared above into photoreceptors.

    摘要翻译： 本发明提供了产生用于将哺乳动物多能干细胞体外分化为成熟的功能性视网膜色素上皮细胞（RPEC）的视网膜色素上皮细胞（RPEC）的分化方案。 描述了使用包含小分子激动剂和拮抗剂的无异源最佳组合的确定培养基的培养系统，其中：（a）在缺乏饲养细胞，血清或条件培养基的情况下培养多能干细胞; （b）确定的培养条件用于获得眼部细胞（EFC）群体; （c）当EFC暴露于含有小分子激动剂的培养基中时，随后分化成RPEC，从而产生高度均一的RPEC; 和（d）在最佳条件下培养直至成熟，通过加入环AMP或环AMP诱导剂经由激活素样信号传导刺激以在21 + 7天内产生完全的单层形成。 另一种分化程序也将EFC中间体制备成光感受器。

    USE OF DERMATOPONTIN FOR MAINTAINING HEMATOPOIETIC STEM AND/OR PROGENITOR CELLS IN CULTURE
    6.
    发明申请
    USE OF DERMATOPONTIN FOR MAINTAINING HEMATOPOIETIC STEM AND/OR PROGENITOR CELLS IN CULTURE 审中-公开
    用于维持文化中HEMATOPOIETIC STEM和/或PROGENITOR细胞的DERMATOPONTIN的使用

    公开(公告)号：WO2017021543A1

    公开(公告)日：2017-02-09

    申请号：PCT/EP2016/068790

    申请日：2016-08-05

    申请人： HELMHOLTZ ZENTRUM MÜNCHEN - DEUTSCHES FORSCHUNGSZENTRUM FÜR GESUNDHEIT UND UMWELT (GMBH) ,  EIDGENÖSSISCHE TECHNISCHE HOCHSCHULE ZÜRICH

    发明人： KOKKALIARIS, Konstantinos ,  SCHROEDER, Timm

    IPC分类号： C12N5/0789 ,  A61K35/12

    CPC分类号： C12N5/0647 ,  A61K35/12 ,  C12N2500/90 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/998 ,  C12N2502/13 ,  C12N2533/54 ,  C12N2533/90

    摘要： The present invention relates to the use of dermatopontin (DPT) or a functional fragment thereof for the maintenance of hematopoietic stem and/or progenitor cells in culture. The present invention further relates to a method for maintaining hematopoietic stem and/or progenitor cells in culture, the method comprising culturing the hematopoietic stem and/or progenitor cells in the presence of dermatopontin (DPT) or a functional fragment thereof. Furthermore, the present invention relates to a cell culture medium for the maintenance of hematopoietic stem and/or progenitor cells, wherein the cell culture medium comprises a medium and dermatopontin (DPT) or a functional fragment thereof and further optionally comprises serum/serum replacement, (a) reducing agent(s), and/or (an) antibiotic(s) as well as a kit comprising dermatopontin (DPT) or a functional fragment thereof and at least one of: (a) (a) cell culture medium; (b) one or more cytokines; (c) serum/serum replacement; (d) (a) reducing agent(s), and/or (e) (an) antibiotic(s).

    摘要翻译： 本发明涉及皮肤素（DPT）或其功能片段用于维持培养中造血干细胞和/或祖细胞的用途。 本发明还涉及用于在培养物中维持造血干细胞和/或祖细胞的方法，所述方法包括在皮肤素（DPT）或其功能片段的存在下培养造血干细胞和/或祖细胞。 此外，本发明涉及用于维持造血干细胞和/或祖细胞的细胞培养基，其中细胞培养基包含培养基和皮肤粘蛋白（DPT）或其功能片段，并且还任选地包含血清/血清替代物， （a）还原剂和/或（一种）抗生素以及包含皮肤素（DPT）或其功能性片段的试剂盒和至少一种：（a）（a）细胞培养基; （b）一种或多种细胞因子; （c）血清/血清替代物; （d）（a）还原剂和/或（e）（a）抗生素。

    UNIVERSAL DONOR STEM CELLS AND RELATED METHODS
    7.
    发明申请
    UNIVERSAL DONOR STEM CELLS AND RELATED METHODS 审中-公开
    通用干细胞和相关方法

    公开(公告)号：WO2016183041A3

    公开(公告)日：2017-01-05

    申请号：PCT/US2016031551

    申请日：2016-05-09

    申请人： HARVARD COLLEGE

    发明人： MEISSNER TORSTEN B ,  FERREIRA LEONARDO M R ,  STROMINGER JACK L ,  COWAN CHAD A

    IPC分类号： A61K48/00 ,  C07K14/74 ,  C12N5/0735

    CPC分类号： A61K39/001 ,  C12N5/0606 ,  C12N5/0696 ,  C12N2501/50 ,  C12N2501/599 ,  C12N2501/998 ,  C12N2510/00

    摘要： Disclosed herein are universal donor stem cells and related methods of their use and production. The universal donor stem cells disclosed herein are useful for overcoming the immune rejection in cell-based transplantation therapies. In certain embodiments, the universal donor stem cells disclosed herein do not express one or more MHC-I and MHC-II human leukocyte antigens. Similarly, in certain embodiments, the universal donor stem cells disclosed herein do not express one or more human leukocyte antigens (e.g., HLA-A, HLA-B and/or HLA-C) corresponding to MHC-I and MHC-II human leukocyte antigens, thereby rendering such cells hypoimmunogenic.

    摘要翻译： 本文公开了通用供体干细胞及其使用和生产的相关方法。 本文公开的通用供体干细胞可用于克服基于细胞的移植治疗中的免疫排斥。 在某些实施方案中，本文公开的通用供体干细胞不表达一种或多种MHC-1和MHC-II人白细胞抗原。 类似地，在某些实施方案中，本文公开的通用供体干细胞不表达对应于MHC-1和MHC-II人白细胞的一种或多种人白细胞抗原（例如，HLA-A，HLA-B和/或HLA-C） 抗原，从而使这种细胞免疫原性降低。