公开(公告)号：WO2021260011A1

公开(公告)日：2021-12-30

申请号：PCT/EP2021/067160

申请日：2021-06-23

Applicant: UNIVERSITY OF LIMERICK

Inventor： FAIR, Sean ,  NEWPORT, David ,  O'SULLIVAN, Leonard ,  WHITE, Eoin ,  O'SULLIVAN, Kevin

IPC: C12M3/06 ,  C12M1/00 ,  C12N5/00 ,  C12N5/076 ,  C12M1/34 ,  C12M1/36 ,  C12M1/26 ,  C12M21/06 ,  C12M23/16 ,  C12M33/04 ,  C12M41/00 ,  C12M47/04 ,  C12N5/0612

Abstract: According to the present invention there is provided a sample separating device for separating motile organisms from other organisms, the device comprising: one or more primary fluid paths extending between a fluid inlet and a fluid terminus such that a fluid flow may be established between the fluid inlet and the fluid terminus; a sample introduction zone in the primary fluid path for introducing a sample to the primary fluid path; a sample collection zone for collecting a collected sample therein, the sample collection zone being located at a location upstream of the sample introduction zone in the primary fluid path; a sample collection zone outlet for facilitating removal of the collected sample from the sample collection zone; the sample separating device being arranged to allow communication with a sample moving system for moving the collected sample through the sample collection zone, thereby to move at least some of the collected sample from the sample collection zone to the sample collection zone outlet.

公开(公告)号：WO2021260012A1

公开(公告)日：2021-12-30

申请号：PCT/EP2021/067161

申请日：2021-06-23

Applicant: UNIVERSITY OF LIMERICK

Inventor： FAIR, Sean ,  NEWPORT, David ,  ROMERO AGUIRREGOMEZCORTA, Jon ,  O'SULLIVAN, Leonard ,  WHITE, Eoin ,  O'SULLIVAN, Kevin

IPC: C12M3/06 ,  C12M1/00 ,  C12N5/00 ,  C12N5/076 ,  C12M1/34 ,  C12M1/36 ,  C12M21/06 ,  C12M23/16 ,  C12M33/04 ,  C12M41/00 ,  C12M47/04 ,  C12N5/0612

Abstract: A method for separating motile organisms from other organisms. The method comprises controlling a fluid delivery unit to provide a fluid flow to a sample separating device (302). The fluid flow has a sample introduction flow velocity set so that a sample may be introduced into a sample introduction zone of the device. The sample introduction flow velocity is sufficiently high such that an organism in the sample is unable to exit the sample introduction zone. The method comprises controlling the fluid delivery unit to reduce the fluid flow velocity from the sample introduction flow velocity to an operational flow velocity lower than the sample introduction flow velocity (303). The operational flow velocity is selected such that motile organisms in the sample are able to swim against the fluid flow and enter a sample collection zone of the device.