公开(公告)号：WO2022178488A2

公开(公告)日：2022-08-25

申请号：PCT/US2022/070542

申请日：2022-02-05

Applicant: CYTEK BIOSCIENCES, INC.

Inventor： KRUEGER, Glen ,  VRANE, David

IPC: G01N15/14 ,  G01N21/49 ,  G01N21/63 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1844 ,  B01L2300/1894 ,  B01L3/0241 ,  B01L3/502715 ,  B01L3/502761 ,  C12M47/04 ,  G01N15/1436 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1409 ,  G01N2015/149

Abstract: A flow cytometry or cell sorting system is disclosed. Under pressure, a fluidics system causes sheath and sample biological fluids to flow. The fluidics system can include a degasser. A flow cell communicates with the fluidics system to receive the sheath fluid, wherein a sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. A deflection chamber under the flow cell receives the drops of sample biological fluid and sheath fluid out of the flow cell to selectively deflect one or more of the drops along one or more deflection paths; and a droplet deposition unit to receive selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.

公开(公告)号：WO2020154484A1

公开(公告)日：2020-07-30

申请号：PCT/US2020/014758

申请日：2020-01-23

Applicant: BECTON, DICKINSON AND COMPANY

Inventor： XU, Qihua ,  WEIDEMAIER, Kristin ,  SALOMON, John, E. ,  LASTOVICH, Alexander, G. ,  FALLOWS, Eric, A. ,  CONNELL, Sean ,  HERR, Joshua ,  WOLFGANG, Meghan ,  BRASCH, Michael, A. ,  MOORE, Richard, L. ,  SEBBA, David, S. ,  CLAVIJO, Cristian ,  NG, Shirley ,  ABBOT, Richard ,  PAPP, Alexander, Adam ,  FU, Henry, Li-wei ,  AUSTIN, Caitlin, Marie ,  DOWLING, Sean, Patrick ,  JOYCE, Owen, Lewis ,  KIPLINGER, Michael, L. ,  CARPENTER, William, Kevin

IPC: B01L3/00 ,  G01N1/40 ,  A61M5/165 ,  B01D61/18 ,  C12M1/00 ,  A61M2005/1657 ,  B01D2311/25 ,  B01D2315/10 ,  B01D61/142 ,  B01D63/087 ,  B01D67/0032 ,  B01L2200/0652 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0478 ,  B01L2400/049 ,  B01L3/502753 ,  B01L3/502761 ,  C12M33/14 ,  C12M47/04 ,  G01N1/4005 ,  G01N1/4077 ,  G01N2001/4088

Abstract: Methods and apparatus provide filtration for concentrating analytes, such as bacteria or exosomes, of a biological sample, such as blood or urine. The technology may employ membrane devices that implement one or more tangential flow filtration processes such as in stages. An example membrane device may typically include a membrane having sides and ends. The membrane may selectively permit constituent(s) of the sample to pass through while retaining other constituents at one side. An input chamber of the device may include an inlet near one end and an outlet near the other end, and that may permit a tangential flow of the sample along the first side surface, and a trans-membrane passing of constituent(s). An output chamber of the device may be configured at the second side surface to receive the passing constituents. Such devices may be provided in a kit to facilitate targeting of a desired biological analyte concentration.

公开(公告)号：WO2021260012A1

公开(公告)日：2021-12-30

申请号：PCT/EP2021/067161

申请日：2021-06-23

Applicant: UNIVERSITY OF LIMERICK

Inventor： FAIR, Sean ,  NEWPORT, David ,  ROMERO AGUIRREGOMEZCORTA, Jon ,  O'SULLIVAN, Leonard ,  WHITE, Eoin ,  O'SULLIVAN, Kevin

IPC: C12M3/06 ,  C12M1/00 ,  C12N5/00 ,  C12N5/076 ,  C12M1/34 ,  C12M1/36 ,  C12M21/06 ,  C12M23/16 ,  C12M33/04 ,  C12M41/00 ,  C12M47/04 ,  C12N5/0612

Abstract: A method for separating motile organisms from other organisms. The method comprises controlling a fluid delivery unit to provide a fluid flow to a sample separating device (302). The fluid flow has a sample introduction flow velocity set so that a sample may be introduced into a sample introduction zone of the device. The sample introduction flow velocity is sufficiently high such that an organism in the sample is unable to exit the sample introduction zone. The method comprises controlling the fluid delivery unit to reduce the fluid flow velocity from the sample introduction flow velocity to an operational flow velocity lower than the sample introduction flow velocity (303). The operational flow velocity is selected such that motile organisms in the sample are able to swim against the fluid flow and enter a sample collection zone of the device.

公开(公告)号：WO2021260011A1

公开(公告)日：2021-12-30

申请号：PCT/EP2021/067160

申请日：2021-06-23

Applicant: UNIVERSITY OF LIMERICK

Inventor： FAIR, Sean ,  NEWPORT, David ,  O'SULLIVAN, Leonard ,  WHITE, Eoin ,  O'SULLIVAN, Kevin

IPC: C12M3/06 ,  C12M1/00 ,  C12N5/00 ,  C12N5/076 ,  C12M1/34 ,  C12M1/36 ,  C12M1/26 ,  C12M21/06 ,  C12M23/16 ,  C12M33/04 ,  C12M41/00 ,  C12M47/04 ,  C12N5/0612

Abstract: According to the present invention there is provided a sample separating device for separating motile organisms from other organisms, the device comprising: one or more primary fluid paths extending between a fluid inlet and a fluid terminus such that a fluid flow may be established between the fluid inlet and the fluid terminus; a sample introduction zone in the primary fluid path for introducing a sample to the primary fluid path; a sample collection zone for collecting a collected sample therein, the sample collection zone being located at a location upstream of the sample introduction zone in the primary fluid path; a sample collection zone outlet for facilitating removal of the collected sample from the sample collection zone; the sample separating device being arranged to allow communication with a sample moving system for moving the collected sample through the sample collection zone, thereby to move at least some of the collected sample from the sample collection zone to the sample collection zone outlet.

公开(公告)号：WO2021127175A1

公开(公告)日：2021-06-24

申请号：PCT/US2020/065607

申请日：2020-12-17

Applicant: THE SECANT GROUP, LLC

Inventor： GINN, Brian ,  GABRIELE, Peter, D. ,  HARRIS, Jeremy, J. ,  WEBER, Amanda, K.

IPC: C12M3/00 ,  C12M1/12 ,  C12M23/42 ,  C12M23/44 ,  C12M23/58 ,  C12M25/02 ,  C12M25/16 ,  C12M29/04 ,  C12M47/04 ,  C12M47/10

Abstract: A modular flow-through cartridge bioreactor system includes a plurality of modular flow-through cartridges. Each modular flow-through cartridge includes a cartridge housing with ports for through flow of a biological media and predetermined contents preloaded in the cartridge housing permitting the cartridge to perform at least one predetermined function of the bioreactor process upon through-flow of the biological media. The modular flow-through cartridge bioreactor system also includes at least one interlock connector fluidly connecting the plurality of modular flow-through cartridges by the ports. A modular flow through cartridge includes rows of porous textiles preloaded in the cartridge housing. A process includes selecting a plurality of modular flow-through cartridges to perform, in combination, a bioreactor process. The process also includes fluidly connecting the modular flow-through cartridges in a fluid sequence to form the modular flow-through cartridge bioreactor system. Flowing the biological media through the fluid sequence performs the bioreactor process.

公开(公告)号：WO2021124111A1

公开(公告)日：2021-06-24

申请号：PCT/IB2020/061977

申请日：2020-12-15

Applicant: KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： RAKHYMZHANOV, Almas ,  BUTTNER, Ulrich

IPC: B01L3/00 ,  C12N5/071 ,  B01L2200/0652 ,  B01L2200/12 ,  B01L2300/0874 ,  B01L2400/0457 ,  B01L3/502761 ,  C12M47/04 ,  C12N5/0612

Abstract: A sperm-sorting microchannels device (700) includes a collecting chamber (710) configured to receive a sorting medium (712); a loading chamber (720) configured to receive raw sperm cells (752); and plural microchannels (730) extending between the collecting chamber (710) and the loading chamber (720). A bottom (710A) of the collecting chamber (710) and a bottom (720A) of the loading chamber (720) are in a same plane (711).

公开(公告)号：WO2022241138A1

公开(公告)日：2022-11-17

申请号：PCT/US2022/029026

申请日：2022-05-12

Applicant: JANSSEN BIOTECH, INC. ,  IMEC VZW

Inventor： PEUMANS, Peter ,  TULLOCH, Alexander ,  HENRY, Olivier ,  VAN ROY, Willem ,  STAKENBORG, Tim

IPC: C12M1/00 ,  B01L3/00 ,  G01N15/14 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/16 ,  B01L2400/086 ,  B01L2400/088 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  C12M35/04 ,  C12M47/04 ,  G01N15/147 ,  G01N2015/1006 ,  G01N2015/1454 ,  G01N2015/149 ,  G03H1/00

Abstract: A device for sorting biological cells comprises a flow channel configured to pass a flow of liquid carrying the biological ceils. The flow channel comprises at least one zone associated with a cell category. Each zone of the flow channel comprises at least one surface coated with molecules having an affinity to the cell category associated with the zone, the at least one surface of the zone being configured to modify, by the molecules of the surface, a movement of a cell belonging to the associated cell category of the zone as the cell passes the zone. The device comprises a detector configured to detect a cell exhibiting at least one modified movement in one zone of the flow channel, and to transmit a trigger signal based on the detection of the cell. The device further comprises an actuator configured to divert the detected cell based on the trigger signal.

公开(公告)号：WO2022187319A2

公开(公告)日：2022-09-09

申请号：PCT/US2022/018458

申请日：2022-03-02

Applicant: UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION, INC.

Inventor： CENTNER, Connor ,  KOPECHEK, Jonathan ,  BATES, Paula

IPC: B01D15/38 ,  B01J20/24 ,  B01J20/28 ,  B01D21/28 ,  C12M47/04

Abstract: Devices and methods enable in-line separation/filtration of cells or particles during fill- and-finish operations without requiring external processing steps. Cell/particle separation and volume reduction is achieved using cationic microbubbles or microparticles in combination with acoustic radiation force via high pressure ultrasound waves, which deflect the cells or particles toward the wall to concentrate them into one side of the channel. The rate of cell/particle movement is directly dependent on their acoustic contrast, which is primarily a function of size, density, and compressibility. Therefore, cells or particles with different size, density, or compressibility can be separated with this technique.

公开(公告)号：WO2022055859A1

公开(公告)日：2022-03-17

申请号：PCT/US2021/049251

申请日：2021-09-07

Applicant: SUNFLOWER THERAPEUTICS, LLC

Inventor： BANCHIERI, Andrew ,  LIU, Flora ,  GRAHAM, Marc, Miller

IPC: C12M1/26 ,  C12M1/00 ,  B01D2313/06 ,  B01D2313/08 ,  B01D2313/12 ,  B01D2325/02 ,  B01D2325/20 ,  B01D61/14 ,  B01D63/06 ,  B01D63/065 ,  B01D69/02 ,  B01D69/10 ,  B01D71/10 ,  B01D71/16 ,  B01D71/34 ,  B01D71/50 ,  B01D71/68 ,  C12M23/06 ,  C12M29/04 ,  C12M33/14 ,  C12M41/40 ,  C12M47/02 ,  C12M47/04

Abstract: A cell retention device includes a structured support with a plurality of circumferentially distributed ribs to retain the active filtering surface of a flexible, porous membrane filter medium. The filter medium surrounds the support in contact with the peaks of the ribs, thereby forming axial voids between the rib peaks. This arrangement imparts sufficient structural support over small regions of the filter medium to facilitate its use in a circular (or other rounded) configuration while providing sufficient channel volume to support high throughput of fluid sparse of cells.

公开(公告)号：WO2022005737A1

公开(公告)日：2022-01-06

申请号：PCT/US2021/037394

申请日：2021-06-15

Applicant: THE SECANT GROUP, LLC

Inventor： HARRIS, Jeremy J. ,  GABRIELE, Peter D. ,  GINN, Brian

IPC: C12M1/26 ,  C12M1/42 ,  C12M1/00 ,  G01N33/543 ,  C12M33/14 ,  C12M35/00 ,  C12M47/04 ,  C12N5/0636 ,  G01N33/54313 ,  G01N33/545 ,  G01N33/56966

Abstract: A cell selection and sorting process includes attaching cells of a target cell type to a first set of polymeric beads, washing the chamber through a first filter having a first pore size less than the first bead diameter to retain the first set of polymeric beads and greater than a cell diameter to remove unattached cells, releasing the cells of the target cell type from the first set of polymeric beads, and collecting the cells of the target cell type. A cell modification process includes modifying cells of the target cell type in the chamber. A cell modification system includes a cell modification chamber with entry ports and outlet ports, filters with predetermined pore sized selectably located on the outlet ports, and sets of polymeric beads with predetermined diameters being selected such that the sets of polymeric beads are separable by the filters.