公开(公告)号：WO2021154923A2

公开(公告)日：2021-08-05

申请号：PCT/US2021/015393

申请日：2021-01-28

申请人： VOYAGER THERAPEUTICS, INC. ,  MORRISON, Christopher J.

发明人： MATHUR, Krishanu ,  HENDRICKS, Andrade ,  LUTHER, Matthew ,  CARDINAL, Jacob J. ,  HURWIT, Daniel S. ,  KARPES, Lori B.

IPC分类号： C12N15/86 ,  C12N15/864 ,  C12N15/866 ,  C12N7/02 ,  A61K48/00 ,  C12N2710/14143 ,  C12N2710/14144 ,  C12N2750/14143 ,  C12N2750/14151

摘要： The present disclosure describes methods and systems for use in the production of recombinant adeno-associated virus (rAAV) particles comprising a payload (e.g., a polynucleotide encoding aromatic L-amino acid decarboxylase (AADC) or a functional variant thereof). In certain embodiments, the production process uses Sf9 insect cells as viral production cells. In certain embodiments, the production process and system use Baculoviral Expression Vectors (BEVs) and/or Baculoviral Infected Insect Cells (BIICs) in the production of rAAV particles.

公开(公告)号：WO2020150556A1

公开(公告)日：2020-07-23

申请号：PCT/US2020/014000

申请日：2020-01-17

申请人： VOYAGER THERAPEUTICS, INC.

发明人： MARANGA, Luis ,  MORRISON, Christopher J. ,  MATHUR, Krishanu ,  LUTHER, Matthew ,  HURWIT, Daniel S. ,  CARDINAL, Jacob J. ,  KARPES, Lori B. ,  ANSONDARIA, Aditya ,  FORSTER, James ,  DISMUKE, David ,  STEININGER, Robert

IPC分类号： A61P25/14 ,  C12N15/86

摘要： The present disclosure describes methods and systems for use in the production of adeno-associated virus (AAV) particles, comprising recombinant adeno-associated virus (rAAV) particles. In certain embodiments, the production process and system use Sf9 insect cells as viral production cells. In certain embodiments, the production process and system use Baculoviral Expression Vectors (BEVs) and Baculoviral Infected Insect Cells (BIICs) in the production of AAV particles.

公开(公告)号：WO2021030125A1

公开(公告)日：2021-02-18

申请号：PCT/US2020/045093

申请日：2020-08-06

申请人： VOYAGER THERAPEUTICS, INC.

发明人： MATHUR, Krishanu ,  SLADE, Peter ,  NEUMAN, Anastasia ,  LY, Charles

IPC分类号： C12N7/00 ,  C12N15/86 ,  C12N15/861 ,  C07K14/005 ,  A61K48/00

摘要： The present disclosure describes methods and systems for use in the production of adeno-associated virus (AAV) particles, compositions and formulations, including recombinant adeno-associated viruses (rAAV). The present disclosure presents cell culture mediums for use in producing adeno-associated viruses (AAV), such as AAV which comprise a polynucleotide encoding a payload. In certain embodiments, the cell culture medium comprises a hydrolysate mixture, L-glutamine, poloxamer 188 (e.g. 10% pluronic F- 68), a lipid emulsion, and a cholesterol mixture. In certain embodiments, the production process and system use Spodoptera frugiperda insect cells (such as Sf9 or Sf21) as viral production cells (VPCs). In certain embodiments, the production process and system use Baculoviral Expression Vectors (BEVs) and/or Baculoviral Infected Insect Cells (BIICs) in the production of rAAVs.

公开(公告)号：WO2022032153A1

公开(公告)日：2022-02-10

申请号：PCT/US2021/045037

申请日：2021-08-06

申请人： VOYAGER THERAPEUTICS, INC.

发明人： MATHUR, Krishanu ,  SLADE, Peter ,  NEUMAN, Anastasia ,  LY, Charles ,  YI, Dongyang ,  GUILLEMIN, Zeynep

IPC分类号： A61K48/00 ,  C12N7/00 ,  C12N15/861

摘要： The present disclosure describes methods and systems for use in the production of adeno- associated virus (AAV) particles, compositions, and formulations, including recombinant adeno-associated viruses (rAAV). The present disclosure presents cell culture mediums for use in producing adeno-associated viruses (AAV), such as AAV which comprise a polynucleotide encoding a payload. In certain embodiments, the cell culture medium comprises a hydrolysate mixture, L-glutamine, poloxamer 188 (e.g., 10% pluronic F-68), a lipid emulsion, and a cholesterol mixture. In certain embodiments, the production process and system use Spodoptera fmgiperda insect cells (such as Sf9 or Sf21) as viral production cells (VPCs). In certain embodiments, the production process and system use Baculo viral Expression Vectors (BEVs) and/or Baculoviral Infected Insect Cells (BIICs) in the production of rAAVs.