摘要:
A device (10) for characterization of an optical beam of at least one pulse includes a beam splitter (14) for splitting the optical beam (12) into at least a first beam (12a) and a second beam (12b), and a spectral separator (16) for spectrally separating at least one monochromatic wavelength component of the first beam. The device includes a photosensitive detector (20) for detecting the second beam and one of the monochromatic wavelength components (12c) of the first beam. The device further includes a processor (24) for determining a difference between the time of detection of the pulse of the second beam and the time of detection of the corresponding pulse of the monochromatic wavelength component of the first beam.
摘要:
Methods, apparatus, and systems for focusing light and remote imaging through a multi-mode fiber without pre-calibration and without direct localized feedback from the distal end of the fiber. Focus control is performed by optimally shaping the wavefront input to the fiber's proximal end according to feedback information embedded in back- propagating fluorescence from the distal end, via processing that exploits the partial cylindrical symmetry of the fiber, and which optimizes according to a weighting mask. Also included is a method for efficient photo-bleaching of a fluorescently-tagged sample, which optimizes the speed and efficiency of the photo-bleaching while minimizing or avoiding damage to non-tagged areas of the sample.