Abstract:
La présente invention se rapporte à un procédé de préparation d'un substitut de peau, à un procédé de préparation d'un substitut de derme, à un substitut de peau, à un substitut de derme et à un kit pour la mise en œuvre du procédé. La présente invention se rapporte également à un greffon constitué d'un substitut de peau et son utilisation comme moyen de traitement d'un désordre cutané et/ou d'une perte de substance cutanée. La présente invention trouve une application notamment dans les domaines pharmacologique, médical, clinique.
Abstract:
The present invention is directed to a method of preparing an artificial tooth primordium in vitro, comprising the steps: a) providing isolated mesenchymal dental pulp cells; and b) culturing the mesenchymal dental pulp cells under non-adherent conditions to form a cell aggregate representing an artificial tooth primordium; as well as to an artificial tooth primordium derived therefrom.
Abstract:
본 발명은 섬유아세포, 케라티노사이트 및 멜라노사이트 중 하나 이상의 세포를 포함하는 하나 이상의 층을 포함하는 피부 미백 물질 스크리닝 시스템 및 상기 시스템을 이용하는 피부 미백 물질 스크리닝 방법을 개시한다. 또한 본 발명은 상기 피부 미백 물질 스크리닝 방법에 따라 스크리닝된 물질을 유효 성분으로 포함하는 피부 미백용 조성물을 개시한다.
Abstract:
La présente invention a pour objet sur un procédé in vitro ou ex vivo pour la conservation et/ou la maintenance en survie d'un épiderme. Sous un autre aspect, la présente invention comprend le modèle d'épiderme ainsi obtenu et sur son utilisation dans un kit ou dans procédé pour le criblage ou la sélection de composés cosmétiques ou pharmaceutiques.
Abstract:
The present invention features a method for producing a dedifferentiated melanocyte. The method involves contacting a melanocyte with an agent that activates Notchl and selecting for a dedifferentiated melanocyte which exhibits a premelanoma stem cell-like state. Agents for activating Notchl and methods for using the cells and agents are also provided.
Abstract:
The present invention relates to dermal papilla-specific markers and a method for isolating dermal papilla cells using the same. Dermal papilla cells isolated in accordance with the method of the invention and treated with BMP6 are useful for promoting hair growth.
Abstract:
The present invention in general relates to the field of eukaryotic cell culture and tissue engineering, more closely to a method for the cultivation of eukaryotic cells and the use of cells cultured according to this method for the preparation of cell transplants e.g. autologous cell transplants. In particular, the invention relates to the use of keratinocyte and/or melanocyte and/or fibroblast transplants prepared according to the present invention for the treatment of various dermatological symptoms and diseases. The invention further relates to keratinocyte and/or melanocyte and/or fibroblast transplants, e.g. cell culture preparations, prepared according to the present invention. The invention concerns areas involving eukaryotic cell culture and tissue engineering, particularly fibrin-containing keratinocyte, melanocyte and fibroblast suspensions based on three constituents. The invention is particularly useful in the treatment of various conditions of skin, e.g. chronic ulcers of different origins, burn wounds, post-traumatic wound defects, defects of extensive, benign, naevoid skin changes and of scars after prior dermabrasion, vitiligo, piebaldism, etc.
Abstract:
Isolated apoptotic epithelial scaffolds are disclosed The scaffolds find use in promoting re-vascularization of wounded tissues Provided are isolated or ex vivo trl-layered cell sorted tissues which are comprised of a bottom layer comprised of an apoptotic epithelial scaffold a middle layer comprised of mesenchymal cells, and a top layer comprised of non-differentiated epithelial cells In vitro methods are disclosed for generating isolated or ex vivo tri-layered cell sorted tissues by exposing cultured mixtures of epithelial and mesenchymal cells to an inflammatory cytokine Further provided are methods of promoting wound healing and re-vasculaπzation of wounds by contacting wounded tissue with the apoptotic epithelial scaffolds