公开(公告)号：WO2013127921A1

公开(公告)日：2013-09-06

申请号：PCT/EP2013/054030

申请日：2013-02-28

Applicant: TECHNISCHE UNIVERSITÄT BERLIN

Inventor： ROSOWSKI, Mark ,  FATEHI-VARKANI, Shirin ,  LAUSTER, Roland ,  MARX, Uwe

IPC: C12N5/077 ,  G01N33/50 ,  C12N5/0789 ,  C12N5/071

CPC classification number: C12N5/0697 ,  A61L27/10 ,  A61L27/3834 ,  A61L27/3847 ,  A61L27/3852 ,  A61L27/50 ,  A61L27/54 ,  A61L2300/64 ,  A61L2430/02 ,  A61L2430/06 ,  C12M21/08 ,  C12M29/00 ,  C12N5/0647 ,  C12N5/0655 ,  C12N5/0663 ,  C12N2502/1171 ,  C12N2502/1311 ,  C12N2502/1317 ,  C12N2502/1358 ,  C12N2506/1353 ,  C12N2513/00 ,  C12N2533/14 ,  G01N33/502 ,  G01N33/5044 ,  G01N33/5088 ,  G01N33/6887

Abstract: The present invention is directed to a 3D in vitro bi-phasic cartilage-bone organoid comprising: a) a layer of artificial cartilage tissue; and b) a layer of artificial bone tissue, wherein the artificial bone tissue comprises a structure-giving scaffold and a bone marrow structure; wherein the layer of artificial cartilage tissue contacts at least one surface of the layer of artificial bone tissue, as well as to uses and a method of production thereof.

Abstract translation: 本发明涉及3D体外双相软骨 - 骨组织，其包括：a）人造软骨组织层; 和b）一层人造骨组织，其中所述人造骨组织包括结构赋予支架和骨髓结构; 其中所述人造软骨组织层接触人造骨组织层的至少一个表面，以及其用途及其生产方法。

公开(公告)号：WO2013127671A1

公开(公告)日：2013-09-06

申请号：PCT/EP2013/053361

申请日：2013-02-20

Applicant: TECHNISCHE UNIVERSITÄT BERLIN

Inventor： LAUSTER, Roland ,  MARX, Uwe ,  BINDER, Jennifer ,  ROSOWSKI, Mark

IPC: A61L27/38

CPC classification number: C12N5/0654 ,  A61K35/32 ,  A61L27/3804 ,  A61L27/3821 ,  A61L27/3865 ,  A61L2430/12 ,  C12N5/0664 ,  C12N2502/094 ,  C12N2506/1361 ,  C12N2513/00 ,  G01N33/5044 ,  G01N33/6887

Abstract: The present invention is directed to a method of preparing an artificial tooth primordium in vitro, comprising the steps: a) providing isolated mesenchymal dental pulp cells; and b) culturing the mesenchymal dental pulp cells under non-adherent conditions to form a cell aggregate representing an artificial tooth primordium; as well as to an artificial tooth primordium derived therefrom.

Abstract translation: 本发明涉及一种在体外制备人造牙原基的方法，包括以下步骤：a）提供分离的间充质牙髓细胞; 和b）在非粘附条件下培养间充质牙髓细胞以形成代表人造牙原基的细胞聚集体; 以及由其衍生的人造牙齿原基。

公开(公告)号：WO2012016711A1

公开(公告)日：2012-02-09

申请号：PCT/EP2011/003940

申请日：2011-08-05

Applicant: TECHNISCHE UNIVERSITÄT BERLIN ,  MARX, Uwe ,  LINDNER, Gerd ,  HORLAND, Reyk ,  HOFFMANN, Silke ,  LAUSTER, Roland

Inventor： MARX, Uwe ,  LINDNER, Gerd ,  HORLAND, Reyk ,  HOFFMANN, Silke ,  LAUSTER, Roland

IPC: C12M3/06 ,  C12N5/07

CPC classification number: C12M21/08 ,  C12M23/16 ,  C12M25/10 ,  C12M25/14 ,  C12M29/08 ,  C12M29/16 ,  C12N5/0691

Abstract: This invention concerns a self-contained circulation system, which supports the formation of capillaries in capillary growth sections and allows the formation of micro organoids and/or micro tissue sections for monitoring the effect of one or more test compounds and determining efficacy, side-effects, biosafety, metabolites, mode of action or organ regeneration as well as methods of establishing such micro organoids and/or micro tissue in said self-contained circulation system.

Abstract translation: 本发明涉及一种独立的循环系统，其支持在毛细血管生长部分中形成毛细管并且允许形成微组织和/或微组织切片以监测一种或多种测试化合物的作用并确定功效，副作用 ，生物安全性，代谢物，作用方式或器官再生，以及在所述自给式循环系统中建立这种微有机物和/或微组织的方法。

公开(公告)号：WO2009118283A1

公开(公告)日：2009-10-01

申请号：PCT/EP2009/053363

申请日：2009-03-23

Applicant: TECHNISCHE UNIVERSITÄT BERLIN ,  LINDNER, Gerd ,  LAUSTER, Roland

Inventor： LINDNER, Gerd ,  LAUSTER, Roland

IPC: C12N5/08 ,  A61K35/36 ,  A61P17/14

CPC classification number: A61L27/60 ,  A61K35/12 ,  A61L27/3604 ,  A61L27/3804 ,  A61L27/3869 ,  A61L27/3886 ,  A61L2430/18 ,  C12N5/0627 ,  C12N5/0698 ,  C12N2502/092 ,  C12N2502/094 ,  C12N2502/1323 ,  C12N2533/52 ,  C12N2533/54

Abstract: The present invention relates to a method for producing hair microfollicles comprising the steps of: a) providing de novopapillae, b) providing other cell populations selected from the group of fibroblasts, keratinocytes and melanocytes, and co-culturing the de novopapillae with at least one other cell population in non-adherent culture vessels.The present invention relates also to methods of producing de novo papillae usable in said method for producing hair microfollicles.

Abstract translation: 本发明涉及一种生产毛发微生物的方法，包括以下步骤：a）提供去痘病毒，b）提供从成纤维细胞，角质形成细胞和黑素细胞组中选出的其它细胞群，并与去痘痘科共同培养至少一种 非贴壁培养容器中的其它细胞群体。本发明还涉及可用于所述生产毛发微生物的方法的从头乳头的生产方法。