摘要:
The subject invention concerns materials and methods for providing plants or plant tissue with increased resistance to heat conditions and/or increased starch biosynthesis. Increased resistance of a plant or plant tissue to heat conditions provides for decreased yield losses as compared to the yield losses generally observed at elevated temperatures. One aspect of the invention concerns polynucleotides that encode a mutant plant small subunit of AGP ase. The subject invention also comprises a mutant plant small subunit of AGP ase encoded by a polynucleotide of the invention. The subject invention also concerns plants comprising a polynucleotide of the invention and method for making the plants.
摘要:
Compositions and methods for increasing heat resistance or starch biosynthesis in plants are provided herein. Polynucleotides, polypeptides, and expression constructs for expressing mutant AGPase subunit proteins, plants comprising the polynucleotides, polypeptides or expression constructs, and methods of producing transgenic plants are also provided.
摘要:
The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.
摘要:
The subject invention concerns materials and methods for providing plants or plant tissue with increased resistance to heat conditions and/or increased starch biosynthesis. Increased resistance of a plant or plant tissue to heat conditions provides for decreased yield losses as compared to the yield losses generally observed at elevated temperatures. One aspect of the invention concerns polynucleotides that encode a mutant plant small subunit of AGP ase. The subject invention also comprises a mutant plant small subunit of AGP ase encoded by a polynucleotide of the invention. The subject invention also concerns plants comprising a polynucleotide of the invention and method for making the plants.
摘要:
The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.
摘要:
Materials and methods for enhancing heat stability of AGPase by mutating amino acids within the large subunit of AGPase are disclosed. Polynucleotides that encode a mutant large subunit of AGPase provide for increased heat stability when expressed with AGPase small subunit. In one embodiment, the mutant large subunit has a single amino acid mutation. Exemplified mutant large subunits include those of SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO:16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, and SEQ ID NO:52. In further embodiments, the mutant large subunit comprises two or more amino acid mutations. An exemplified mutant large subunit comprising two or more amino acid mutations is designated as SH2N131R:C424V having an amino acid sequence shown in SEQ ID NO:23. In another exemplified embodiment, a mutant large subunit of the invention, designated herein as SH2-E, comprises 8 amino acid mutations.
摘要翻译:公开了通过在AGPase的大亚基内突变氨基酸来增强AGPase的热稳定性的材料和方法。 编码AGPase的突变体大亚基的多核苷酸提供当用AGPase小亚基表达时增加的热稳定性。 在一个实施方案中,突变体大亚基具有单个氨基酸突变。 示例性突变体大亚基包括SEQ ID NO:2,SEQ ID NO:3,SEQ ID NO:4,SEQ ID NO:5,SEQ ID NO:6,SEQ ID NO:7,SEQ ID NO:8,SEQ ID NO: SEQ ID NO:9,SEQ ID NO:10,SEQ ID NO:11,SEQ ID NO:12,SEQ ID NO:13,SEQ ID NO:14,SEQ ID NO:15,SEQ ID NO:16, :17,SEQ ID NO:18,SEQ ID NO:19,SEQ ID NO:20,SEQ ID NO:21,SEQ ID NO:22,SEQ ID NO:23,SEQ ID NO:25,SEQ ID NO:27 ,和SEQ ID NO:52。 在另外的实施方案中,突变体大亚基包含两个或多个氨基酸突变。 包含两个或多个氨基酸突变的示例性突变体大亚基称为具有SEQ ID NO:23所示氨基酸序列的SH2N131R:C424V。 在另一示例性实施方案中,本发明的突变体大亚基(本文称为SH2-E)包含8个氨基酸突变。
摘要:
The subject invention concerns materials and methods for providing plants or plant tissue with increased resistance to heat conditions and/or increased starch biosynthesis. Increased resistance of a plant or plant tissue to heat conditions provides for decreased yield losses as compared to the yield losses generally observed at elevated temperatures. One aspect of the invention concerns polynucleotides that encode a mutant plant small subunit of AGP ase. The subject invention also comprises a mutant plant small subunit of AGP ase encoded by a polynucleotide of the invention. The subject invention also concerns plants comprising a polynucleotide of the invention and method for making the plants.
摘要:
La presente invención describe una serie de compuestos útiles para reducir o anular procesos determinantes de la patogenicidad y virulencia bacterianas y de la adherencia bacteriana a superficies inertes o células tales como la producción de adhesinas, la motilidad flagelar y la formación de EPSs y biofilms bacterianos. Estos compuestos pueden usarse para la elaboración de composiciones farmacéuticas antibacterianas o de composiciones antisépticas para el tratamiento de un amplio abanico de infecciones bacterianas, como por ejemplo, E. coli, S. typhi, S. dysenteteriae, V. chlolerae, P. aeruginosa, H. pylori, L. monocytogenes, C. difficile y S. pyogenes. También describe una serie de compuestos que favorecen la uníon entre rhizobacterias beneficiosas y células de las raíces de las plantas. Además, se describe un procedimiento para la identificación de dichos compuestos.
摘要:
This invention is intended to allow accumulation of large quantities of soluble sugars in tissue other than plant seeds. A plant is modified so as to suppress a gene encoding a subunit exhibiting the highest sequence similarity with the subunit encoded by the AGPL1 gene of rice among subunits constituting.
摘要:
The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.