HEAT RESISTANT PLANTS AND PLANT TISSUES AND METHODS AND MATERIALS FOR MAKING AND USING SAME
    1.
    发明申请
    HEAT RESISTANT PLANTS AND PLANT TISSUES AND METHODS AND MATERIALS FOR MAKING AND USING SAME 审中-公开
    耐热植物和植物组织及其制备和使用它们的方法和材料

    公开(公告)号:WO2009126208A3

    公开(公告)日:2010-03-18

    申请号:PCT/US2009001903

    申请日:2009-03-26

    摘要: The subject invention concerns materials and methods for providing plants or plant tissue with increased resistance to heat conditions and/or increased starch biosynthesis. Increased resistance of a plant or plant tissue to heat conditions provides for decreased yield losses as compared to the yield losses generally observed at elevated temperatures. One aspect of the invention concerns polynucleotides that encode a mutant plant small subunit of AGP ase. The subject invention also comprises a mutant plant small subunit of AGP ase encoded by a polynucleotide of the invention. The subject invention also concerns plants comprising a polynucleotide of the invention and method for making the plants.

    摘要翻译: 本发明涉及为植物或植物组织提供增加的耐热条件和/或增加的淀粉生物合成的材料和方法。 与通常在升高的温度下观察到的产量损失相比,植物或植物组织对热条件的增加的抗性提供降低的产量损失。 本发明的一个方面涉及编码AGP抗体突变体植物小亚基的多核苷酸。 本发明还包含由本发明的多核苷酸编码的AGP的突变体植物小亚基。 本发明还涉及包含本发明的多核苷酸和用于制备植物的方法的植物。

    METHODS FOR INCREASING GRAIN YIELD
    2.
    发明申请
    METHODS FOR INCREASING GRAIN YIELD 审中-公开
    增加谷物产量的方法

    公开(公告)号:WO2015179191A2

    公开(公告)日:2015-11-26

    申请号:PCT/US2015030604

    申请日:2015-05-13

    申请人: UNIV FLORIDA

    IPC分类号: C12N9/12 C12N15/82

    摘要: Compositions and methods for increasing heat resistance or starch biosynthesis in plants are provided herein. Polynucleotides, polypeptides, and expression constructs for expressing mutant AGPase subunit proteins, plants comprising the polynucleotides, polypeptides or expression constructs, and methods of producing transgenic plants are also provided.

    摘要翻译: 本文提供了增加植物耐热性或淀粉生物合成的组合物和方法。 还提供了用于表达突变型AGP酶亚基蛋白的多核苷酸,多肽和表达构建体,包含多核苷酸,多肽或表达构建体的植物,以及产生转基因植物的方法。

    HEAT STABLE MUTANTS OF STARCH BIOSYNTHESIS ENZYMES
    3.
    发明申请
    HEAT STABLE MUTANTS OF STARCH BIOSYNTHESIS ENZYMES 审中-公开
    淀粉生物素酶的热稳定突变体

    公开(公告)号:WO2003070901A2

    公开(公告)日:2003-08-28

    申请号:PCT/US2003/004781

    申请日:2003-02-18

    IPC分类号: C12N

    摘要: The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.

    摘要翻译: 本发明涉及编码具有增加的热稳定性的酶的新型突变体多核苷酸分子。 当在植物中表达时,这些多核苷酸导致在热胁迫条件下生长的植物中的产量增加。 本发明的多核苷酸分子编码玉米胚乳ADP葡萄糖焦磷酸化酶(AGP)和可溶性淀粉合成酶(SSS)酶活性。 繁殖含有突变体多核苷酸或转化了突变多核苷酸并表达多核苷酸编码的多肽的植物和植物组织也被本发明考虑。 本发明还涉及分离本发明范围内考虑的多核苷酸和多肽的方法。 还提供了在热胁迫条件下生长的植物中提高产量的方法。

    HEAT RESISTANT PLANTS AND PLANT TISSUES AND METHODS AND MATERIALS FOR MAKING AND USING SAME
    4.
    发明申请
    HEAT RESISTANT PLANTS AND PLANT TISSUES AND METHODS AND MATERIALS FOR MAKING AND USING SAME 审中-公开
    耐热植物和植物组织及其制备和使用方法和材料

    公开(公告)号:WO2009126208A8

    公开(公告)日:2010-11-18

    申请号:PCT/US2009001903

    申请日:2009-03-26

    摘要: The subject invention concerns materials and methods for providing plants or plant tissue with increased resistance to heat conditions and/or increased starch biosynthesis. Increased resistance of a plant or plant tissue to heat conditions provides for decreased yield losses as compared to the yield losses generally observed at elevated temperatures. One aspect of the invention concerns polynucleotides that encode a mutant plant small subunit of AGP ase. The subject invention also comprises a mutant plant small subunit of AGP ase encoded by a polynucleotide of the invention. The subject invention also concerns plants comprising a polynucleotide of the invention and method for making the plants.

    摘要翻译: 本发明涉及用于向植物或植物组织提供对热条件和/或增加的淀粉生物合成的抗性增加的材料和方法。 与通常在升高的温度下观察到的产量损失相比,植物或植物组织对加热条件的抗性增加提供了降低的产量损失。 本发明的一个方面涉及编码AGP酶的突变植物小亚基的多核苷酸。 本发明还包括由本发明的多核苷酸编码的AGP酶的突变植物小亚基。 本发明还涉及包含本发明多核苷酸的植物和制备该植物的方法。

    HEAT STABLE MUTANTS OF STARCH BIOSYNTHESIS ENZYMES
    5.
    发明申请
    HEAT STABLE MUTANTS OF STARCH BIOSYNTHESIS ENZYMES 审中-公开
    淀粉生物素酶的热稳定突变体

    公开(公告)号:WO9958698A3

    公开(公告)日:2000-04-06

    申请号:PCT/US9910716

    申请日:1999-05-14

    申请人: UNIV FLORIDA

    摘要: The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.

    摘要翻译: 本发明涉及编码具有增加的热稳定性的酶的新型突变体多核苷酸分子。 当在植物中表达时,这些多核苷酸导致在热胁迫条件下生长的植物中的产量增加。 本发明的多核苷酸分子编码玉米胚乳ADP葡萄糖焦磷酸化酶(AGP)和可溶性淀粉合成酶(SSS)酶活性。 繁殖含有突变体多核苷酸或转化了突变多核苷酸并表达多核苷酸编码的多肽的植物和植物组织也被本发明考虑。 本发明还涉及分离本发明范围内考虑的多核苷酸和多肽的方法。 还提供了在热胁迫条件下生长的植物中提高产量的方法。

    MATERIALS AND METHODS FOR ENHANCED HEAT STABILITY AND KINETIC PARAMETERS OF ADP-GLUCOSE PYROPHOSPHORYLASE
    6.
    发明申请
    MATERIALS AND METHODS FOR ENHANCED HEAT STABILITY AND KINETIC PARAMETERS OF ADP-GLUCOSE PYROPHOSPHORYLASE 审中-公开
    ADP-GLUCOSE PYROPHOSPHORYLASE的增强热稳定性和动力学参数的材料与方法

    公开(公告)号:WO2015013444A1

    公开(公告)日:2015-01-29

    申请号:PCT/US2014/047879

    申请日:2014-07-23

    IPC分类号: C12N15/54 C12N15/82 A01H5/00

    摘要: Materials and methods for enhancing heat stability of AGPase by mutating amino acids within the large subunit of AGPase are disclosed. Polynucleotides that encode a mutant large subunit of AGPase provide for increased heat stability when expressed with AGPase small subunit. In one embodiment, the mutant large subunit has a single amino acid mutation. Exemplified mutant large subunits include those of SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO:16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, and SEQ ID NO:52. In further embodiments, the mutant large subunit comprises two or more amino acid mutations. An exemplified mutant large subunit comprising two or more amino acid mutations is designated as SH2N131R:C424V having an amino acid sequence shown in SEQ ID NO:23. In another exemplified embodiment, a mutant large subunit of the invention, designated herein as SH2-E, comprises 8 amino acid mutations.

    摘要翻译: 公开了通过在AGPase的大亚基内突变氨基酸来增强AGPase的热稳定性的材料和方法。 编码AGPase的突变体大亚基的多核苷酸提供当用AGPase小亚基表达时增加的热稳定性。 在一个实施方案中,突变体大亚基具有单个氨基酸突变。 示例性突变体大亚基包括SEQ ID NO:2,SEQ ID NO:3,SEQ ID NO:4,SEQ ID NO:5,SEQ ID NO:6,SEQ ID NO:7,SEQ ID NO:8,SEQ ID NO: SEQ ID NO:9,SEQ ID NO:10,SEQ ID NO:11,SEQ ID NO:12,SEQ ID NO:13,SEQ ID NO:14,SEQ ID NO:15,SEQ ID NO:16, :17,SEQ ID NO:18,SEQ ID NO:19,SEQ ID NO:20,SEQ ID NO:21,SEQ ID NO:22,SEQ ID NO:23,SEQ ID NO:25,SEQ ID NO:27 ,和SEQ ID NO:52。 在另外的实施方案中,突变体大亚基包含两个或多个氨基酸突变。 包含两个或多个氨基酸突变的示例性突变体大亚基称为具有SEQ ID NO:23所示氨基酸序列的SH2N131R:C424V。 在另一示例性实施方案中,本发明的突变体大亚基(本文称为SH2-E)包含8个氨基酸突变。

    HEAT RESISTANT PLANTS AND PLANT TISSUES AND METHODS AND MATERIALS FOR MAKING AND USING SAME
    7.
    发明申请
    HEAT RESISTANT PLANTS AND PLANT TISSUES AND METHODS AND MATERIALS FOR MAKING AND USING SAME 审中-公开
    耐热植物和植物组织及其制备和使用它们的方法和材料

    公开(公告)号:WO2009126208A2

    公开(公告)日:2009-10-15

    申请号:PCT/US2009/001903

    申请日:2009-03-26

    摘要: The subject invention concerns materials and methods for providing plants or plant tissue with increased resistance to heat conditions and/or increased starch biosynthesis. Increased resistance of a plant or plant tissue to heat conditions provides for decreased yield losses as compared to the yield losses generally observed at elevated temperatures. One aspect of the invention concerns polynucleotides that encode a mutant plant small subunit of AGP ase. The subject invention also comprises a mutant plant small subunit of AGP ase encoded by a polynucleotide of the invention. The subject invention also concerns plants comprising a polynucleotide of the invention and method for making the plants.

    摘要翻译: 本发明涉及为植物或植物组织提供增加的耐热条件和/或增加的淀粉生物合成的材料和方法。 与通常在升高的温度下观察到的产量损失相比,植物或植物组织对热条件的增加的抗性提供降低的产量损失。 本发明的一个方面涉及编码AGP抗体突变体植物小亚基的多核苷酸。 本发明还包含由本发明的多核苷酸编码的AGP的突变体植物小亚基。 本发明还涉及包含本发明的多核苷酸和用于制备植物的方法的植物。

    COMPUESTOS ÚTILES PARA EL TRATAMIENTO DE INFECCIONES BACTERIANAS, COMPOSICIONES FARMACÉUTICAS QUE LOS CONTIENEN, PROCEDIMIENTO DE IDENTIFICACIÓN DE LOS MISMOS Y SUS APLICACIONES
    8.
    发明申请
    COMPUESTOS ÚTILES PARA EL TRATAMIENTO DE INFECCIONES BACTERIANAS, COMPOSICIONES FARMACÉUTICAS QUE LOS CONTIENEN, PROCEDIMIENTO DE IDENTIFICACIÓN DE LOS MISMOS Y SUS APLICACIONES 审中-公开
    用于治疗细菌感染的化合物,含有化合物的药物组合物,其鉴定方法及其用途

    公开(公告)号:WO2014102432A1

    公开(公告)日:2014-07-03

    申请号:PCT/ES2013/070925

    申请日:2013-12-24

    IPC分类号: C12Q1/68

    摘要: La presente invención describe una serie de compuestos útiles para reducir o anular procesos determinantes de la patogenicidad y virulencia bacterianas y de la adherencia bacteriana a superficies inertes o células tales como la producción de adhesinas, la motilidad flagelar y la formación de EPSs y biofilms bacterianos. Estos compuestos pueden usarse para la elaboración de composiciones farmacéuticas antibacterianas o de composiciones antisépticas para el tratamiento de un amplio abanico de infecciones bacterianas, como por ejemplo, E. coli, S. typhi, S. dysenteteriae, V. chlolerae, P. aeruginosa, H. pylori, L. monocytogenes, C. difficile y S. pyogenes. También describe una serie de compuestos que favorecen la uníon entre rhizobacterias beneficiosas y células de las raíces de las plantas. Además, se describe un procedimiento para la identificación de dichos compuestos.

    摘要翻译: 本发明涉及一系列用于减少或消除确定细菌毒力和致病性以及对惰性表面或细胞的细菌粘附的方法的化合物,例如粘附素的产生,鞭毛运动以及细菌生物膜和外多糖的形成。 所述化合物可用于生产用于治疗广泛细菌感染的抗菌药物组合物或防腐组合物,例如大肠杆菌,伤寒沙门氏菌,痢疾杆菌,霍乱弧菌,P. 铜绿假单胞菌,幽门螺杆菌,单核细胞增生李斯特氏菌,艰难梭菌和化脓性链球菌。 本发明还涉及促进有益根际细菌与植物根系细胞之间的结合的一系列化合物。 此外,本发明涉及鉴定所述化合物的方法。

    HEAT STABLE MUTANTS OF STARCH BIOSYNTHESIS ENZYMES
    10.
    发明申请
    HEAT STABLE MUTANTS OF STARCH BIOSYNTHESIS ENZYMES 审中-公开
    淀粉生物素酶的热稳定突变体

    公开(公告)号:WO99058698A2

    公开(公告)日:1999-11-18

    申请号:PCT/US1999/010716

    申请日:1999-05-14

    摘要: The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.

    摘要翻译: 本发明涉及编码具有增加的热稳定性的酶的新型突变体多核苷酸分子。 当在植物中表达时,这些多核苷酸导致在热胁迫条件下生长的植物中的产量增加。 本发明的多核苷酸分子编码玉米胚乳ADP葡萄糖焦磷酸化酶(AGP)和可溶性淀粉合成酶(SSS)酶活性。 繁殖含有突变体多核苷酸或转化了突变多核苷酸并表达多核苷酸编码的多肽的植物和植物组织也被本发明考虑。 本发明还涉及分离本发明范围内考虑的多核苷酸和多肽的方法。 还提供了在热胁迫条件下生长的植物中提高产量的方法。