METHODS OF MAKING FUNGUS ORGANISM HYBRIDS WITH NOVEL OR ENHANCED PHYTOCHEMICALS

    公开(公告)号:WO2023091721A1

    公开(公告)日:2023-05-25

    申请号:PCT/US2022/050496

    申请日:2022-11-18

    Inventor: TUDELA, Jerred

    Abstract: Methods of crossbreeding dikaryotic fungus organisms to yield a hybrid fungus organism with a novel or enhanced phytochemical. The methods include providing a first growth medium; placing a first dikaryotic fungus organism with a first phytochemical on the first growth medium; placing a second dikaryotic fungus organism with a second phytochemical on the first growth medium adjacent to the first dikaryotic fungus organism; allowing the first dikaryotic fungus organism to replicate and to form a first colony on the first growth medium; allowing the second dikaryotic fungus organism to replicate and to form a second colony on the first growth medium; and allowing the first colony and the second colony to expand until they intersect along a clamp line where the first colony and the second colony exchange genetic material between them to yield a hybrid fungus organism with a novel or enhanced phytochemical.

    Type I-C CRISPR-Cas3系统及其应用
    10.
    发明申请

    公开(公告)号:WO2022253277A1

    公开(公告)日:2022-12-08

    申请号:PCT/CN2022/096648

    申请日:2022-06-01

    Abstract: 涉及规律成簇的间隔短回文重复(CRISPR)技术领域。提供了用于核酸编辑(例如,基因或基因组编辑,大片段缺失,单碱基编辑,基因组结构变异)的方法以及组合物,包括使用Type I-C CRISPR-Cas3系统。Type I-C CRISPR-Cas3系统可以实现基因组的精确的大片段缺失,例如,对于单个基因编码框任意长度的敲除,基因调控元件如长的lncRNA或者增强子的敲除,以及实现基因或基因组的单碱基编辑和基因组大的结构变异。

Patent Agency Ranking