公开(公告)号：WO2014028772A2

公开(公告)日：2014-02-20

申请号：PCT/US2013/055198

申请日：2013-08-15

Applicant: BANGLADESH JUTE RESEARCH INSTITUTE ,  ALAM, Maqsudul

Inventor： ALAM, Maqsudul ,  ISLAM, Mohammed, Shahidul ,  HOSSEN, Mohammed, Mosaddeque ,  HAQUE, Mohammed, Samiul ,  ALAM, Mohammed, Monjurul

IPC: C12N9/88

CPC classification number: C12N9/88 ,  C12N9/18 ,  C12N9/2405 ,  C12Y301/01011 ,  C12Y402/02002 ,  C12Y402/02023

Abstract: The present invention discloses isolated polynucleotide encoding enzymes, derived from the fungus Macrophomina phaseolina ("M. phaseolina"), responsible for degrading pectin, and it comprises and/or consists of nucleotide sequences set forth in SEQ. ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58 and 61, or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60 and 63; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, with or having enhanced production of pectin degrading enzyme. The polypeptide of the invention can be used for, amongst other things, manufactured fruit juice, textile products, pulp and paper, coffee, tea and oil extraction and pectic waste water treatment.

Abstract translation: 本发明公开了编码负责降解果胶的真菌Macmatomina phaseolina（“M. phaseolina”）的分离的多核苷酸，并且其包含和/或由下列核苷酸序列组成： 序列。 ID号2,5,8,11,14,17,20,23,26,29,32,35,38,41,44,47,50,53,56,59,62,1，4,7 ，10,13,16,19,22,25,28,31,34,37,40,43,46,49,52,55,58和61或这些序列的互补序列。 本发明还涉及分离的多肽，其由SEQ ID No.3,6,9,12,15,18,21,24,27,30,33,36,39,42,45， 48，51，54，57，60和63; 包含该多核苷酸的重组基因构建体; 包含该重组基因构建体的转化体和转基因真菌，具有或提高了果胶降解酶的产生。 本发明的多肽可用于生产果汁，纺织品，纸浆和纸，咖啡，茶和石油提取以及果汁废水处理等。

公开(公告)号：WO2014028772A3

公开(公告)日：2014-05-08

申请号：PCT/US2013055198

申请日：2013-08-15

Applicant: BANGLADESH JUTE RES INST

Inventor： ISLAM MOHAMMED SHAHIDUL ,  HOSSEN MOHAMMED MOSADDEQUE ,  HAQUE MOHAMMED SAMIUL ,  ALAM MOHAMMED MONJURUL ,  ALAM MAQSUDUL

IPC: C12N9/88 ,  C12P19/34 ,  D06M16/00

CPC classification number: C12N9/88 ,  C12N9/18 ,  C12N9/2405 ,  C12Y301/01011 ,  C12Y402/02002 ,  C12Y402/02023

Abstract: The present invention discloses isolated polynucleotide encoding enzymes, derived from the fungus Macrophomina phaseolina ("M. phaseolina"), responsible for degrading pectin, and it comprises and/or consists of nucleotide sequences set forth in SEQ. ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58 and 61, or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60 and 63; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, with or having enhanced production of pectin degrading enzyme. The polypeptide of the invention can be used for, amongst other things, manufactured fruit juice, textile products, pulp and paper, coffee, tea and oil extraction and pectic waste water treatment.

Abstract translation: 本发明公开了分离的多核苷酸编码酶，该多核苷酸来自真菌巨噬细胞相容素（“M. phaseolina”），其负责降解果胶，并且其包含和/或由SEQ ID NO：1所示的核苷酸序列组成。 编号2，5，8，11，14，17，20，23，26，29，32，35，38，41，44，47，50，53，56，59，62，1,4,7 ，10,13,16,19,22,25,28,31,34,37,40,43,46,49,52,55,58和61，或这些序列的补体。 本发明还涉及由SEQ ID No.3,6,9,12,15,18,21,24,27,30,33,36,39,42,45， 48，51，54，57，60及63条; 包含该多核苷酸的重组基因构建体; 转化体和转基因真菌，其包含具有或具有增强的果胶降解酶生产的重组基因构建体。 本发明的多肽可以用于制造果汁，纺织品，纸浆和纸，咖啡，茶和油提取和果胶废水处理。

公开(公告)号：WO2013079828A1

公开(公告)日：2013-06-06

申请号：PCT/FR2012/052054

申请日：2012-09-14

Applicant: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE - CNRS - ,  UNIVERSITÉ PIERRE ET MARIE CURIE ,  CENTRE D'ÉTUDE ET DE VALORISATION DES ALGUES ,  COLLEN, Pi ,  HELBERT, William ,  LERAT, Yannick ,  SASSI, Jean-François

Inventor： COLLEN, Pi ,  HELBERT, William ,  LERAT, Yannick ,  SASSI, Jean-François

IPC: C12N9/88

CPC classification number: C12N9/2402 ,  C12N9/88 ,  C12P19/00 ,  C12Y402/02023 ,  C12Y402/02024

Abstract: La présente invention se rapporte notamment à des protéines, à des séquences d'acides nucléiques codant pour ces protéines, à des vecteurs comprenant ces séquences codantes, à un procédé de fabrication de ces protéines, ainsi qu'à un procédé d'hydrolyse d'oligosaccharides utilisant ces protéines. En particulier, elle concerne la protéine de séquence SEQ ID n°1. La présente invention trouve des applications dans la valorisation des bio-ressources naturelles constituées par les organismes et les microorganismes comprenant des ulvanes, notamment les algues vertes.

Abstract translation: 本发明特别涉及蛋白质，编码相同的核酸序列，包含所述编码序列的载体，所述蛋白质的制备方法和使用其的寡糖水解方法。 特别地，本发明涉及序列SEQ ID NO： 本发明可以应用于生物和微生物形成的生物天然资源的回收，包括乌兰，特别是绿藻。