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    NUCLEOTIDE SEQUENCE ENCODING HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT22 (HD-ZIP PROTEIN 22) FROM CORCHORUS OLITORIUS AND CORCHORUS CAPSULARIS AND METHODS OF USE
    1.
    发明申请
    NUCLEOTIDE SEQUENCE ENCODING HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT22 (HD-ZIP PROTEIN 22) FROM CORCHORUS OLITORIUS AND CORCHORUS CAPSULARIS AND METHODS OF USE 审中-公开
    从CORCORUS OLITORIUS和CORCORUS CAPSULARIS编码家蚕皂苷拉链蛋白HAT22（HD-ZIP蛋白22）的核苷酸序列及其使用方法

    公开(公告)号：WO2015077538A8

    公开(公告)日：2015-07-16

    申请号：PCT/US2014066772

    申请日：2014-11-21

    Applicant: BANGLADESH JUTE RES INST ,  ALAM MAQSUDUL

    Inventor： ALAM MAQSUDUL ,  ISLAM MOHAMMED SHAHIDUL ,  AHMED BORHAN ,  HAQUE MOHAMMED SAMIUL ,  ALAM MOHAMMED MONJURUL

    IPC: A01H5/00 ,  C12N15/82

    CPC classification number: C12N15/8261 ,  C07K14/415 ,  Y02A40/146

    Abstract: The present invention relates to the isolated polynucleotide encoding homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22) from the plants Corchorus olitorius and Corchorus capsularis and corresponding polypeptide derived thereof. The present invention also relates to the plants having modulated expression of a nucleic acid encoding a homeobox-leucine zipper HAT22 polypeptide or a homologue thereof, which has the ability to modify, preferably to increase/enhance, the fiber length, plant height, and/or plant biomass. More specifically, the invention relates to polypeptides having homeobox-leucine zipper protein HAT22 activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. The present invention further provides vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22). The invention also provides methods for producing the said protein and methods for modifying the said protein in order to improve their desirable characteristics. The said protein of the invention can be used in a variety of ways, including increasing/enhancing the fiber length, height and biomass of plants and fiber yield.

    Abstract translation: 本发明涉及编码来自植物Corchorus olitorius和Corchorus capsularis的同源框 - 亮氨酸拉链蛋白HAT22（HD-ZIP蛋白22）的分离的多核苷酸及其衍生的相应多肽。 本发明还涉及具有调节表达编码同源框 - 亮氨酸拉链HAT22多肽或其同源物的核酸的植物，其具有修饰，优选增加/增强纤维长度，植物高度和/ 或植物生物量。 更具体地，本发明涉及具有同源异构体 - 亮氨酸拉链蛋白HAT22活性的多肽，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 本发明还提供载体，表达构建体和宿主细胞，其包含同源异构体 - 亮氨酸拉链蛋白HAT22（HD-ZIP蛋白22）的核苷酸序列和/或组成。 本发明还提供了用于生产所述蛋白质的方法和用于改变所述蛋白质的方法，以便改善其所需特征。 本发明的所述蛋白质可以以各种方式使用，包括增加/增强植物的纤维长度，高度和生物量以及纤维产量。

    NUCLEOTIDE SEQUENCE ENCODING HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT22 (HD-ZIP PROTEIN 22) FROM CORCHORUS OLITORIUS AND CORCHORUS CAPSULARIS AND METHODS OF USE
    3.
    发明申请
    NUCLEOTIDE SEQUENCE ENCODING HOMEOBOX-LEUCINE ZIPPER PROTEIN HAT22 (HD-ZIP PROTEIN 22) FROM CORCHORUS OLITORIUS AND CORCHORUS CAPSULARIS AND METHODS OF USE 审中-公开
    编码来自COROPORORUS OLITORIUS和CORCHORUS CAPSULARIS的homeobox-leucine ZIPPER蛋白HAT22（HD-ZIP蛋白22）的核苷酸序列和使用方法

    公开(公告)号：WO2015077538A3

    公开(公告)日：2015-09-03

    申请号：PCT/US2014066772

    申请日：2014-11-21

    Applicant: BANGLADESH JUTE RES INST ,  ALAM MAQSUDUL

    Inventor： ALAM MAQSUDUL ,  ISLAM MOHAMMED SHAHIDUL ,  AHMED BORHAN ,  HAQUE MOHAMMED SAMIUL ,  ALAM MOHAMMED MONJURUL

    IPC: C12N15/29 ,  C07K14/415

    CPC classification number: C12N15/8261 ,  C07K14/415 ,  Y02A40/146

    Abstract: The present invention relates to the isolated polynucleotide encoding homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22) from the plants Corchorus olitorius and Corchorus capsularis and corresponding polypeptide derived thereof. The present invention also relates to the plants having modulated expression of a nucleic acid encoding a homeobox-leucine zipper HAT22 polypeptide or a homologue thereof, which has the ability to modify, preferably to increase/enhance, the fiber length, plant height, and/or plant biomass. More specifically, the invention relates to polypeptides having homeobox-leucine zipper protein HAT22 activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. The present invention further provides vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the homeobox-leucine zipper protein HAT22 (HD-ZIP protein 22). The invention also provides methods for producing the said protein and methods for modifying the said protein in order to improve their desirable characteristics. The said protein of the invention can be used in a variety of ways, including increasing/enhancing the fiber length, height and biomass of plants and fiber yield.

    Abstract translation: 本发明涉及编码来自植物黄麻和黄麻的同源框 - 亮氨酸拉链蛋白HAT22（HD-ZIP蛋白22）的分离的多核苷酸及其衍生的相应多肽。 本发明还涉及具有调节的编码同源框 - 亮氨酸拉链HAT22多肽或其同系物的核酸表达的植物，其具有修饰，优选增加/增强纤维长度，植物高度和/ 或植物生物量。 更具体地，本发明涉及具有同源框 - 亮氨酸拉链蛋白HAT22活性的多肽，编码这些多肽的多核苷酸以及制备和使用这些多核苷酸和多肽的方法。 本发明进一步提供包含和/或由同源框 - 亮氨酸拉链蛋白HAT22（HD-ZIP蛋白22）的核苷酸序列组成的载体，表达构建体和宿主细胞。 本发明还提供了用于生产所述蛋白质的方法和用于修饰所述蛋白质以改进其期望特性的方法。 本发明的所述蛋白质可以以各种方式使用，包括增加/增强植物的纤维长度，高度和生物量以及纤维产量。

    PECTIN DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF
    4.
    发明申请
    PECTIN DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 审中-公开
    来自大蒜素的相关降解酶及其用途

    公开(公告)号：WO2014028772A3

    公开(公告)日：2014-05-08

    申请号：PCT/US2013055198

    申请日：2013-08-15

    Applicant: BANGLADESH JUTE RES INST

    Inventor： ISLAM MOHAMMED SHAHIDUL ,  HOSSEN MOHAMMED MOSADDEQUE ,  HAQUE MOHAMMED SAMIUL ,  ALAM MOHAMMED MONJURUL ,  ALAM MAQSUDUL

    IPC: C12N9/88 ,  C12P19/34 ,  D06M16/00

    CPC classification number: C12N9/88 ,  C12N9/18 ,  C12N9/2405 ,  C12Y301/01011 ,  C12Y402/02002 ,  C12Y402/02023

    Abstract: The present invention discloses isolated polynucleotide encoding enzymes, derived from the fungus Macrophomina phaseolina ("M. phaseolina"), responsible for degrading pectin, and it comprises and/or consists of nucleotide sequences set forth in SEQ. ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58 and 61, or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60 and 63; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, with or having enhanced production of pectin degrading enzyme. The polypeptide of the invention can be used for, amongst other things, manufactured fruit juice, textile products, pulp and paper, coffee, tea and oil extraction and pectic waste water treatment.

    Abstract translation: 本发明公开了分离的多核苷酸编码酶，该多核苷酸来自真菌巨噬细胞相容素（“M. phaseolina”），其负责降解果胶，并且其包含和/或由SEQ ID NO：1所示的核苷酸序列组成。 编号2，5，8，11，14，17，20，23，26，29，32，35，38，41，44，47，50，53，56，59，62，1,4,7 ，10,13,16,19,22,25,28,31,34,37,40,43,46,49,52,55,58和61，或这些序列的补体。 本发明还涉及由SEQ ID No.3,6,9,12,15,18,21,24,27,30,33,36,39,42,45， 48，51，54，57，60及63条; 包含该多核苷酸的重组基因构建体; 转化体和转基因真菌，其包含具有或具有增强的果胶降解酶生产的重组基因构建体。 本发明的多肽可以用于制造果汁，纺织品，纸浆和纸，咖啡，茶和油提取和果胶废水处理。

    LIGNIN DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF
    5.
    发明申请
    LIGNIN DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 审中-公开
    巨大芽孢杆菌木质素降解木质素酶及其用途

    公开(公告)号：WO2014028773A3

    公开(公告)日：2014-04-17

    申请号：PCT/US2013055199

    申请日：2013-08-15

    Applicant: BANGLADESH JUTE RES INST

    Inventor： ALAM MAQSUDUL ,  ISLAM MOHAMMED SHAHIDUL ,  HOSSEN MOHAMMED MOSADDEQUE ,  HAQUE MOHAMMED SAMIUL ,  ALAM MOHAMMED MONJURUL

    IPC: C12N9/08 ,  C07H21/04 ,  C07K14/37 ,  C12N15/53

    CPC classification number: C12N9/0061 ,  C12N9/0065

    Abstract: The present invention relates to the field of plant breeding and disease resistance in respect of lignin degradation of plant by Macrophomina phaseolina ("M. phaseolina"). The present invention discloses isolated polynucleotide encoding of the lignin degrading enzymes, produced by Macrophomina phaseolina, comprising and consisting of nucleotide sequence set forth in SEQ. ID Nos. 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 65, 68, 71, 74, 77, 80, 83, 86, 89, 92, 95, 98, 101, 104, 107, 110, 1, 4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58, 61, 64, 67, 70, 73, 76, 79, 82, 85, 88, 91, 94, 97, 100, 103, 106 and 109 or the complement of such sequences. The present invention also relates to isolated polypeptide encoded by the polynucleotide sequences set forth in SEQ ID Nos. 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60, 63, 66, 69, 72, 75, 78, 81, 84, 87, 90, 93, 96, 99, 102, 105, 108 and 111; a recombinant gene construct comprising the polynucleotide; a transformant and a transgenic fungus comprising the recombinant gene construct, having enhanced production of lignin degrading enzyme. The invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomerase to fermentable sugar in saccharification process of biomass.

    Abstract translation: 本发明涉及马麦角菌（Macmatomina phaseolina）（“M. phaseolina”）对植物木质素降解的植物育种和抗病性领域。 本发明公开了编码木质素降解酶的分离的多核苷酸，其由Macmatomina phaseolina产生，包含SEQ.ID.NO.1所示的核苷酸序列并由其组成。 ID号2，5，8，11，14，17，20，23，26，29，32，35，38，41，44，47，50，53，56，59，62，65，68，71 ，74，77，80，83，86，89，92，95，98，101，104，107，110，1，4，7，10，13，16，19，22，25，28，31，34 ，37，40，43，46，49，52，55，58，61，64，67，70，73，76，79，82，85，88，91，94，97，100，103，106和109 或这些序列的补充。 本发明还涉及一种由SEQ ID阐述的多核苷酸序列编码的分离的多肽，第3，6，9，12，15，18，21，24，27，30，33，36，39，42，45， 48,51,54,57,60,63,66,69,72,75,78,81,84,87,90,93,96,99,102,105,108和111;以及其中所述核苷酸序列与SEQ ID NO： 包含该多核苷酸的重组基因构建体; 包含该重组基因构建体的转化体和转基因真菌具有增强的木质素降解酶的产生。 本发明提供具有寡聚体酶活性的多肽，例如在生物质糖化过程中将难溶性可溶性寡聚体酶转化成可发酵糖的酶。

    CELLULOSE AND/OR HEMICELLULOSES DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF
    6.
    发明申请
    CELLULOSE AND/OR HEMICELLULOSES DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF 审中-公开
    纤维素和/或致细胞毒素降解大蒜素的酶及其用途

    公开(公告)号：WO2014028774A3

    公开(公告)日：2014-05-08

    申请号：PCT/US2013055200

    申请日：2013-08-15

    Applicant: BANGLADESH JUTE RES INST

    Inventor： ISLAM MOHAMMED SHAHIDUL ,  HOSSEN MOHAMMED MOSADDEQUE ,  HAQUE MOHAMMED SAMIUL ,  ALAM MOHAMMED MONJURUL ,  ALAM MAQSUDUL

    IPC: C12N9/42 ,  C12N15/00

    CPC classification number: C12N9/2402 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12N9/2471 ,  C12N9/2482 ,  C12N9/2494 ,  C12N9/88 ,  C12Y302/01037 ,  C12Y302/01051 ,  C12Y302/01055 ,  C12Y302/01059 ,  C12Y302/01075 ,  C12Y302/01089 ,  C12Y302/0113 ,  C12Y302/01177

    Abstract: The present invention provides nucleotide sequences of Macrophomina phaseolina ("M. phaseolina") that encodes proteins/enzymes with cellulolytic activity, including a cellulase activity, a endoglucanase, a cellobiohydrolase, a &bgr;-glucosidase, a a-glucosidase, a xylanase, a mannanse, a &bgr;-xylosidase, a a-xylosidase, a galactosidase, an arabinofuranosidase, a a- fucosidases, a &bgr;-galactanase, an unsaturated &bgr;-glucuronyl hydrolase and/or oligomerase activity. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes and methods for modifying the enzymes in order to improve their desirable characteristics. The enzymes of the invention can be used in a variety of, but not limited to, pharmaceutical, agricultural, food and feed processing, biofuel, energy efficiency and industrial contexts. These enzymes are also useful for complete hydrolysis of lignocellulosic biomass into simple sugar that can then be fermented to liquid fuels and chemical feedstocks.

    Abstract translation: 本发明提供了编码具有纤维素分解活性的蛋白质/酶的Macrophomina phaseolina（“M. phaseolina”）的核苷酸序列，包括纤维素酶活性，内切葡聚糖酶，纤维二糖水解酶，葡糖苷酶，α-葡糖苷酶，木聚糖酶， 甘露聚糖，α-木糖苷酶，α-木糖苷酶，半乳糖苷酶，阿拉伯呋喃糖苷酶，α-岩藻糖苷酶，β-半乳糖苷酶，不饱和的 - 葡萄糖醛酸水解酶和/或寡聚酶活性。 也提供了包含和/或由该酶基因的核苷酸序列组成的载体，表达构建体和宿主细胞。 本发明还提供了用于生产酶的方法和用于改变酶的方法，以便改善其期望的特性。 本发明的酶可以多种但不限于制药，农业，食品和饲料加工，生物燃料，能源效率和工业环境。 这些酶也可用于将木质纤维素生物质完全水解成单糖，然后将其发酵成液体燃料和化学原料。

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