公开(公告)号：WO2023002057A2

公开(公告)日：2023-01-26

申请号：PCT/EP2022/070715

申请日：2022-07-22

Applicant: INSTITUT PASTEUR ,  ECOLE POLYTECHNIQUE ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE

Inventor： BAROUD, Charles ,  COSSEC, Jack Christophe ,  DEJEAN, Anne ,  SART, Sébastien ,  TRABOULSI, Tatiana

IPC: C12N5/0735 ,  B01L3/00 ,  C12M3/06 ,  C12M1/12 ,  C12M3/00 ,  G01N21/00 ,  C12M23/16 ,  C12M25/14 ,  C12N2501/235 ,  C12N2501/40 ,  C12N2501/70 ,  C12N2501/72 ,  C12N2513/00 ,  C12N2535/00 ,  C12N5/0606

Abstract: The invention relates to in vitro generation of organized 3D cell structures recapitulating various degrees of early organogenesis, including head-trunk embryo-like structures, using epigenetic remodeling factors. The invention relates in particular to methods of obtaining such organized 3D cell structures from mammalian cells, and to devices, in particular microfluidic platform, to perform such methods. The invention also concerns the use of the thus obtained 3D cell structures in applications of molecule screening, developmental testing, production of physiologically active substances and models for therapeutic investigation or use.

公开(公告)号：WO2020077266A1

公开(公告)日：2020-04-16

申请号：PCT/US2019/055940

申请日：2019-10-11

Applicant: THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES ,  SINGEC, Ilyas ,  CHEN, Yu ,  SIMEONOV, Anton

Inventor： SINGEC, Ilyas ,  CHEN, Yu ,  SIMEONOV, Anton

IPC: C12N5/00 ,  C12N5/0775 ,  C12N5/0735 ,  C12N5/074 ,  C12N2501/40 ,  C12N5/0018 ,  C12N5/0606 ,  C12N5/0662 ,  C12N5/0696

Abstract: Compositions and methods useful for culturing mammalian cells, tissues and/or organs are provided. The compositions contain Chroman 1 or its derivatives, may further include Emricasan or its derivatives, and may further include one or both of trans-ISRIB and polyamines. The compositions may be formulated as media additives, media concentrates or working media. The compositions and methods may be used to culture stem cells, progenitor cells, differentiated cells, isolated primary cells, secondary cells, immortalized cells, cell line cells, germline cells, somatic cells, or modified cells, including genetically modified cells. The compositions and methods may be used to culture, preserve or maintain cells, tissues, organs parts or organs, grow embryoid bodies, embryos, organs or organ parts.