公开(公告)号：WO2021250420A1

公开(公告)日：2021-12-16

申请号：PCT/GB2021/051453

申请日：2021-06-11

Applicant: AUTOLUS LIMITED

Inventor： RUBIO GÓMEZ, Ana ,  GÓMEZ PALOU, Monica ,  CULSHAW, Abigail

IPC: C12N5/0783 ,  A61K35/17 ,  A61P35/00 ,  C12N2501/2307 ,  C12N2501/2315 ,  C12N2501/72 ,  C12N2501/727 ,  C12N2510/00 ,  C12N5/0636

Abstract: The present invention relates to a culture medium suitable for the production of T-cells comprising molecules that are able to uncouple the processes of proliferation and differentiation allowing for effective expansion of cells that maintain a naïve or 'early' memory phenotype.

公开(公告)号：WO2023002057A2

公开(公告)日：2023-01-26

申请号：PCT/EP2022/070715

申请日：2022-07-22

Applicant: INSTITUT PASTEUR ,  ECOLE POLYTECHNIQUE ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE

Inventor： BAROUD, Charles ,  COSSEC, Jack Christophe ,  DEJEAN, Anne ,  SART, Sébastien ,  TRABOULSI, Tatiana

IPC: C12N5/0735 ,  B01L3/00 ,  C12M3/06 ,  C12M1/12 ,  C12M3/00 ,  G01N21/00 ,  C12M23/16 ,  C12M25/14 ,  C12N2501/235 ,  C12N2501/40 ,  C12N2501/70 ,  C12N2501/72 ,  C12N2513/00 ,  C12N2535/00 ,  C12N5/0606

Abstract: The invention relates to in vitro generation of organized 3D cell structures recapitulating various degrees of early organogenesis, including head-trunk embryo-like structures, using epigenetic remodeling factors. The invention relates in particular to methods of obtaining such organized 3D cell structures from mammalian cells, and to devices, in particular microfluidic platform, to perform such methods. The invention also concerns the use of the thus obtained 3D cell structures in applications of molecule screening, developmental testing, production of physiologically active substances and models for therapeutic investigation or use.