公开(公告)号：WO2021254958A1

公开(公告)日：2021-12-23

申请号：PCT/EP2021/065961

申请日：2021-06-14

Applicant: BIOMILLENIA SAS

Inventor： DU, Guansheng ,  THOMAS, Laurine ,  DAJKOVIC, Aleksander ,  LOEFFERT, Dirk

IPC: B01L3/00 ,  C12M1/34 ,  C40B50/08 ,  C40B60/14 ,  C12Q1/02 ,  C12M1/12 ,  C12M1/00 ,  G01N15/14 ,  C12M3/06 ,  C40B40/02 ,  C40B60/08 ,  G01N15/00 ,  B01L2200/0652 ,  B01L2200/12 ,  B01L3/502784 ,  C12M23/16 ,  C12M25/01 ,  C12M41/46 ,  G01N15/1459 ,  G01N2015/003 ,  G01N2015/149

Abstract: In a first aspect, the present invention relates to a method of producing a library of microorganisms, the method comprising the steps of: a. providing a first fluid comprising at least one single cell, b. dispersing said first fluid comprising at least one single cell in a second fluid, thereby obtaining a plurality of single-layer microfluidic droplets, wherein at least one single- layer microfluidic droplet comprises at least one single cell, wherein the second fluid is immiscible with the first fluid, c. optionally, adding to said at least one single-layer microfluidic droplet a third fluid comprising a sensing compound, wherein the third fluid is miscible with said first fluid, and wherein the third fluid is immiscible with said second fluid, d. injecting said at least one single-layer microfluidic droplet optionally comprising the sensing compound into a fourth fluid, wherein said fourth fluid is immiscible with said second fluid, thereby obtaining at least one double-layer microfluidic droplet, e. dispensing said at least one double-layer microfluidic droplet into a culture medium based on the viability of the cell, f. incubating said culture medium, thereby obtaining said library. In a second aspect, the present invention relates to a system comprising: a. a first microfluidic chip for producing a plurality of single-layer microfluidic droplets wherein at least one single-layer microfluidic droplet comprisesat least one single cell, b. a first microfluidic device for collecting said plurality of single-layer microfluidic droplets, c. a second device for adding a sensing compound into said at least one single-layer microfluidic droplet comprising at least one single cell, d. a second microfluidic chip for producing a double-layer microfluidic droplet, and e. a dispensing unit. In a third aspect, the present invention relates to the use of the method according to the first aspect of the present invention in a system according to the second aspect of the present invention.