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    METHOD AND SYSTEM OF PRODUCING A LIBRARY OF MICROORGANISMS
    1.
    发明申请
    METHOD AND SYSTEM OF PRODUCING A LIBRARY OF MICROORGANISMS 审中-公开

    公开(公告)号：WO2021254958A1

    公开(公告)日：2021-12-23

    申请号：PCT/EP2021/065961

    申请日：2021-06-14

    Applicant: BIOMILLENIA SAS

    Inventor： DU, Guansheng ,  THOMAS, Laurine ,  DAJKOVIC, Aleksander ,  LOEFFERT, Dirk

    IPC: B01L3/00 ,  C12M1/34 ,  C40B50/08 ,  C40B60/14 ,  C12Q1/02 ,  C12M1/12 ,  C12M1/00 ,  G01N15/14 ,  C12M3/06 ,  C40B40/02 ,  C40B60/08 ,  G01N15/00 ,  B01L2200/0652 ,  B01L2200/12 ,  B01L3/502784 ,  C12M23/16 ,  C12M25/01 ,  C12M41/46 ,  G01N15/1459 ,  G01N2015/003 ,  G01N2015/149

    Abstract: In a first aspect, the present invention relates to a method of producing a library of microorganisms, the method comprising the steps of: a. providing a first fluid comprising at least one single cell, b. dispersing said first fluid comprising at least one single cell in a second fluid, thereby obtaining a plurality of single-layer microfluidic droplets, wherein at least one single- layer microfluidic droplet comprises at least one single cell, wherein the second fluid is immiscible with the first fluid, c. optionally, adding to said at least one single-layer microfluidic droplet a third fluid comprising a sensing compound, wherein the third fluid is miscible with said first fluid, and wherein the third fluid is immiscible with said second fluid, d. injecting said at least one single-layer microfluidic droplet optionally comprising the sensing compound into a fourth fluid, wherein said fourth fluid is immiscible with said second fluid, thereby obtaining at least one double-layer microfluidic droplet, e. dispensing said at least one double-layer microfluidic droplet into a culture medium based on the viability of the cell, f. incubating said culture medium, thereby obtaining said library. In a second aspect, the present invention relates to a system comprising: a. a first microfluidic chip for producing a plurality of single-layer microfluidic droplets wherein at least one single-layer microfluidic droplet comprisesat least one single cell, b. a first microfluidic device for collecting said plurality of single-layer microfluidic droplets, c. a second device for adding a sensing compound into said at least one single-layer microfluidic droplet comprising at least one single cell, d. a second microfluidic chip for producing a double-layer microfluidic droplet, and e. a dispensing unit. In a third aspect, the present invention relates to the use of the method according to the first aspect of the present invention in a system according to the second aspect of the present invention.

    SELECTIVELY CONTROLLABLE CLEAVABLE LINKERS
    2.
    发明申请
    SELECTIVELY CONTROLLABLE CLEAVABLE LINKERS 审中-公开

    公开(公告)号：WO2020131588A1

    公开(公告)日：2020-06-25

    申请号：PCT/US2019/066106

    申请日：2019-12-13

    Applicant: MICROSOFT TECHNOLOGY LICENSING, LLC

    Inventor： STRAUSS, Karin ,  NGUYEN, Bichlien Hoang

    IPC: B01J19/00 ,  C40B80/00 ,  C40B50/18 ,  C40B60/14

    Abstract: Selectively controllable cleavable linkers include electrochemically-cleavable linkers, photolabile linkers, thermolabile linkers, chemically-labile linkers, and enzymatically-cleavable linkers. Selective cleavage of individual linkers may be controlled by changing local conditions. Local conditions may be changed by activating electrodes in proximity to the linkers, exposing the linkers to light, heating the linkers, or applying chemicals. Selective cleaving of enzymatically-cleavable linkers may be controlled by designing the sequences of different sets of the individual linkers to respond to different enzymes. Cleavable linkers may be used to attach polymers to a solid substrate. Selective cleavage of the linkers enables release of specific polymers from the solid substrate. Cleavable linkers may also be used to attach protecting groups to the ends of growing polymers. The protecting groups may be selectively removed by cleavage of the linkers to enable growth of specific polymers.

    METHODS FOR FABRICATING HIGH RESOLUTION DNA ARRAY AND ITS APPLICATION IN SEQUENCING
    3.
    发明申请
    METHODS FOR FABRICATING HIGH RESOLUTION DNA ARRAY AND ITS APPLICATION IN SEQUENCING 审中-公开

    公开(公告)号：WO2019136322A1

    公开(公告)日：2019-07-11

    申请号：PCT/US2019/012444

    申请日：2019-01-05

    Applicant: CENTRILLION TECHNOLOGIES, INC.

    Inventor： CRNOGORAC, Filip ,  MCGALL, Glenn ,  DENTINGER, Paul ,  POLLOM, T. Scott ,  ZHOU, Wei

    IPC: C40B50/14 ,  C07H21/04 ,  C40B40/06 ,  C40B60/14

    Abstract: The present disclosure provides methods and processes for forming a pattern of oligonucleotides on a microarray. A method for forming a pattern of oligonucleotides on a microarray may include forming a photoresist layer by applying a photoresist composition onto an underlying layer of a substrate, exposing a dose of light through a patterned mask onto the substrate, and removing protective groups on a section of the plurality of functional groups within at least one exposed region of the substrate, wherein the photoresist composition comprises a photoacid generator, an acid scavenger and a photosensitizer, wherein the underlying layer comprises a plurality of functional groups protected by protective groups; thereby forming a pattern on the substrate, wherein the pattern comprises the at least one exposed region, and wherein the at least one exposed region is no more than 1 micrometer in at least one dimension.

    构建待测基因组的DNA测序文库的方法及其应用
    4.
    发明申请
    构建待测基因组的DNA测序文库的方法及其应用 审中-公开

    公开(公告)号：WO2017181880A1

    公开(公告)日：2017-10-26

    申请号：PCT/CN2017/080142

    申请日：2017-04-11

    Applicant: 清华大学

    Inventor： 颉伟 ,  张冰洁

    IPC: C12N15/10 ,  C40B50/06 ,  C40B60/14 ,  C12Q1/68

    CPC classification number: C12N15/10 ,  C12Q1/68 ,  C40B50/06 ,  C40B60/14

    Abstract: 提供了一种构建待测基因组的DNA测序文库的方法，该方法包括：（1）利用微球菌核酸酶对待测基因组进行第一消化处理，以便获得第一消化处理产物；（2）将所述第一消化处理产物进行免疫共沉淀处理，以便获得免疫共沉淀处理产物；（3）利用蛋白酶K对所述免疫共沉淀处理产物进行第二消化处理，以便获得第二消化处理产物；（4）利用虾碱性磷酸酶对所述第二消化处理产物直接进行去磷酸化处理，以便获得去磷酸化处理产物；（5）将所述去磷酸化处理产物直接进行变性处理，以便获得含有单链DNA分子的变性处理产物；以及（6）基于所述含有单链DNA分子的变性处理产物，按照TELP法获得测序文库。

    DROPLET DISPENSING DEVICE AND METHODS
    7.
    发明申请
    DROPLET DISPENSING DEVICE AND METHODS 审中-公开
    滴液分配装置和方法

    公开(公告)号：WO2008124846A3

    公开(公告)日：2009-12-23

    申请号：PCT/US2008059955

    申请日：2008-04-10

    Applicant: ADVANCED LIQUID LOGIC INC ,  POLLACK MICHAEL ,  PAMULA VAMSEE ,  SRINIVASAN VIJAY

    Inventor： POLLACK MICHAEL ,  PAMULA VAMSEE ,  SRINIVASAN VIJAY

    IPC: C40B60/14 ,  G01N1/10

    CPC classification number: B01L3/502761 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/043 ,  G01N27/447

    Abstract: The invention provides nonlimiting examples of structures for and methods of dispensing droplets in a droplet actuator. The droplet actuator structures and methods of the invention exhibit numerous advantages over droplet actuators of the prior art. In various embodiments, the structures and methods of the invention provide, among other things, improved efficiency, throughput, scalability, and/or droplet uniformity, as compared with existing droplet actuators. Further, in some embodiments, the droplet actuators provide configurations for improved methods of loading and/or unloading fluid and/or droplets. In yet other embodiments, the droplet actuators provide fluid loading configurations for loading numerous fluid reservoirs in a substantially simultaneous and/or substantially sequential manner.

    Abstract translation: 本发明提供了用于在液滴致动器中分配液滴的结构的方法和方法的非限制性实例。 本发明的液滴致动器结构和方法与现有技术的液滴致动器相比表现出许多优点。 在各种实施例中，与现有的液滴致动器相比，本发明的结构和方法尤其提供了改进的效率，吞吐量，可扩展性和/或液滴均匀性。 此外，在一些实施例中，液滴致动器提供用于加载和/或卸载流体和/或液滴的改进方法的构造。 在其他实施例中，液滴致动器提供用于以基本上同时和/或基本顺序的方式装载多个流体储存器的流体加载构造。

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