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    ELECTROCHEMICALLY-CLEAVABLE LINKERS
    4.
    发明申请
    ELECTROCHEMICALLY-CLEAVABLE LINKERS 审中-公开

    公开(公告)号：WO2021158412A1

    公开(公告)日：2021-08-12

    申请号：PCT/US2021/015345

    申请日：2021-01-28

    Applicant: MICROSOFT TECHNOLOGY LICENSING, LLC

    Inventor： NGUYEN, Bichlien ,  SMITH, Jake ,  STRAUSS, Karin

    IPC: G01N33/547 ,  C07C1/00 ,  C12N15/00

    Abstract: This disclosure provides electrochemically-cleavable linkers with cleavage potentials that are less than the redox potential of the solvent in which the linkers are used. In some applications, the solvent may be water or an aqueous buffer solution. The linkers may be used to link a nucleotide to a bound group. The linkers include a cleavable group which may be one of a methoxybenzyl alcohol, an ester, a propargyl thioether, or a trichloroethyl ether. The linkers may be cleaved in solvent by generating an electrode potential that is less than the redox potential of the solvent. In some implementations, an electrode array may be used to generate localized electrode potentials which selectively cleave linkers bound to the activated electrode. Uses for the linkers include attachment of blocking groups to nucleotides in enzymatic oligonucleotide synthesis.

    一种免疫电极的制备方法
    5.
    发明申请
    一种免疫电极的制备方法 审中-公开

    公开(公告)号：WO2021115491A1

    公开(公告)日：2021-06-17

    申请号：PCT/CN2020/140515

    申请日：2020-12-29

    Applicant: 南京岚煜生物科技有限公司

    Inventor： 许行尚 ,  陈杰弗瑞

    IPC: G01N33/547 ,  G01N27/00

    Abstract: 一种免疫电极的制备方法，其中免疫电极包括基层(10)、金层(11)、导电聚合物层(12)和抗体层(13)，基层(10)、金层(11)、导电聚合物层(12)和抗体层(13)从下至上依次贴合。免疫电极的制备方法，具体包括以下步骤：(1)制备导电聚合物层(12)：在镀金基层上制备聚吡咯层，获得聚吡咯/镀金基层；(2)制备免疫电极：在聚吡咯层上制备抗体层(13)，获得抗体/聚吡咯/镀金基层；(3)形成免疫电极体系：将裸镀金基层与抗体/聚吡咯/镀金基层的外侧进行固定，得到免疫电极体系。将聚吡咯材料用于固定生物识别元件抗体，将其固载在免疫电极上，由于聚吡咯具有卓越的导电能力，能够加速电极表面电子转移，提高免疫电极的灵敏度。

    BIASING SEX SELECTION
    8.
    发明申请
    BIASING SEX SELECTION 审中-公开
    偏心性选择

    公开(公告)号：WO2018053571A1

    公开(公告)日：2018-03-29

    申请号：PCT/AU2017/000201

    申请日：2017-09-20

    Applicant: BIASSEX PTY LTD

    Inventor： PFISTERSHAMMER, Josef

    IPC: A61D19/00 ,  G01N33/547

    Abstract: A method for biasing sex selection, the method comprising the introduction into a uterus of a subject a volume of micro-particle conjugates (10), wherein the micro- particle conjugates are proportioned so as to approximate the size and shape of spermatozoa and thereby be carried by peristaltic waves through the uterus and fallopian tubes to the infundibulum, at which point any spermatozoa present or arriving thereafter undergo capacitation and expose antigens that may be bound by anti-male antibodies (14) provided in the micro-particle conjugates (10), the binding of the exposed antigens on the spermatozoa by the anti-male antibodies resulting in their inability to penetrate the Zona Pellucida of an egg and effect fertilisation.

    Abstract translation: 一种用于偏置性选择的方法，所述方法包括向受试者的子宫内引入一定体积的微粒缀合物（10），其中所述微粒缀合物被按比例配置以接近 大小和形状，并由此通过蠕动波通过子宫和输卵管进入漏斗部，此时存在或到达的任何精子经历获能并暴露可被抗雄性抗体结合的抗原（14） 微粒偶联物（10），暴露的抗原在精子上被抗雄性抗体结合，导致它们不能穿透卵的透明带并影响受精。

    鉴别从人或动物生物体液中富集的非血源性有核细胞的方法
    9.
    发明申请
    鉴别从人或动物生物体液中富集的非血源性有核细胞的方法 审中-公开

    公开(公告)号：WO2013063981A1

    公开(公告)日：2013-05-10

    申请号：PCT/CN2012/081147

    申请日：2012-09-07

    Applicant: 李彦萍

    Inventor： 李彦萍

    IPC: G01N33/547 ,  G01N33/48 ,  C12Q1/68 ,  G01N1/40

    CPC classification number: G01N33/57492 ,  C12Q1/6886 ,  C12Q2600/156 ,  G01N33/56966

    Abstract: 一种鉴别从人或动物生物体液中富集的非血源性有核细胞的方法，其中同步实施抗人白细胞抗体免疫荧光染色以及染色体荧光原位杂交以区别血源性有核细胞，有效去除在判断肿瘤细胞过程中血源性细胞产生的干扰，从而达到高效快速地从人体液中富集提取并有效鉴别非血源性上皮来源或非上皮来源的稀有肿瘤细胞。本发明提供的方法不需要裂解融破红细胞，不会对人体液中的靶细胞产生影响，从而很好地保持了原有的细胞形态，便于后续的细胞识别与判断。

    MAGNETIC PARTICLE TAGGED REAGENTS AND TECHNIQUES
    10.
    发明申请
    MAGNETIC PARTICLE TAGGED REAGENTS AND TECHNIQUES 审中-公开
    磁性粒子标签试剂和技术

    公开(公告)号：WO2008033164A8

    公开(公告)日：2009-04-16

    申请号：PCT/US2007005993

    申请日：2007-03-08

    Applicant: CHROME RED TECHNOLOGIES LLC ,  GRAHAM HENRY A ,  GORMAN JOHN G ,  ROWELL JAMES P

    Inventor： GRAHAM HENRY A ,  GORMAN JOHN G ,  ROWELL JAMES P

    IPC: G01N33/553 ,  C12Q1/00 ,  G01N33/533 ,  G01N33/547

    CPC classification number: G01N33/80 ,  G01N2446/00

    Abstract: Methods for separating, in a continuous, multizone fluid medium, cells, particles, or other molecules of interest (MOI) from associated or contaminating unwanted materials not of interest (MNOI). The invention involves forced movement of MOI into certain zones having properties which deter the entry of unwanted materials. Differential movement of MOI and MNOI occurs by active counterforces that move MNOI but not MOI. MOI are tagged with magnetic particles and moved with a magnetic field through a fluid, or zones, of higher specific gravity that prevents, by flotation counterforce, unwanted less dense materials from entering. Surfaces specifically coated with reactants are reactive with the MOI in the tagged magnetic particle complex and of buoyant or other forces are used to remove any unbound material from the surface before reading. Readable labels, in addition to the magnetic particle tagged complex itself, such as enzymes, fluorophors, chemiluminescent materials, radioactive isotopes, chromogenic and fluorogenc substrates and other labels may be used. In most embodiments, materials of interest are delivered to a special final zone for reading or harvesting. The invention applies to many assays, diagnostic tests, separative procedures and chemical syntheses.

    Abstract translation: 在连续的多区流体培养基中分离来自相关或污染的不需要的物质（MNOI）的细胞，颗粒或其他感兴趣的分子（MOI）的方法。 本发明涉及将MOI强制移动到具有阻止不想要的材料进入的特性的某些区域中。 MOI和MNOI的差异运动是通过移动MNOI而不是MOI的主动抵制来实现的。 MOI用磁性颗粒标记，并通过具有较高比重的流体或区域的磁场移动，通过浮选反作用力阻止不需要的较不致密的材料进入。 特别涂覆有反应物的表面与标记的磁性颗粒复合体中的MOI反应，并且在读取之前使用浮力或其它力从表面去除任何未结合的材料。 可以使用除了磁性颗粒标记的复合物本身之外的可读标签，例如酶，荧光体，化学发光材料，放射性同位素，显色和氟代基底和其它标记。 在大多数实施例中，感兴趣的材料被输送到用于阅读或收获的特殊最后区域。 本发明适用于许多测定，诊断测试，分离程序和化学合成。

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